Protocol - pLKO.1 – TRC Cloning Vector
Type
Protocol
...B7001S NEB buffer 2 NEB #B7002S T4 DNA ligase NEB #M0202S T4 DNA ligase buffer NEB #B0202S DH5 alpha competent...concentration, use 1 μL) 2 μL 10x NEB T4 DNA ligase buffer 1 μL NEB T4 DNA ligase add ddH 2 O to bring to 20 μL final...should be from 1-10 μg/mL in 1 μg/mL increments. d. Label plates from 1-10 and add appropriate puromycin-containing...