...Lentiviral particles can efficiently infect a broad range of cell types, including both dividing and non-dividing...the Whitehead Institute for Biomedical Research hosts an siRNA Selection Program that can be accessed ...created. Additional design rules can be found at the Broad Institute's TRC shRNA Design Process . To minimize...but the DNA is unlikely to be integrated into the host genome. For stable loss-of-function experiments,...particles per cell. Addgene recommends that you test a range of MOIs to determine the optimal MOI for infection.../mammalianrnai The RNAi Consortium (TRC): www.broad.mit.edu/genome_bio/trc/rnai.html Background on RNAi...