Protocol - pLKO.1 – TRC Cloning Vector
Type
Protocol
...Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing viral particles...Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells GenHunter: #Q401...shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-free OPTI-MEM ...