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  1. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing viral particles...Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells GenHunter: #Q401...shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-free OPTI-MEM ...
  2. Lentivirus Production

    Type
    Protocol
    ...: Reagent Amount per 10 cm dish* psPAX2 1.3 pmol pMD2.G 0.72 pmol Transfer Plasmid* 1.64 pmol OptiPro ...
Showing: 1 - 2 of 2 results