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  1. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has ...searchable list here). Tips for using pMD2.G At Addgene, we use pMD2.G for producing our ready-to-use lentivirus...effects. To date, nearly 900 articles cite the use of pMD2.G obtained from Addgene. Skimming the list, one ...one gets a sense of the broad usefulness of pMD2.G and VSV-G. This plasmid has been used in studies of the... pmd2.g...
  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G envelope...’s meant to be used with pMDLg/pRRE and the envelope expressing plasmid pMD2.G, both deposited from the...pRSV-Rev and the envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from ...plasmid was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (PX458) - This plasmid from...the same lab. Find pRSV-Rev. pMDLg/pRRE - This is a 3rd generation lentiviral packaging plasmid from the...
  3. Viral Production at Addgene

    Type
    Blog Post
    ...in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab. Cell...
  4. Lentivirus Plasmids

    Type
    Collection
    ...deficient lentivirus Rawlings , Scharenberg 12251 pMDLg/pRRE 3rd Packaging Contains Gag and Pol; can be ...and 3rd generation transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid...
  5. Retrovirus Plasmids

    Type
    Collection
    ...protein; use with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono...
  6. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...regions of interest 48807 8xLexA-binding elements/pMD20 iChIP plasmid, LexA-binding elements 48874 3xFNLDD...
  7. Viral Production

    Type
    Collection
    ...in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab . ...
  8. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing viral particles...Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells GenHunter: #Q401...shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-free OPTI-MEM ...
  9. Lentivirus Production

    Type
    Protocol
    ...: Reagent Amount per 10 cm dish* psPAX2 1.3 pmol pMD2.G 0.72 pmol Transfer Plasmid* 1.64 pmol OptiPro ...
  10. Lentiviral Guide

    Type
    Guide
    ...packaging plasmid and rev from another, such as pMDLg/pRRE and pRSV-Rev . 3rd generation packaging systems...
Showing: 1 - 12 of 12 results