Protocol - pLKO.1 – TRC Cloning Vector
Type
Protocol
... tissue culture plate. Incubate cells at 37°C, 5% CO 2 overnight. Although cells should regularly be passaged...cells from the plate. i. Incubate cells at 37°C, 5% CO 2 for 12-15 hours. Day 3: j. In the morning, change... transfected cells. k. Incubate cells at 37°C, 5% CO 2 for 24 hours. Day 4: l. Harvest media from cells...antibiotics to the cells and incubate at 37°C, 5% CO 2 for 24 hours. Day 5: n. Harvest media from cells...target cells in ten 6 cm plates and grow at 37°C, 5% CO 2 overnight. Day 2: b. The target cells should be...1: a. Plate target cells and incubate at 37°C, 5% CO 2 overnight. Day 2: b. Target cells should be approximately...your target cell line. d. Incubate cells at 37°C, 5% CO 2 overnight. Day 3: e. Change to fresh media 24 hours...