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  1. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ... Digest Introduction Sequence verification of important plasmid features (such as the gene/insert, fusion...you sequence any portion of the plasmid that is important for your experiments. There are a number of factors...

  2. Weighing Reagents Protocol

    Type
    Protocol
    ... or paper. You need to do this because you don’t want to include the weight of the weighing boat or paper...Conclusion Appropriately weighing reagents is important to ensure the accurate composition of your solutions...

  3. Video Library

    Type
    Protocol
    ...Over-Agar Antibiotic Plating Create plates to culture bacteria in the lab Over-Agar Antibiotic Plating ...

  4. Fluorescence Titering Assay

    Type
    Protocol
    ... It is not recommended to subject lentiviral supernatants to multiple freeze-thaw cycles. Procedure Seed...titer lentiviral vectors (10 5 TU/mL). If you anticipate that your viral stock will be higher titer, consider...

  5. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...located before you start. Use the eyewash station if unwanted or biohazardous materials are splashed onto your...Protecting yourself and working smart in the lab is important! Disclaimer: The SOP presented here has been designed...

  6. General Transfection

    Type
    Protocol
    ...a T75 flask in a volume of 15 mL. Do not add antibiotics to the media. Use cells that are below passage...between batches of PEI depending on the user, quantities of chemical used, volumes, pH adjustment etc....

  7. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...became automated with the discovery of a heat resistant DNA polymerase from the thermophilic bacterium...are complementary to the 3’ ends of the sense and anti-sense strands of the template DNA. They are strands...

  8. Protocol - How to Design Primers

    Type
    Protocol
    ...toward one another. The size of the primer is very important as well. Short primers are mainly used for amplifying...

  9. Ligation Independent Cloning

    Type
    Protocol
    ...provided by your polymerase manufacturer. It is very important to remove all free nucleotides from your PCR product...
Showing: 41 - 50 of 50 results