... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate...questions. All Antibodies Ready to use antibodies? Browse Addgene’s collection of antibodies developed and... New to using antibodies? In our Antibody Guide, we define key terms, summarize antibody production and... use your antibody. Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols ...Overview of Addgene's ready-to-use monoclonal recombinant antibody collection. These affinity reagents are ... for Antibody Plasmids? Browse our collection of affinity reagent plasmids - including antibodies, nanobodies...reagents that are expressed from plasmids. These antibodies undergo application-specific validation and consistent...

... or use your antibody. Antibody Guide Antibody Protocols Recipient Instructions Antibody Blog Posts Addgene...distributes ready-to-use recombinant antibodies. These monoclonal antibodies undergo application-specific validation...each antibody, we provide important details including the protein and plasmid sequence, the antigen sequence...and secondary antibodies. To learn more about antibodies and their use, visit our Antibody Guide . Browse...new antibodies to add to the collection using our Antibody Suggestion Form . Browse All Antibodies ID ...Addgene's collection of recombinant monoclonal antibodies. All... All Antibodies All Antibodies Addgene distributes...

...target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody), the target antigen is first... or use your antibody. Antibody Guide Antibody Protocols Recipient Instructions Antibody Blog Posts Addgene...Addgene's antibody production methods and quality control processes for purified recombinant antibodies. Antibody...the specific antibody webpage for further information. Production For recombinant antibodies (rAb) encoded...Antibody Production Antibody Production Many antibodies distributed by Addgene have been produced in-house...specific antibody in order to generate high quality preparations. Following production, all antibody preps...below. Information for a specific antibody can be found on the antibody's catalog page or by contacting help...

... are called recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from ...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production... produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies can also ...amounts of a single antibody with little variation. Recombinant plasmids - Antibodies can be produced from...variable region and constant regions of the antibody to be independently manipulated. Antibodies produced this... in a buffer. Antibody Structure Figure 1: Structure of an antibody A standard antibody is made up of ...producing antibodies, can change the constant region, and therefore change the isotype of antibodies they ...

...Addgene's plant resources: Find plasmids for use in plants.... Plasmid Collections Plant Plasmids Plant Plasmids...may also like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR Cloning...designed for use in plants. These resources include tools for research using plant systems such as Arabidopsis...species-specific tools). Support open science in plant biology - deposit plant plasmids with Addgene! CRISPR Please...different functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR resources...in monocot and dicot plants - Yao-Guang Liu. Plasmids for prime editing in plants (including rice and ...

...Backbones for mouse recombinant antibodies A panel of recombinant monoclonal antibodies against zebrafish...Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large proteins made by the ...use synthetically produced recombinant antibodies, created by cloning antibody components into plasmids ...length recombinant monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are...about antibodies and their applications in our Antibody Guide ! Cloning and Expressing Antibodies The following...engineer the constant immunoglobulin domains to obtain recombinant hybridomas, which secrete antibodies as Fab...chimeric antibodies, and Fc-silent mutants. Functional diversification of hybridoma-produced antibodies by ...

... CRISPR Plants CRISPR Plasmids: Plants Browse CRISPR Plasmids By Function...Double nicking strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair...have been designed for use in plants. CRISPR... gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes will introduce... Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands...

... Plasmid Collections Synthetic Biology Plant Synthetic Biology: Plant SynBio Resources...collection of synthetic biology plasmids for use in plants. Plant Plasmids Search the table by keyword or sort...plasmids for use in plants. Plasmid...

...Plasmids Zebrafish Monoclonal Antibodies Zebrafish Monoclonal Antibodies You may also like... Gavin Wright...chains of mouse monoclonal antibodies to zebrafish proteins and the antibodies are produced by transfecting... collecting the secreted monoclonal antibody from the supernatant. Please see the following reference ... scalable method for selecting recombinant mouse monoclonal antibodies. Crosnier C et al., BMC Biol. 2010...collection of plasmids that encode monoclonal antibodies to zebrafish cell surface receptors and secreted...

...IgG2a 114557 Anti-TRIP8b (constant) [N269/73R] TRIP8b (constant) Rat Mouse IgG2a 114558 Anti-Synaptotagmin...IgG2a 177514 Anti-TRIP8b (constant) [N212/7R] TRIP8b (constant) Rat Mouse IgG2a 177515 Anti-RBM17/SPF45...IgG2a 177503 Anti-Pan-Gamma-protocadherin-Constant [N159/5R] Pan-Gamma-protocadherin-Constant Mouse IgG2a...applications. A substantial number of these have been cloned, expressed and validated in recombinant form. The...The recombinant monoclonal antibodies or R-mAbs have been engineered such that they are a distinct IgG... by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody Plasmid Collection The following plasmids...Isotype 114473 Anti-TRIP8b (exon 4) [N212/3R] TRIP8b (exon 4) Rat Mouse IgG2a 114474 Anti-Clcn4 [N222/6R...

...Innovation Antibody Collection Glypican Antibodies Integrin Antibodies Epitope Tag Antibodies Antibody Plasmids...well as antibody plasmids to create or express plasmid-based antibodies. The majority of antibodies in the...Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Figure courtesy of IPI Browse Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Browse Epitope Tag Antibodies IPI has developed recombinant versions of antibodies that recognize common.... ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse Antibody Plasmids...Find unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration...

...Averof pBUN6A11 50579 Plant BsaI yes, activate S. pyogenes Bar Chen pBUN6I11 50580 Plant BsaI yes, interfere...Bar Chen pBUN411 50581 Plant BsaI yes, cut S. pyogenes Bar Chen pBUN501 50582 Plant BsaI yes, nick S. pyogenes...Chen pHSN6A01 50586 Plant BsaI yes, activate S. pyogenes Hyg Chen pHSN6I01 50587 Plant BsaI yes, interfere...Chen pHSN501 50589 Plant BsaI yes, nick S. pyogenes Hyg Chen pCBC-DT3T4 50592 Plant BsaI none S. pyogenes... Chen pCBC-MT1T2 50593 Plant BsaI none S. pyogenes Chen pCBC-MT2T3 50594 Plant BsaI none S. pyogenes Chen... Chen pCBC-MT3T4 50595 Plant BsaI none S. pyogenes Chen pBUN421 62204 Plant BsaI yes, cut S. pyogenes ...Bar Chen pU3-gRNA 53063 Plant AarI none S. pyogenes Gao pZmU3-gRNA 53061 Plant none S. pyogenes Gao pU6...

...Ca 2+ binding domain. Nano-lanterns , Enhanced Nano-lanterns , and Antares : Fluorescent proteins are ...Renilla luciferase (Nano-lanterns) or NanoLuc® (Antares and Enhanced Nano-lanterns) to allow for BRET-based...Liqun Luo 51970 Nano-lantern/pcDNA3 Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121...systems. Each type of luciferase has advantages and disadvantages depending on the application. Firefly...Luciferase Blog: Luminescent Imaging with Nano-lanterns Collection Highlights Empty Backbones Expression...use the red-shifted substrate Akalumine with significantly brighter signal and emission in the near-infrared...Conformationally destabilized Firefly luciferase mutants : A set of three sensors that are increasingly ...

...binding) SpCas9 VRER variant 3' NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9...LbCpf1) 5' TTTV AsCpf1 RR variant 5' TYCV LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter...PAM sequence often found in plants, expanding the targeting range for plant genome engineering. Read more...more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity can increase the risk of off-target cutting...like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The CAPTURE system, developed by the Jian...Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' ...Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

...-related antibodies including: Anti-KChIP1 K+ channel [K55/7R] Anti-Fig4/Sac3 [N202/7R] Anti-Ankyrin-G.... See More Antibodies Find information on Addgene's collection of plasmid-based antibodies, including ...-G [N106/36] Generate anti-kinesin recombinant scFvs . Niwa et al. Cytoskeleton. 2023 Apr 10. Explore ...Explore the recombinant monoclonal antibodies and scFvs for neuroscience research created with high-volume hybridoma... AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative...common disorders followed by a list of some of the relevant collections available at Addgene. Amyotrophic ...familial cases, usually inherited in an autosomal dominant manner, are associated with known genetic mutations...

...specific antigen, it finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced...recognition by T cells of foreign antigens on the surface of antigen presenting cells (APC) including ...immunity through the secretion of antibodies, and also function as antigen presenting cells. B cells in secondary...immunoglobulin heavy constant alpha 2 (A2m marker) - IGHD immunoglobulin heavy constant delta FLJ00382, FLJ46727...IGHE immunoglobulin heavy constant epsilon IgE IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) -...

...learn more about antibodies and their use, visit our Antibody Guide . ID Recombinant Antibody Description ...Find recombinant antibodies targeting neuroscience-related proteins. Antibodies...Neuroscience Antibody Collection Browse or search the table below to find antibodies currently available from...neuroscience research. These ready-to-use recombinant monoclonal antibodies undergo application-specific validation...our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or e-mail [email protected]... Antibodies Neuroscience Neuroscience...Applications PI Do you have suggestions for other antibodies that should be added to this collection? Check...

...learn more about antibodies and their use, visit our Antibody Guide . ID Recombinant Antibody Description ...Find recombinant antibodies targeting epitope tags and common fusion proteins. Antibodies... Other Markers Antibody Collection Browse or search the table below to find antibodies currently available...cellular proteins. These ready-to-use recombinant monoclonal antibodies undergo application-specific validation...our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or e-mail [email protected]... Antibodies Tags and Other...Applications PI Do you have suggestions for other antibodies that should be added to this collection? Check...

...found on this page: SARS-CoV-2 Antibodies : Recombinant monoclonal antibodies that target SARS-CoV-2 nucleocapsid...research. Antibodies Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse monoclonal...) Ectodomain and RBD mutants that can be used to identify antibody escape mutants and for comprehensive...monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Anti-SARS-CoV-2 Nucleocapsid... Protein [mBG86] - Clone mBG86, recombinant mouse monoclonal antibody targeting the SARS-CoV-2 nucleocapsid...from Ray Owens' lab. Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza. Abbott...Libraries - Libraries of Spike (S) Ectodomain and RBD mutants (Timothy Whitehead). SARS-CoV-2 Spike Receptor ...

...Dynamics of Cytosolic Redox Metabolism in Plants. Plant Cell. 2020 Aug 13. pii: tpc.20.00241. Markus Schwarzländer... potential Quantitative measurements of membrane charges A FRET sensor enables quantitative measurements...that many of the articles linked below include variants with different binding affinities or negative-...turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium. Nat Commun... Calcium Yellow protein calcium sensor jYCaMP1 variants (Constitutive or Cre-dependent) jYCaMP: an optimized...genetically encoded ratiometric calcium sensor enables quantitative measurement of the local calcium microdomain... small molecule indicator: implications for quantification of cytosolic Zn(2+). ACS Chem Biol. 2013 Nov...