...correspond to sequences on the template molecule (must be complementary to template strand). However, primers...to design primers that are complementary to the template region of DNA. They are synthesized chemically...primers do not need to correspond to the template strand completely; it is essential, however, that the 3’...end of the primer corresponds completely to the template DNA strand so elongation can proceed. Usually ...

...your LB agar plates are not fresh, you should streak your bacteria onto a new LB agar plate before growing... Streaking and Isolating Bacteria on an LB Agar Plate Creating Bacterial Glycerol Stocks for Long-term...commonly used to culture bacteria in the lab. LB agar plates are frequently used to isolate individual (clonal...toothpick, select a single colony from your LB agar plate . Drop the tip or toothpick into the liquid LB +...

...new window) Link to Video Making LB Agar Plates Create plates to culture bacteria in the lab Watch the...Video! Over-Agar Antibiotic Plating Quickly add antibiotic to a pre-poured plate Watch the Video! Streaking...Bacteria Isolate single bacterial colonies on an agar plate Watch the Video! Inoculating a Liquid Bacterial ...

...with shaking platform set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents...water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved. Note: ...flask.Incubate in a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm. Pro-Tip Do not use cells that ...

...days. Single cells were then plated in individual wells of a 96-well plate and left undisturbed for 13 ... well (each well gets one dilution, so a 6-well plate will hold 5 dilutions plus one 'no virus' control...

...competent cells and retransform. Bacteria on an LB agar plate can be stored at 4°C for a few weeks. However, if...stock unthaw! Streak the bacteria onto an LB agar plate . Grow your bacteria overnight at the appropriate...

...(μL) Volume of Virus Added to Plate (μL) Volume of Cells Added to Plate (μL) Final Viral Dilution 1:10...

...controller Hazardous waste container pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex...reach 100% confluence (80–90% is ideal). Pass and plate the cells the day before the transfection. Thaw ...

..., it is a good idea to place the gel on a glass plate if available. Unlike the plastic tray, this will...

...2-3μL into your favorite competent bacteria and plate. Be sure to pick multiple colonies for mini-prepping...

...sequence is used, plus a minimum of 18 bp of your template sequence. 5' and 3' primers will have different...

...liquid into different tubes or into wells of a microplate, you may consider using a multichannel pipette...

...bacterial culture. For details on how to streak a plate to get individual colonies and to generate liquid...

...spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead bath Clamp stand and...