...most commonly used is the Illumina NGS platform. This is the platform used by Addgene’s sequencing partner...of DNA, or templates, are spatially separated from each other. These attached DNA templates are then amplified... again producing ~1,000 copies of each template. Each template is then replicated using the modified bases...antibiotic(s) to add to your LB media or LB agar plates. Antibiotic Recommended Stock Concentration Recommended...the 4 nucleotides, a DNA polymerase enzyme, the template DNA to be copied, and a primer. A primer is a ...is a high-throughput, multi parallel sequencing platform that can generate sequencing data for up to 600...normal” bases in the reaction. All the sequenced templates are then aligned to each other to assemble the...

...genomic edits by copying from a DNA donor template . The donor template contains the desired sequence and is...linear dsDNA templates work better for large edits or insertions. When designing the donor template, incorporating...detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform, termed...pathway. HDR relies on copying DNA from a matching template to accurately repair or fill in the missing sequence...the break ends without the need for a homologous template. Both NHEJ and HDR have their uses, but we’ll ...binding site (PBS) and the desired edits on a RT template. The PBS base pairs to the 3’ flap, and the desired...driving mismatch repair using the edited strand as a template PE4/5 - improved PE2/PE3 to inhibit mismatch repair...

... Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that uses standard curves to quantify...Overview of application Coat antigens to the well of a plate, either by charge interactions (direct) or by a ...several wash steps. Add primary antibody to the plate. After incubation, wash to remove unbound antibody...Activate the conjugate and read the output via a plate reader. Special considerations The two most common...of interest is attached to the well of a 96-well plate. A conjugated primary antibody is used to bind to...be sorted (separated and deposited into tubes or plate wells) by fluorescence activated cell sorting (FACS...

...produced. The antisense strand is referred to as the template strand or non-coding strand as this is the strand...promoter region stably and transcription of the template strand can initiate. The TATA box is a DNA sequence... is the RNA responsible for providing a stable template for the translation of a protein. RNA polymerase...

... the edit should be made HDR requires a repair template and displays reduced efficiency compared to NHEJ...

...High-efficiency blue-shifted channelrhodopsin from Platymonas subcordiformis 445 CoChR Channelrhodopsin from...