Lentivirus Production
Type
Protocol
...week: Monday: Plate 1×10 6 cells in a T75 flask in 15 mL DMEM Complete. Wednesday: Plate 1×10 6 cells ...cells at 3.8×10 6 cells per plate in DMEM Complete in 10 cm tissue culture plates. Incubate the cells at 37...previously seeded 10 cm plate. Slowly pipette the transfection mix onto the 10 cm plate, being careful not ...controller Hazardous waste container pH meter Stir plate Magnetic Stir Bar Reagents DMEM high glucose, Corning... in a T75 flask in 15 mL DMEM Complete. Friday: Plate 8×10 5 cells in a T75 flask in 15 mL DMEM Complete...