... The above graph displays the stock concentration of Carbenicillin stock used (150 µL per plate). Please... concentration (100 mg/mL, 1000x) carbenicillin stock solution in sterile water (or other antibiotic) ...

...because bacteria are used as the means for both storing and replicating plasmids. Because of this, nearly...for transforming plasmid DNA for the purposes of storage and amplification. Higher efficiency cells are ... (containing the appropriate antibiotic ) from storage at 4°C and let warm up to room temperature and ...

...heat inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS...it is recommended that you titer from the frozen stock to account for any loss in titer associated with... to Plate (μL) Final Viral Dilution 1:10 100 of Stock Virus 900 150 1,350 1:100 1:100 100 of 1:10 900 ...

...posted sequence in the "View Sequence" link. Is your custom primer found within the Addgene sequencing results...require additional sequencing and need to design a custom primer, Addgene recommends using Addgene's sequencing...

... reaction, in which a single dNTP is included to stop the exonuclease function of the enzyme and shift...vector (shown in blue here). The T4 reaction may be stopped by heating to 75° for 20 minutes. Step 6: Anneal...

...enzymes Diagnostic Restriction Digest Protocol Storing and Handling Addgene Plasmids What to do after ...receive your plasmids from Addgene Instructions for Storing and Handling Plasmids Genomic Deletions with CRISPR...

...Watch the Video! Creating Bacterial Glycerol Stocks Store bacterial strains or plasmids for long term ...

...nM DAPI working solution: Prepare a 300 µM DAPI stock solution by diluting 2.1 µL of the 5 mg/mL DAPI ...working solution by diluting 5 µL of the 300 µM DAPI stock solution into 5 mL PBS. Protect from light. Procedure...

... heat-inactivated FBS and 5mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and FBS lots can...and have been sufficiently expanded, prepare cell stocks and/or harvest to test for protein expression. ...

... want to clone from the start codon (ATG) to the stop codon (TGA, in this example). Assuming you are ...taking the final 18bases of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding...

... as follows: In the Chart Editor , select the Customize tab. Select the Series drop down menu. Select ...trendline in Google Sheets as follows: In the Customize tab, select the Label drop down menu and choose...

...of materials for an experiment or reagents for a stock mixture is an essential laboratory technique, as...

...unbalanced or improperly loaded centrifuge. Immediately stop the centrifuge and investigate the issue. When you...

... aliquot individual tubes of 5, 10 or 20μL for storage at -20°C. Whenever you need to set up ligations...

... up your work area and microscope after use and store the microscope appropriately....

...microcentrifuge tube. Use the lysate immediately or store at -80 °C until ready to use. Section 2: Determine...

...heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Polyclonal stable cell pool: see our protocol...

...in representation compared to the pre-amplified stock. A last option is to perform a gel extraction of...