...from the Structural Genomics Consortium (SGC) Stockholm collection...Genomics Consortium Plasmids Browse SGC Stockholm Collection Structural Genomics Consortium Plasmids Structural...Structural Genomics Consortium Plasmids - Stockholm Plasmid Gene/Insert Vector Type Mutations Tags Industry...

... CRISPR CRISPR History CRISPR History and Development for Genome Engineering ...various CRISPR-associated (Cas) genes to not only store a record of invading phages but also to destroy ...technology development has occurred at a truly astonishing pace, with work directed towards increasing on-target...

...tend to have a story structure: beginning, end; cause, effect; lesson, learning. Storytelling is a crucial...even tell short stories during weekly lab meetings. When scientists tell good stories, they engage their...appreciation for their research. Long story short, telling a good story is something that can be learned, ... your career. Story Telling at work Don’t believe me? Here’s an example of storytelling to answer an informational...conclusion. Advice for Telling a Good Story Start at the start Any good story needs a solid point to kick off...of versions of your story and find the one that fits your audience. Enjoy the story you’re telling There... form of a story, and you’ll very quickly get better at telling it. End at the end A story needs a good...

...with Tim Miles: While not technically a research story, I couldn't resist adding in this excellent Q&A ...try in your own work!) If you've got a research story you want to share on our blog, please reach out ...

...are doing is telling a (nonfiction) story. For there to be a story, you need to have conflict. There are...complete your story: an introduction, and a resolution.   Fig. 2: The structure of a story - not just...unrelated stories that they then tuck the science into. There are almost limitless storytelling options,...pursuit of achieving knowledge. Or, as we in the storytelling business like to say, science is all about navigating...inherent tension, is interesting. It’s what drives a story forward - and this drive is so powerful that it ... communications, but they’re firmly part of the story to the scientists doing the work.  And while these...illustrates the value of the work and then build a story around them. A good rule of thumb is to find the...

...Pre-CRISPR  Having visualized and understood the DNA, the next milestone for the molecular biologist was...Ridley Scott directed Blade Runner (1982). It is a dystopian film that revolves around synthetic humans called...year after the discovery of meganucleases. The storyline involves the resurrection of dinosaurs by extracting...more blog posts about CRISPR Learn more about the history of CRISPR Read about other genome editing methods...200304010-00015 Wood AJ, Lo T-W, Zeitler B, Pickle CS, Ralston EJ, Lee AH, Amora R, Miller JC, Leung E, Meng X...

....  Storage   Once you get your exciting new antibody, the second thing you’ll want to do is store it ...what temperature to store your antibodies at, but it also matters what they’re stored in - their buffer....Resources References Boston University Flow Core. Fluorochromes Brightness Chart. Boston University Flow Core...as well. In this post, we’ll touch on antibody storage, buffer considerations, and give just the lightest...the way, you can put your antibody in short term storage (think days to weeks) in the 4°C. If you’ll only...primarily used in Western blots and ELISAs, cannot be stored in sodium azide at all, as it inhibits HRP. In ...sensitive, so antibodies conjugated to them need to be stored either in a dark, opaque vial or wrapped in aluminum...

...Addgene is announcing we have chosen to stop all activity on X, formerly Twitter. If you are interested...posting on November 20, 2023, and as of today, will stop posting, monitoring, and resharing content on X....

...futureofresearch.org/boston/. The Future of Research Symposium A year ago, trainees from around Boston met to discuss...BEST programs, including local schools in Boston: the Boston University School of Medicine and the University...The second Boston Symposium on the Future of Research will be held from 22-24 October. This blog has ... to be held at the Future of Research Symposium Boston 2015 – Academic Data and the Labor Market. For ...week, postdocs and students will again gather in Boston - this time with the aim of finding solutions to...great panels at the Future of Research Symposium Boston 2015 where we hope to begin developing concrete...participate in the Future or Research Symposium Boston 2015 you can register here or live stream the event...

...is a new software tool used to design gRNAs for custom CRISPR screens targeting a (long or short) list...Glance!  CRISPR knockout screens: Full genome vs. custom screens Full genome CRISPR knockout screens have...hypothesis driven research question. Examples of custom CRISPR screens Differentially expressed genes  ...massive amount of descriptive transcriptomics data. Custom CRISPR screens designed to target differentially...microbe or a cytokine is added to a cell). With a custom CRISPR screen and the appropriate phenotypic readout...surface proteins. One of the first challenges with a custom screen approach is to identify an appropriate list...the Output_Short file directly. We have used CustomArray, Inc for this purpose.  The gRNA oligos are...

...science stories. Here are some of our favorite spooky science stories. Six spooky science stories Zombie...Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways ...

...a Lox-Stop cassette, resulting in the neurological deficits observed in Rett Syndrome. Restoring the MECP2...to Rett Syndrome, this blog post describes the history of Rett Syndrome research, the role of the MECP2... MECP2 gene by excision of the Stop cassette using tamoxifen-inducible Cre not only halted the neurological...precise amount of protein to each brain cell to restore physiological levels. Much research is now focused...

...efforts is the Golden Stopwatch Award to celebrate exemplary TTOs. Our new Golden Stopwatch Award recognizes...certain rights and protections for depositor and requestor alike. That’s why Addgene works closely with TTOs...agreement (usually) must be executed. The Golden Stopwatch Award highlights TTOs that accelerate science ...agreements quickly! If your TTO receives a Golden Stopwatch, consider sending an email to thank them--after...examples if possible Tell them about the Golden Stopwatch! Any organization that deposits with and/or [email protected] Congratulations to the Golden Stopwatch awardees! Congratulations to all of the winners...College of Medicine  Baylor College of Medicine Boston Children's Hospital  Brandeis University  Broad...

...use in the laboratory. The discovery of GFP The story of how fluorescent proteins like GFP came to be ...

...attended and sponsored the Future of Research (FOR) Boston Symposium 2015. I personally participated as a ... Research Symposium On the FOR website, the FOR Boston 2015 organizers indicate that the main question...information gathered by Postdoctoral Associations around Boston - From Panel Session 2: Defining the Postdoc: An... Career Path Panel Two of the panels at the FOR Boston 2015 symposium, the Career Path Panel and the Early...type of failure. Final thoughts The FOR Symposium Boston 2015 shined a light on some of the many problems... Resources at Addgene  Read David Riglar's FOR Boston 2015 Blog Post Read Our Career Blog Posts Read ...

...system that utilized various cas genes not only to store a record of invading phages but to also destroy ...

...These are stormy times. It feels like we’re being buffeted by wind and waves and sometimes wonder if ...would be fully open and available to industry requestors as well. This is a time when we need as much ...Initiative was able to step in to be our “port in the storm.”  "Addgene is a unique resource for scientists ...whatever lies ahead this year. We can see signs of the storm passing. We’ve recently piloted Addgene’s new cloning...

...Addgene’s customer service philosophy Customer service can be a hassle. You finally make it through a...of our core values here is to provide unmatched customer support, as this is vital to ensuring that our...technical, or quality control issues. Improving customer support with self service We strive to make our...your busy schedule, you may not have the chance to stop what you’re doing and make a phone call or write...waiting for an answer. Luckily, there’s a trend in customer support to address this exact problem, and it’... customer support...

...Learn more about the history of plasmids and cloning: CSHL Meeting: Plasmids: History & Biology The Joshua...different experiments. Addgene was founded in order to store, QC, curate, and distribute them all in the name...eBook! In keeping with our goal of creating a one-stop reference guide for plasmids we've updated and added... history of plasmids...

...'s been about 14 years since I last attended a Keystone Meeting – far too long. Holding these meetings...course of the day. His talk, which focused on the history of genome engineering from ZFNs through TALENs ...synthetic biology. I've been answering biology customer service questions for nearly 7 years, and my colleagues...represented engineering first and foremost, from the custom software used to design complex genetic circuitry... of the book on my way to the meeting, and its history of code making and code breaking is wonderfully...increasingly important field.  Interested in Addgene's Keystone Poster?  We'll be sharing the poster with our...