... CRISPR Generate genomic deletions in mammalian cells lines using CRISPR/Cas9 Genomic Deletions with CRISPR... virus from a preparation produced in mammalian cells AAV Purification by Iodixanol Gradient Ultracentrifugation... the lab. Blog post: 10 Basic tips for mammalian cell culture Aseptic Technique Aseptic technique is a...fields such as microbiology, molecular biology or cell biology. Learn some of the key strategies of aseptic...

...how to affinity purify recombinant antibodies from cell culture supernatant using Protein A or Protein G...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...after use. Carefully pour the filtered and diluted cell culture supernatant into the Gravitrap column. Collect...

...bacteria, preventing damage to the cell membranes and keeping the cells alive. A glycerol stock of bacteria... and you will not need to obtain more competent cells and retransform. Bacteria on an LB agar plate can...

...like microbes in a drop of pond water or the cells in your cell culture dish. Microscopes come in a huge ...transmission electron microscopes that allow us to see cellular components with science-fiction levels of detail...

...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...samples may not have been processed correctly in the cell (e.g., failure to cleave the signal peptide) and...Scatter chart. Change the data range and select the cells where the x-axis is represented. Add a linear trendline...

...complete plasmid can be transformed into bacterial cells for propagation. The majority of ligation reactions...Interpretation Uncut vector - Checks viability of competent cells and verifies the antibiotic resistance of the plasmid...

...suitable for virus purification and isolation of cells, organelles, lipoproteins, and macromolecules. Importantly..., iodixanol is inert and non-toxic to mammalian cells. Therefore, and unlike other density gradient media...

...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...shaker ELISA plate washer (optional) Scale Microsoft Excel or similar software Reagents Purified antigen Purified...

...restriction digests needed in molecular biology, and thaw cell lines. There are many different types of water baths...

...min at 12,000 g. Notes: Pellet contains proteins, cell fragments, salt and other extra particles from solutions...

...Note: (Link opens in a new window) Primer3 is an excellent resource for choosing primers. Place thin-walled...