...in which the cell line was described. Using Cellosaurus Cellosaurus is searchable by cell line name (recommended...resource called Cellosaurus, a growing cell line information database describing nearly 145,000 cell lines. Cellosaurus... Cellosaurus Cellosaurus seeks to identify and describe all cell lines used in research, using four methods... related cell lines when annotating a cell line Community submissions via email: cellosaurus@sib.swiss...with cell line catalogs and collections. Importantly, Cellosaurus also identifies problematic cell lines...HeLa cells. Image courtesy of Josef Reischig.  Contributing to Cellosaurus Generated a new cell? Or ... about a cell line you’ve used? Submit your manuscript or data to Cellosaurus via cellosaurus@sib.swiss.This...

...Tracking lineage and cell identity at the single-cell level with CellTag CellTagging is a lentiviral based...individual cells. Each cell is labeled with 3-4 tags to increase the diversity of the CellTag library. ....    Find CellTag libraries here!  Applications of CellTag The Morris Lab developed CellTag to track the...reprogrammed cells based on single-cell gene expression patterns. The lab has also used CellTags for tagging...et al., 2019). Key takeaways of CellTag CellTag is a combinatory cell-indexing tool that combines scRNA-seq...describes the process where differentiated cells are turned into a cell type of choice while bypassing the intermediate...majority of cells failing to develop the desired identity. The development of single cell technologies...

... horizontal cells, and Cre-dependent-GFP expresses in horizontal cells and ganglion cells, only the horizontal... in retinal cells. To do this, the group developed a new strategy for finding retinal cell-type specific...and high cell-type specificity.  Strategy #3 (ProC): Create promoters by repeating mouse cell type specific... to test for cell type-specific expression of GFP. Half (113) expressed in retinal cells four weeks post-injection...targeted expression in specific cell types (ex: rods or cones) or cell classes (ex: photoreceptors which...occurred in one type of cell More than 50% of expression occurred in one type of cell, with the remaining ...ability to target a cell class in mice is not a good predictor for targeting the same cell class in NHPs (...

...quality work in cell culture. The problems with cell lines Simply put, the majority of cell line problems...HeLa cells, the first immortalized human cell line. He proved this by examining whether the cell lines...Cancer Cells, Marker-independent identification of glioma-initiating cells. The International Cell Line ...working cell bank has been made by a research institution (an excellent practice), or maybe cells have been...Animal Cell Culture Guide Thermofisher Guidelines for Maintaining Cultured Cells Invitrogen Cell Culture... Outreach Scientist Nick Amiss. Cell culture in the present day Cell culture is a widespread tool used... ability to propagate viruses in cell culture; if there were no cells to infect, there would be no viable...

...should start celebrating papers and grants too with pizza or pub.” The Cepko Lab celebrates any fellowships.... Once goals are reached, how do science labs celebrate accomplishments and their team's hard work? Do...team and manage work-life balance. How do you celebrate your lab’s achievements? Tom Ellis highlights ...to the pub. Dr. Ellis adds “we probably don’t celebrate enough... I’ll ask my team at our next group meeting...dinner and champagne in the lab. The Church Lab celebrates successful moments in the lab with parties in...professional growth along the way. Take time to celebrate the lab’s achievements and enjoy time with friends...to have fun outside the lab! How does your lab celebrate each other's accomplishments? Any tips on making...

...Addgene has so much to celebrate! As we enter this new growth phase of the organization and the expansion...teams continue to keep up with the demand with excellence and dedication. We don’t have worry or wonder...right? Upgrades to the internal LIMS pages and excellent work on our external website appearance and functionality...support. The Addgene management team leads with excellence. They are one of the main reasons I have so few...Addgenies. I never worry about their dedication, excellent intentions, or incredible drive to succeed.  Thanks...

...types of primary mouse cells, including neuronal and gut stem cells, dendritic cells, embryonic fibroblasts...fibroblasts, glia cells and neurons, and cell death post-transduction is low. Neuronal stem cells transduced... Primary cells recapitulate the natural biology of a cell type of interest better than immortalized lines...introduce a gene of interest into primary cells, so most primary cell lines require viral infection. A new ... primary cells. What if, instead of supplying a DNA construct, they could simply induce cells to take ...primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem cells, they...RJ, Rehmann H, Geijsen N. Cell. 2015 Apr 23;161(3):674-690. doi:10.1016/j.cell.2015.03.028. PubMed. Find...

...times), depending on the cell line so old cells should be retired! Conversely, cells younger than the 3rd ...Make sure cells don't stay out too long While you’re preparing things, make sure your cells don’t stay...contaminants. Set up a routine to split cells Don’t be lazy about splitting cells, instead try to form a routine...’s best to split cells before the plates are more than 80-85% confluent because cells like their space...lactic acid (a by-product of cellular metabolism that is toxic to the cells), and need to be split very...numbers! When starting off with a new cell line, grow the cells, aliquot them into a number of vials, ... Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with the Agarose Method Plasmids...

... a wide variety of cell lines, from HEK to CHO cells to neural and endocrine cells such as AtT-20, PC12...then handled. Additionally, many cell lines, especially the endocrine cell lines we most commonly work with...specific cell line, and its transfection efficiency. We often trypsinize the transfected cells on the day...increased). For some cells, add a drop of serum to aid in outgrowth. Depending on the cell type, we sometimes...growing cells such as HEK and CHO do not seem to require this, while nearly all endocrine cells do. While...difficulty deriving single clones from SH-SY5Y cells, as these cells did not seem to form discrete colonies but... of Medicine. In the Lindberg Lab we often make cell lines that overexpress genes of interest; more recently...

...of Multiple Kinase Activities in Live Single Cells." Cell 157, no. 7 (2014): 1724-734. PubMed PMID: 24949979...Signal transduction pathways are a lot like cell phone networks. Protein kinases deliver messages to ... dimeric Dronpa is less prone to aggregation in cells compared to the tetrameric Dronpa it was derived...inhibitors, psMEK1 and ERK KTR-mRuby2 expressing cells were incubated with an inhibitor of choice, exposed...inactivation, and then decreases. Treatment of the cells with a PP1/PP2A inhibitor leads to maintenance of...Raf-MEK-ERK signaling in the hypodermis and gut cells of C. elegans causes the same phenotype. Activation...signaling pathway with psRaf1 or psMEK1 in these same cells by 24-48 hours exposure to blue light also induced...

...researchers facilitate and accelerate scientific discovery. How can Addgene help accelerate your research? Let...Addgene’s executive director, said during our celebratory lunch, it’s hard to picture one million plasmids...assuming 400 boxes fit in a freezer). Our Addgene celebration was so much fun! Here's the Addgene Leadership...

...photocleavable gRNAs. Molecular Cell 81, 7, 1553-1565 (2021). https://doi.org/10.1016/j.molcel.2021.02.007 Moroz-Omori...evolved ways to prevent degradation of important cellular RNA molecules with post translational modifications...slightly more efficient and less toxic to mammalian cells (Allen, et al., Frontiers in Genome Editing).  Some...transfection efficiency and even track localization in cellular experiments. There are many suitable dyes to choose...enhance CRISPR-Cas genome editing in human primary cells. Nat Biotechnol 33, 985–989 (2015). https://doi....Enhance CRISPR Genome Editing Systems in Mammalian Cells. Frontiers in Genome Editing, 28 (2021). https:/...

...Institute for Cell Science. Learn more by visiting the Allen Cell Explorer at allencell.org and the Allen... hiPS cell lines generated using these plasmids, please visit the Cell Catalog on the Allen Cell Explorer... tagging to elucidate cellular structures. Overexpression methods flood the cell with protein, which can... in human cell lines. Furthermore, the quality control necessary to ensure the edited cells are behaving..., and the stem cell lines we’ve made using them are available as part of the Allen Cell Collection at ... and images generated from these cell lines can be found in the Cell Catalog on our data portal. The plasmids...

...prevent stem cell lines from becoming the new HeLa cells?), genome engineering and stem cells, and the difficulty...opportunity to see Shinya Yamanaka celebrate the tenth anniversary of his landmark Cell paper showing that it was...generate induced pluripotent stem cells (iPSCs) capable of forming nearly all cell types in the body from otherwise... Podcast Interview with Stem Cell Researcher Darrell Kotton 2. Stem cell scientists care about patients... happening. Stem cells scientists have certainly not lost site of that. 3. Stem cell science is hard, ...otherwise terminally differentiated cells simply by expressing four proteins Oct4, Sox2, Klf4, and cMyc...sharing of his OSKM factors have affected the stem cell community – a community largely created due to Yamanaka...

...that already do mammalian cell culture. HEK-293T cells are the most common cells used in viral production...versus insect cells for viral production Consideration Mammalian cells Insect cells Viruses AAV ...type of cells that will be used to make the virus. Viruses can be produced in many different cell types,...produced in bacteria cells. However, some viruses can be produced in multiple different cell types, including...you make an informed decision. Mammalian cells Mammalian cells are a great option for viral production,...production in mammalian cells is relatively quick. After transfection, HEK-293T cells start producing virus...using qPCR or digital droplet PCR. Insect cells Insect cells are a less common option for the generation...

...varying across cell types. Variance can be caused by cell cycle rates; rapidly diving cells will have higher... of your cells. Enrich S-phase cells HR operates predominantly in S and G2 phase of the cell cycle, when...HDR events, ensure cells are actively cycling through the cell cycle stages. If cells are overly confluent...way to make a knock-in cell line is to utilize the built-in repair pathway cells already have for repairing...than quiescent cells. It is important to know that HR is often mutated in cancer cells, specifically in...keeping an eye on those cells! To take this one step further, you can inhibit cell cycle regulatory proteins...negative outcomes on viability if cells are arrested too long. Prolonged cell cycle arrest can lead to apoptosis...

... do so, their T-cells were grown in vitro, their mutations corrected, and the T-cells were transferred...functioning T cell repertoire (1). In this example, it was possible to transduce the T-cells outside the...a wide range of cell types including but not limited to muscle, liver, and brain cells. There are a multitude...chain (c) and is characterized by a lack of immune cell development and function. To date, gene therapy ...that can deliver gene therapies directly to the cells that need them. Adeno-associated virus (AAV) – based...delivery of nucleic acid cargo (DNA or RNA) to target cells has been challenging. A variety of vector delivery...expression over many years in postmitotic, long-lived cell types. AAVs in the clinic Thus far, AAV vectors...