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  1. Fluorescence Titering Assay

    Type
    Protocol
    ...that can be used to titer lentiviral preparations carrying a fluorescence marker....can be used to titer lentiviral preparations that carry a fluorescence marker. Determining the titer of ...

  2. Protocol - Bacterial Transformation

    Type
    Protocol
    ...even those designed for mammalian cell expression) carry both a bacterial origin of replication and an antibiotic...overnight. Tips and FAQ How can I save time when carrying out transformations? If you are not concerned ...

  3. Pipetting Protocol

    Type
    Protocol
    ...touch the sides of the container with the pipette. Discard the pipette tip by holding the pipette tip over...If pipetting biohazardous materials, be sure to discard into a biohazardous waste container. Conclusion...

  4. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... process is shown graphically in the following cartoon, in which we are adding EcoRI and NotI sites to...Verify your Plasmid by Sequencing PCR based cloning carries a much higher risk for mutation than restriction...

  5. Western Blot

    Type
    Protocol
    ...microcentrifuge tube. Centrifuge for 5 min at 100 x g . Carefully remove supernatant. Resuspend cell pellet in appropriate...transfer stack. Set the Top Stack to one side and discard the white separator. Keep the Bottom Stack in the...

  6. Ligation Independent Cloning

    Type
    Protocol
    ...restriction sites from the final product (no "cloning scars"). A “stuffer” sequence allows for electrophoretic...

  7. Centrifugation

    Type
    Protocol
    ... When you are finished spinning your samples, carefully remove them from the centrifuge so as to not dislodge...

  9. Gibson Assembly Protocol

    Type
    Protocol
    ...almost any two fragments regardless of sequence. No scar between joined fragments. Fewer steps. One tube ...

  10. Colony Formation Titering Assay

    Type
    Protocol
    ...the stain. Wash cells 3x with 1 mL of PBS, being careful not to disturb the colonies. Count the colonies...
Showing: 21 - 32 of 32 results