... can scrape plates more abrasively at a certain angle. Incubate plates upside down at 30 ℃ overnight. ...DNA will vary upon intended use. Typically yields range from several hundred micrograms to a milligram of...taken to perform adequate NGS based QC to ensure no change in representation compared to the pre-amplified...

...material that you are weighing by looking for a weight range on the scale. Make sure that the weight of the material... material you’re weighing out is within this range. If you need less than a gram of material, use an analytical...

...that will help to maintain the plasmid without rearrangement of the plasmid DNA. Additionally, specific treatments...competent cells. Pro-Tips Commercial competent cells range significantly in their transformation efficiency...

... antibody concentration will vary but generally ranges from 1-10 µg/mL. Add 500 µL of the diluted antibody... antibody concentration will vary but generally ranges from 1-10 µg/mL. Add 500 µL fluorescently-labeled...

...much as several miligrams and at concentrations ranging from 150 ng/μL to several μg/μL. The protocol below...pellet (can be done by inverting the tube at an angle over kimwipe) for 20-30 min. Resuspend pellet with...

...reverse primers are stable within this temperature range to anneal to each of the single stranded DNA template...polymerase that can withstand radical temperature changes during a typical PCR. The DNA polymerase has an...

.... Optional: perform a media exchange at ~18 h post transfection. Changing the media can reduce toxicity...

...measured under the 260/280 column (A good purity ranges from 1.80-2.00). Repeat for each sample. Notes:...

... streak #1. Pro-Tips Hold your tooth pick at an angle, the way you would hold a pencil, so that you can...

...note we have found that there is generally a broad range of antibiotic concentrations that will work for ...

... gels, so you will want to stay in the 0.7-0.8% range if possible. You will want nice crisp bands. This...

... bacterial endonucleases that recognize a large range of DNA sequences. Given the variety of these enzymes...

...practical lab protocols that you can use for a wide range of applications, with videos for select protocols...

...Data Figure 1: 293T cells were transduced with a range of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h ...

...contaminated with pathogens or chemicals that present a danger to people and the environment in biohazard containers...

... cells. Refer to the table below for a possible range of ratios to test: Ratio of DNA:PEI Amount of DNA...

... cells. Refer to the table below for a possible range of ratios to test: Ratio of DNA:PEI μg of DNA μL...

...your cell culture dish. Microscopes come in a huge range of shapes and sizes - from phone-sized, foldable...

... determined for your sample. Typical ratios may range from 1:1 to 1:6. Add the diluted PEI-MAX to the ...

...Prepare serial dilutions of the BSA standard that range from 0–2000 µg/mL . In duplicate, dilute 10 µL of...