...target to show a dose response. Sharing speeds science. We believe that sharing the full details of our protocols...plate. Place the plate on a microplate shaker set at 400 rpm and shake for 1 min . Repeat steps 8–10 twice...plate Place the plate on a microplate shaker set at 400 rpm and shake for 1 min . Repeat steps 18–20 twice...plate Place the plate on a microplate shaker set at 400 rpm and shake for 1 min . Repeat steps 30–32 twice...plate Place the plate on a microplate shaker set at 400 rpm and shake for 1 min . Repeat steps 40–42 twice...reservoirs Pipette controller Microcentrifuge Microplate shaker ELISA plate washer (optional) Scale Microsoft Excel... with a plate seal and incubate on a microplate shaker set to 400 rpm for 2 h at room temperature or overnight...

...SDS-PAGE gel, and immunoblotting. Sharing speeds science. We believe that sharing the full details of our protocols... at RT on a shaking platform. Wash the membrane 3x for 5 min in 1X TBST at RT on a shaking platform. Section... at RT on a shaking platform. Wash the membrane 3x for 5 min in 1X TBST at RT on a shaking platform. Prepare...iBlot 2 Gel Transfer Device Roller Spatula Platform shaker Cold room Gel imager -80 °C freezer Reagents 1X...comb. Rinse the wells with deionized water 3x . Shake the gel gently between washes to remove residual...the membrane 3x for 5 min in 1X TBST at RT on a shaking platform. Dilute the horseradish peroxidase-conjugated...

... While You Work Dispose of sharps in a designated sharps container. Sharps include anything that can pierce...protection or face shields Eye wash Safety shower Sharps container Biohazard waste container Fire blanket...including, but not limited to broken glass or needles. Sharps containers must be thick walled, impenetrable by...you may be working with a protocol that requires shaking or mixing, which may produce aerosols or splashes...Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational purposes only...

...a variety of applications. Sharing speeds science. We believe that sharing the full details of our protocols...Automated cell counter 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead bath Vortex ...flask.Incubate in a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm. Pro-Tip Do not use cells...

...cuvette. Shake four 14 mL Vented Falcon Tubes at 30-37 ℃, 225 rpm for 1 hour. After the 1 hour shaking period...restriction enzyme for digest that will cut the shared plasmid backbone a single time and visualize that...Do so by gentle spreading. Some spreaders have a sharp edge that can scrape plates more abrasively at a...

...Protein A or Protein G columns. Sharing speeds science. We believe that sharing the full details of our protocols...spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead bath Clamp stand...

...spreading; the tip is gently bent to create an “L” shape, and then used like a cell spreader. Several other...above result represents a single experiment. For publishable data, the experiment would need to be repeated...

...Incubate bacterial culture at 37°C for 12-18 hr in a shaking incubator. Note: Some plasmids or strains require...increase the density of the culture. Normally cultures shake at 150 - 250 rpm, increase this to 350 - 400 rpm...

...to isolate single bacterial colonies. Equipment Shaking incubator at 37 °C Stationary incubator at 37 °...without antibiotic) to the bacteria and grow in 37°C shaking incubator for 45 min. Pro-Tip This outgrowth step...

...cells, target proteins, etc. Sharing speeds science. We believe that sharing the full details of our protocols...

... the sample is determined. Sharing speeds science. We believe that sharing the full details of our protocols...

...mL of Chloroform/Isoamyl alcohol (49:1) and then shake vigorously by hand for 10 seconds. Incubate sample...Isoamyl alcohol (49:1) per 1 mL of TRIzol® used. Shake vigorously by hand for 10 seconds. Incubate the ...

...in the previous step. Pipette back and forth or shake vigorously to obtain a single cell suspension (no.... Add 4 mL of PEI (1:2 μg DNA to μg PEI ratio). Shake the bottle up/down vigorously for 30 sec (it’s okay...

...improve the shelf life. It is very important that you shake the glycerol before freezing (5-6 times). Make sure...

...Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational purposes only...

...loud noises or the centrifuge begins to wobble or shake, this could indicate an unbalanced or improperly...

...one reaction. However, some labs have observed a sharp decrease in success rate when assembling more than...

...culture dish. Microscopes come in a huge range of shapes and sizes - from phone-sized, foldable microscopes...

...ultracentrifugation. The steps are clearly distinguishable thanks to the use of phenol red. Image adapted...

...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-...