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Addgene
Showing: 1 - 8 of 8 results
  1. CRISPR Guide

    Type
    Guide
    ..., A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp, P. A., & Zhang, F. (2015...cleave a given locus if the gRNA spacer sequence shares sufficient homology with the target DNA. Once the... S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., Cradick, T. J., Marraffini, L. A., Bao, G..... S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, ...Garcia, B., Hidalgo-Reyes, Y., Lee, J., Edraki, A., Shah, M., Sontheimer, E. J., Maxwell, K. L., & Davidson... (18), 3983-4002.e26. PMID: 37657419 Eid, A., Alshareef, S., & Mahfouz, M. M. (2018). CRISPR base editors...Communications . 9 (11), e1007749. PMID: 30575746 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott...
  2. Chemogenetics Guide

    Type
    Guide
    ...Brown TM, Lipscombe D, Moore CI, Hochgeschwender U, Shaner NC (2024). Efficient opto- and chemogenetic control...Gomez JL, Bonaventura J, Lesniak W, Mathews WB, Sysa-Shah P, Rodriguez LA, Ellis RJ, Richie CT, Harvey BK,... Barnett LM, Lambert GG, Lipscombe D, Moore CI, Shaner NC, Hochgeschwender U (2024). Engineering luminopsins...
  3. Antibody Guide

    Type
    Guide
    ...commonly used affinity reagents. Antibodies are Y-shaped proteins, naturally produced by the immune system... These four domains come together to form a “Y” shape, as shown in Figure 1. The two arms of the Y structure...
  4. Sequencing Primers

    Type
    Guide
    ... forward primer pCasper-F GGGTTTTATTAACTTACAT (Vosshall lab) 5' end of Drosophila mini-white gene, reverse...
  5. Cloning

    Type
    Guide
    ...oligonucleotide stitching, in which pieces of DNA that share no end homology can still be fused together in a...
  6. Lentiviral Guide

    Type
    Guide
    ...indicate that the viral integrase plays a role in shaping integration site preferences. A major cellular ...
  7. Optogenetics Guide

    Type
    Guide
    ...from Haloarcula (Halobacterium) salinarum (strain Shark), used to silence neuronal activity 632 Halo/NpHR...
  8. Molecular Biology Reference

    Type
    Guide
    ...propagating plasmids E. coli are gram-negative, rod shaped bacteria naturally found in the intestinal tract...
Showing: 1 - 8 of 8 results