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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12 results for: mak

Showing: 1 - 12 of 12 results

  1. CRISPR Guide

    Type
    Guide
    ...generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR perfect... Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. ...very close proximity to the region to be edited, making the PAM sequence (NGG) limiting. This PAM is abundant...Anti-CRISPR Small Precision Edits CRISPR can be used to make small, very precise edits of a handful of bases,...RNA-binding protein to stabilize pegRNAs Twin PE — two PEs make complementary edits to opposite strands Prime editing...the ability to target almost any genomic locus, makes CRISPR an ideal genome engineering system for large-scale...large-scale, forward genetic screening. CRISPR can make highly specific, permanent genetic modifications...

  2. Molecular Biology Reference

    Type
    Guide
    ...order of nucleotides makes up the genetic code and provides the instructions to make every protein within...Once you have constructed a plasmid, you can easily make an endless number of copies of the plasmid using...of the known primer region with very few errors, making it an efficient and reliable sequencing method....and can only sequence short pieces of DNA. This makes it difficult to sequence an entire plasmid or genome...A,C,T, or G) is labeled with a different color, making it easy to identify the order of the DNA strand...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...engineered lentiviral vectors have many aspects that make them useful research tools in a variety of cell ...plasmids also contain a deletion in the 3’ LTR to make them self-inactivating (SIN). This deletion is transferred... long-term, constitutive expression. In order to make stable cell lines, VSV-G is often put under an inducible...Stable Cell Lines Lentiviral vectors can be used to make stable cell lines due to their integration into ... by daughter cells. Inheritance of the barcodes makes lentiviral libraries popular for lineage tracing...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...number will continue to increase. This diversity makes rAAV vectors a very useful system for preferentially...capacity for long-term gene expression. These features make them ideal for gene therapy, neuroscience research...channelrhodopsins, halorhodopsins, and other optogenetic genes makes them suitable to be packaged in the relatively small...choice for the delivery of CRISPR/Cas9 elements, making it one of the most common methods for in vivo CRISPR-based...shown higher efficiency in delivering large genes makes use of split inteins, proteins that carry out a ...

  5. Guide to Using Pooled Libraries

    Type
    Guide
    ...plasmid libraries, the plasmids must first be used to make virus. This pooled virus is subsequently used to...that an infected cell receives only one plasmid. To make sure that every plasmid is adequately represented...electroporation and maxiprep). If delivering as virus, make virus; this creates a pooled lentiviral CRISPR library...

  6. Chemogenetics Guide

    Type
    Guide
    ...exhibited high levels of constitutive activity, making them less than ideal. DREADDs Designer Receptors... previously used in human populations in Japan, making it an attractive option for translational studies... workflows (such as laser or LED illumination), making LMOs a versatile option. Coupling brighter bioluminescent...

  7. Optogenetics Guide

    Type
    Guide
    ... invasive. Different activation wavelengths also make it possible to combine multiple opsins in the same...196: 1-28. PMID 22341318 Gradinaru V, Zhang F, Ramakrishnan C, Mattis J, Prakash R, Diester I, Goshen I,...PMID 20621963 Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R, Gunaydin LA, Hyun M...

  8. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... gamma-retroviral vectors have many aspects that make them useful research tools in a variety of cell ... long-term, constitutive expression. In order to make stable cell lines, VSV-G is often put under an inducible...Lines Gamma-retroviral vectors are often used to make stable cell lines. Like their popular lentiviral...

  9. Antibody Guide

    Type
    Guide
    ...plasmids. Animals - Antibodies produced in animals make use of the animal’s immune system. The antigen of...Overview of application Collect and lyse samples to make proteins available. Tissue samples may need additional...Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made from plasmids...

  10. Promoters

    Type
    Guide
    ...a given gene. There are three main portions that make up a eukaryotic promoter: the core promoter, the...proteins (such as activators and structural proteins) make up the transcription complex. The most 3' portion...

  11. Molecular Cloning Techniques

    Type
    Guide
    ...repeated throughout a specific DNA sequence. This can make it difficult at times to identify compatible restriction...site-specific recombination or a ligation step, making it an easy, cheap, and rapid cloning method. LIC...

  12. Plan Your Experiment

    Type
    Guide
    ...setting up CRISPR experiments. We will focus on making single edits using CRISPR/Cas9 in mammalian cells...gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most cases gRNA...
Showing: 1 - 12 of 12 results