... different fluorescent labels. The machine detects which fluorescently labeled nucleotide is present at...combined discoveries of restriction enzymes, DNA ligase, and gel electrophoresis allowed for the ability...strands of varying lengths whose last nucleotide is labeled. Once all the newly synthesized DNA is made, the... of DNA is first fragmented into small pieces, labeled with a short DNA barcode, and amplified. These ...base is added. Again, each base (A,C,T, or G) is labelled with a different color making it easy to identify...and the machine cannot distinguish between the 4 labelled nucleotides. Epitope tags on the other hand are...

...researchers have turned dCas9 into a customizable DNA labeler compatible with fluorescence microscopy in living...genomic locus requires recruitment of many copies of labeled proteins to the given region. For example, chromosome-specific...proximity. A non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus...acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system . The locus is subsequently... . 529(7587):490-5. PMID: 26735016 Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs... 33(5):543-8. PMID: 25803306 Multicolor CRISPR labeling of chromosomal loci in human cells. 2015. Ma H...