We narrowed to 2 results for: ligase labeling

...combined discoveries of restriction enzymes, DNA ligases, and gel electrophoresis enabled the movement of...strands of varying lengths whose last nucleotide is labeled. These DNA strands are separated by size, read ... piece of DNA is fragmented into small pieces, labeled with a short DNA barcode, and amplified. These ...base is added. Again, each base (A,C,T, or G) is labeled with a different color, making it easy to identify...the machine cannot distinguish between the four labeled nucleotides, or in biologically relevant contexts...NGS, therefore does not require any “normal” (nonlabeled) bases in the reaction. All the sequenced templates...

...visualize chromatin and RNA dynamics in live cells. Labeling can be done by fusing catalytically inactive dCas9...protein (GFP), creating a customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like ...genomic locus requires recruitment of many copies of labeled proteins to the site. Chromosome-specific repetitive...proximity. A non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus...RNA in live cells, including orthogonal RNA/DNA labeling in the same cell. Figure 11: Overview of visualizing...acceptor site to dCas9 and co-expressing BirA biotin ligase. Whatever the chosen epitope tag, the locus is ..., S., & Pederson, T. (2015). Multicolor CRISPR labeling of chromosomal loci in human cells. PNAS . 112...