Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 5 results for: codon optimized

Showing: 1 - 5 of 5 results

  1. Molecular Biology Reference

    Type
    Guide
    ...termed a codon. There are 64 codon combinations and only 20 natural amino acids, so multiple codons can encode...multiple codons code for each amino acid. There are 20 amino acids plus a start and stop codon. Below you...ochre) *AUG is the most common start codon. Alternative start codons include CUG in eukaryotes and GUG in...degeneracy. Refer to the amino acid table below for codon-amino acid pairs. Over the past two decades, researchers...also expanded the genetic code by redirecting some codons to encode for synthetic amino acids. Plasmids and...of replication, and an MCS. They are small and optimized to help in the initial cloning of a DNA fragment...field of recombinant DNA technology have been optimized for studying and manipulating genes. For instance...

  2. Promoters

    Type
    Guide
    ...The coding strand is the DNA strand that encodes codons and whose sequence corresponds to the mRNA transcript...promoter region is located closest to the start codon and contains the RNA polymerase binding site, TATA...The most 3' portion (closest to the gene's start codon) of the core promoter is the TSS, which is where...Tetracycline response element promoter; often optimized for mammalian systems Types of RNA Polymerases...

  3. Optogenetics Guide

    Type
    Guide
    ...been created via genetic point mutations, codon optimization, and chimeric fusion of domains from two ...engineered with enhancements such as: Human codon optimization Example: Halo Increased photocurrent amplitude...

  4. CRISPR Guide

    Type
    Guide
    ... an optimized gRNA for your application (see: gRNA Design Software ). In addition to an optimized gRNA... frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the targeted...structured motif to enhance pegRNA stability PEmax — optimized PE architecture; increased synergy between PE ...compared to HDR. Prime editing requires more optimization and typically has lower efficiency (< 50%) than.... gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high on-target activity...shifting the ORF and/or creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA repair...sequence ORF O pen R eading F rame; The translated codons that make up a gene PAM P rotospacer A djacent ...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...different retroviral vectors using a novel, CoDOn-Optimized gene for chimeric GALV envelope. Viruses , ...
Showing: 1 - 5 of 5 results