...Description 41824 gRNA Empty Vector The backbone that a target sequence gets cloned into to create the gRNA...protocol for synthesizing gRNAs: Cas9 orthologs gRNA cloning protocol 104.1 KB This set of plasmids is described...tracrRNA expression, cloDF13/spectinomycin 48649 PM-SP!TA Bacterial SP crRNA expression: targets SP to protospacer...ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48651 PM-NM!TA Bacterial NM crRNA expression: targets NM to protospacer...ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48653 PM-ST1!TA Bacterial ST1 crRNA expression: targets ST1 to protospacer...ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48655 PM-TD!TA Bacterial TD crRNA expression: targets TD to protospacer...