Skip to main content
Addgene
Showing: 1 - 20 of 37 results

  1. Genetic Code Expansion

    Type
    Collection
    ...Mammalian TAA Simon Elsaesser 157925 pMAH-POLY-eRF1(E55D) POLY-eRF1(E55D) E. coli para-substituted phenylalanine...azidonorleucine (Anl) Mammalian ATG David Tirrell 64915 pMAH-POLY tyrosyl-tRNA synthetase E. coli pBof Mammalian TAG...proteins for various purposes from therapeutics to biopolymers. Tips for Success Before beginning to reprogram...

  2. Tetracycline Inducible Expression

    Type
    Collection
    ...Expression of your gene of interest (MCS with a β-globin poly A) & EGFP from a bidirectional tet-responsive promoter...

  3. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Bradley 3rd ~9 pairs/exon 9,508 Mouse CRISPR Proximal Poly(A) Site Knockout Library 190744 Knockout Mouse Bradley...

  4. Open Enzyme Collection

    Type
    Collection
    ... DNA Polymerase I 165533 pOpen-TthpolA Tth DNA polymerase 165534 pOpen-T7gene5 T7 DNA Polymerase (unmodified... Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes... of molecular biology, including DNA polymerases, RNA polymerases, DNA ligases, reverse transcriptases... DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N-7 DNA polymerase 165501.... Heat-Stable DNA Polymerase 165502 pOpen-9N7polA (CT) 9 degrees N-7 DNA Polymerase chain terminating ...pOpen-K12polLF DNA Polymerase I, Large (Klenow) Fragment 165522 pOpen-K12polLF (exo-) DNA Polymerase I, Large (...

  5. Immunology Research Plasmids and Resources

    Type
    Collection
    ...class I polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related...catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase, catalytic, gamma polypeptide PI3CG, PI3K...gonadotropin, beta polypeptide CGB3, hCGB CGB1 chorionic gonadotropin, beta polypeptide 1 - CGB2 chorionic... gonadotropin, beta polypeptide 2 - CGB5 chorionic gonadotropin, beta polypeptide 5 HCG, MGC119822 CGB7... beta polypeptide 7 CG-beta-a, FLJ35403, FLJ43118 CGB8 chorionic gonadotropin, beta polypeptide 8 - CHGA... - GIP gastric inhibitory polypeptide - GIPR gastric inhibitory polypeptide receptor MGC126722 GKN1 gastrokine...factor alpha polypeptide PDGF-A, PDGF1 PDGFB platelet-derived growth factor beta polypeptide (simian sarcoma...

  6. Plasmids for Stem Cell Research

    Type
    Collection
    ...Single polycistronic lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral...27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral vector for the expression of human...(8):1221-9. Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for...Non-integrating mini-intronic plasmid (MIP) polycistronic expression of human Oct4, Klf4, Sox2, c-Myc ...episome Human Non-integrating EBNA1-mediated polycistronic expression of human Sox2, KLF4, L-Myc, Lin28...Replicating EBNA1 episome Human Non-integrating polycistronic expression of human Oct4, Klf4, Sox2, c-Myc,...episome Human Non-integrating EBNA1-mediated polycistronic expression of human Oct4, Sox2, Myc, Klf4, BCL2L1...

  7. Antibody Guide

    Type
    Collection
    ...lower background signals than polyclonal antibodies. However, polyclonal antibodies are more sensitive...clone that are specific to a certain epitope, or polyclonal, meaning there are many clones that are specific... antibodies are typically more expensive than polyclonal antibodies. Environmental factors, binding partners...available epitope on your protein of interest. Polyclonal antibodies are typically collected directly from...targeting various epitopes on the same antigen. Polyclonal antibodies are often more environmentally stable...protein in any form it may be present in. However, polyclonals can vary significantly from lot to lot, as immune...consider the following questions: Do you need polyclonal or monoclonal antibodies? Will you be using a...

  8. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ... PCR and surveyor. I used DNA polymerase Takara Ex Taq ™ DNA Polymerase for my genomic PCR, but couldn't...In our hands, Herculase II Fusion polymerase or Kapa Hifi Polymerase work very well. Some groups have ...your HR by doing Restriction Fragment Length Polymorphism (RFLP) ( see Figure 4 of the Cong, et. al, 2013...

  9. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Marchantia polymorpha MpU6 none S. pyogenes Hara-Nishimura pMpGE_En03 71535 Other/Marchantia polymorpha MpU6...pyogenes Ventura pCRISPRyl 70007 Other/Yarrowia lipolytica SCR1'-tRNA yes, cut S. pyogenes LEU2 Wheeldon...pyogenes Draetta pCAS1yl 73226 Other/Yarrowia lipolytica yes, cut S. pyogenes LEU2 Yang pLCKO 73311 Mammalian...Gateway recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with up to 3 ...in a single vector. sgRNAs are expressed as polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG ...

  10. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Ligation Buffer, 6.5 μl ddH 2 O, and 0.5 μl T4 Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated...acceptable. The bulk sorted cells are composed of a polyclonal population exposed to sgRNA-A and sgRNA-B (see... band” in separate reactions. NOTE: Numerous polymerases may be used for step 6.3 . Run samples in a thermocycler...a single reaction. Optimize multiplexing in a polyclonal population ( i.e., bulk sorted cells) before ...biallelic deletion clones with a proofreading polymerase and clone the amplicons ( e.g., with a PCR cloning...

  11. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells Kotin 65214...production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells Kotin 65215...production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells Kotin 65216...production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells Kotin 92307...

  12. Viral Production

    Type
    Collection
    ...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...centrifugation and then by filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then ... 293T cell line in the presence of 10.0 μg/mL polybrene. This ensures that any loss of titer associated...

  13. Microbiology Resources

    Type
    Collection
    ...vaccinia virus - Bennink and Yewdell Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly...transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors...

  15. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Adeno X shuttle vector with EF1 promoter and SV40 polyA pICPIS-CB 73356 AdenoX shuttle vector with chicken-beta...chicken-beta actin CMV enhancer promoter SV40 polyA...

  16. COVID-19 Resources

    Type
    Collection
    ...window) reverse transcriptase, protease, and Bst polymerase constructs now available from Andrea Pauli's ...Diagnostic Toolkit Enzymes. (unpublished) Taq polymerase and MMLV reverse transcriptase expression plasmids...

  17. Bacterial Expression Systems

    Type
    Collection
    ...with a 6x His tag. Requires a strain with T7 RNA polymerase. Amp resistant. More expression vectors for protein... of the pB1H2w derivatives as a fusion to RNA polymerase subunit ω. Transcription factor binding to a ...

  18. Luciferase Plasmid Collection

    Type
    Collection
    ...Michael Rosbash 128046 pGL3basic luciferase and renilla_polyA Firefly/Renilla Insertion of 5' promoter/enhancer...luciferase James Wilson 101156 T7-CMVtrans-FFLuc-polyA Firefly T7 Expression of firefly luciferase from...

  19. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mRFP-Clc Clathrin-coated vesicles Clathrin, light polypeptide mRFP Ari Helenius 70217 pEGFP-GFP11-Clathrin ...Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437 mRFP-Rab5 Early endosomes...

  20. Cre-lox system

    Type
    Collection
    ... metallothionein MT-I region including the polyadenylation site and several introns tet-hCMV Mammalian...Yang 107313 AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA mCherry and Cre; expressed in excitatory neurons...
Showing: 1 - 20 of 37 results