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  1. CRISPR References and Information

    Type
    Collection
    ...pAc-sgRNA-Cas9 PDF 165.1 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF 125.6 KB Marraffini Bacteria: pCRISPR...efficiency. DeepSpCas9 (Link opens in a new window) : a webtool that provides models for SpCas9 gRNA design...Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Link O'Connor-Giles Fly: gRNA... for Genomic Deletions in Mammalian Cell Lines pSpCas9(BB) (pX330) Link Parrott NEBuilder Assembly of ...

  2. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Kanamycin none, need Cas9 plasmid Marraffini 42876 pCas9 BsaI E. coli, S. pneumoniae Chloramphenicol yes,...

  3. Bacterial Expression Systems

    Type
    Collection
    ... your research. Plasmid ID Technique PI Purpose pCas9 42876 CRISPR Luciano Marraffini Bacterial expression...

  4. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...BsaI none S. pyogenes Chloramphenicol Marraffini pCas9 42876 Bacteria BsaI yes, cut S. pyogenes Chloramphenicol...backbone 61424 Mammalian BbsI none S. pyogenes Zhang pSpCas9n(BB)-2A-Puro (PX462) V2.0 62987 Mammalian BbsI ...BbsI yes, nick S. pyogenes Puro Zhang pSpCas9(BB)-2A-Puro (PX459) V2.0 62988 Mammalian BbsI yes, cut S. pyogenes... pyogenes Puro Zoldos pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110) 71707 Mammalian hU6 yes, cut S. pyogenes...-crRNA 78959 Mammalian U6 none Mb Cpf1 Kim pCAG-SpCas9-GFP-U6-gRNA 79144 Mammalian hU6 yes, cut S. pyogenes...Yeast RPR1(TetO) yes, cut S. pyogenes URA3 Davis eSpCas9(1.1) 71814 Mammalian U6 yes, cut (enhanced) S. ...

  5. Zhang Lab CRISPR Page

    Type
    Collection
    ...efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A nickase...plasmid expressing SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase...expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and the single guide RNA. The vector can ... 3. SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9; sgRNA...codon-optimized SpCas9 plasmids can be ordered via the links below: 42230 : PX330; SpCas9 and single guide...guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro and single guide...RNA 48873 : PX460; SpCas9n (D10A nickase) and single guide RNA 48140 : PX461; SpCas9n-2A-EGFP (D10A nickase...

  6. CRISPR Guide

    Type
    Collection
    ...pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' NGG (reduced NAG binding) SpCas9 VRER variant 3'...NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3...engineered Cas9s can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the most popular...no cuts. Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain and one inactivated...fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) - weaken interactions between the HNH/RuvC...RuvC groove and the non-target DNA strand SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone...your desired genes for modification. Engineered SpCas9 enzymes with altered PAM specificities are available...

  7. Lentiviral Prep Service

    Type
    Collection
    ...-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting 19,114 human...non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs targeting 19,114 human genes...non-targeting controls. This backbone does not contain SpCas9. 78,637 unique sgRNAs targeting 19,674 mouse genes...

  8. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very low off-target...Researchers have expanded the CRISPR field beyond SpCas9, moving us closer to being able to target every... Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs...

  9. CRISPR Plasmids - Tagging

    Type
    Collection
    ... AAVS1 genomic safe harbor locus using untagged SpCas9 (Addgene 41815 or #44719 ) in combination with ...using an all in one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which ... integrating at the AAVS1 "safe harbor" locus: eSpCas9(1.1)_No_FLAG_AAVS1_T2 Doyon Lab TAP Tagging Protocol...

  10. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...purposes. NOTE: Here we discuss the usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230)....allows for the simultaneous expression of sgRNA and SpCas9, but does not contain markers for selection 1 ....

  12. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are upstream of ...

  13. Plant Plasmids and Resources

    Type
    Collection
    ...Kit includes a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an intronized Cas9 series...

  14. Validated gRNA Sequences

    Type
    Collection
    ... or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be used with targets that are upstream of ...
Showing: 1 - 15 of 15 results