...visualize chromatin and RNA dynamics in live cells. Labeling can be done by fusing catalytically inactive dCas9...protein (GFP), creating a customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like ...genomic locus requires recruitment of many copies of labeled proteins to the site. Chromosome-specific repetitive...proximity. A non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus...RNA in live cells, including orthogonal RNA/DNA labeling in the same cell. Figure 11: Overview of visualizing...acceptor site to dCas9 and co-expressing BirA biotin ligase. Whatever the chosen epitope tag, the locus is ..., S., & Pederson, T. (2015). Multicolor CRISPR labeling of chromosomal loci in human cells. PNAS . 112...