...cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors for cancer...PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome editing...constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9 system ...sequence (20bp). CRISPR-Cas9 Cre expression vectors for genome editing in the brain Using Cas9 mice Platt et...pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in neurons. SpCas9 guide acceptor: The PX552 plasmid (#60958)...Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR...237.2 KB SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the ...

...the multi-plasmid SAM system. Select Expression System To use CRISPR, you will need both Cas9 and a gRNA...and/or Cas9 expression or (2) a lack of efficient target cleavage in cells expressing both Cas9 and gRNA... started using CRISPR in your research. Although we will use the example of CRISPR/Cas9 in mammalian cells...for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in... or electroporation Transient expression of CRISPR components Expression decreases as RNA is degraded ... specific plasmid in question (see CRISPR protocols from Addgene depositors ). Deliver Cas9 and gRNA Choose... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...

... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs... insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John Calarco's...elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that... genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ... Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm...Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR resources ...

...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...none, need Cas9 plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem 47912...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator... CRISPR C. elegans CRISPR Plasmids: C. ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...following CRISPR plasmids have been designed for use in C. elegans. Cut Fully functional CRISPR/Cas enzymes...need Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid...

...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin..., need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR...

...introduction to CRISPR technology or the Plant CRISPR Resources for a full selection of plasmids expressing different... Plasmid Collections Plant Plasmids Plant Plasmids and Resources...biology - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general...creating plant expression vectors: A modular plasmid assembly kit for multigene expression, gene silencing...like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription...addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use ...includes plasmids containing promoters that drive general and species-specific gene expression (see the...

... CRISPR Qi Lab CRISPR Plasmids Qi Labs CRISPR Plasmids...codon-optimized Cas9-BFP fusion expression plasmid 44248 pgRNA-humanized A customizable gRNA expression plasmid...CRISPR CRISPRi Cas9 gRNA plasmids from the Qi lab CRISPR... inactive, human codon-optimized Cas9 expression plasmid 44247 pdCas9::BFP-humanized A catalytically inactive...codon-optimized Cas9 constructs 44249 pdCas9-bacteria A catalytically inactive bacterial Cas9 expression plasmid...plasmid 44250 pwtCas9-bacteria A wild-type Cas9 expression plasmid for use editing bacterial genomes 44251...customizable gRNA expression plasmid for use with the bacterial Cas9 constructs 46910 pHR-SFFV-dCas9-BFP Human...

...human cells CRISPR-Cas/RGN expression plasmids for use in zebrafish Guide RNA expression plasmids for the ...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR Protocols...specificities Dimeric CRISPR RNA-guided FokI Nucleases (RFNs) CRISPR-Cas/RGN expression plasmids for use in human...Gene Guide RNA expression plasmids for endogenous human genes Guide RNA expression plasmids for endogenous...of Staphylococcus aureus Cas9 (SaCas9 KKH variant) Engineered CRISPR-Cas9 nucleases with altered PAM specificities...pages describing Joung Lab CRISPR-Cas/RGN reagents: Cpf1 expression plasmids (AsCpf1 and LbCpf1) for use...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids Genome...

... cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335...ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF 488.6 KB Sabatini and...Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression: pAC2..... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts In collaboration with the labs who...deposited CRISPR plasmids , we've created a series of links and guides to help you use CRISPR in your lab...KB Church gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF 104.1 KB Chen and Wente Zebrafish...design and cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning...

...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...genomic regions using CRISPR Browse Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S....References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and...many CRISPR epigenetic tools require constitutive expression of the dCas9 fusion and/or expression of multiple...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening... containing the CRISPR plasmid will have all desired genomic edits carried out by Cas9. Multiplexing applications...more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease...

... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR FAQs Zhang...Zhang Lab's CRISPR Frequently Asked Questions You may also like... Zhang Lab CRISPR plasmids General CRISPR...high expression, so adding this G could help with expression, though it is possible for the plasmid to ...double nickase, you could express two spacers and use PX335 to express the Cas9n (nickase). The concept ...need to have four spacers co-expressed and using PX335 to express the Cas9n (nickase). Homologous Recombination...using the lab's CRISPR technology. Visit the Zhang Lab page and forum to learn more. CRISPR Design FAQs Homologous...the Bench FAQs CRISPR Design FAQs Should I use wildtype or double nickase for my CRISPR genome engineering...

...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing... CRISPR Xenopus CRISPR Plasmids: Xenopus Browse...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... following CRISPR plasmids have been designed for use in Xenopus. Cut Fully functional Cas9 enzymes designed...transcription none, need Cas9 plasmid Chen Do you have suggestions for other plasmids that should be added...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...

...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert... CRISPR Bacteria CRISPR Plasmids: Bacteria...following CRISPR plasmids have been designed for use in bacteria. Cut Fully functional CRISPR/Cas enzymes...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...

...Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI pCRISPR 42875...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut...Whether the plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that can ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted...from our collection. Plasmids Expression System Description PI Multiplex CRISPR/Cas9 Assembly System Kit...

... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...backbone. Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused...Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits,...Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...

...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...following CRISPR plasmids have been designed for use in zebrafish. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...Zebrafish Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

...Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith... or the zebrafish CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones...Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector ...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...biosensors, and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description...

...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert... CRISPR Yeast CRISPR Plasmids: Yeast Browse ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... following CRISPR plasmids have been designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes...cerevisiae LEU2 none, need Cas9 plasmid Lu Do you have suggestions for other plasmids that should be added ...

...tables below for CRISPR base editing plasmids. Plasmids are available for expression in mammalian systems...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two...are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR...editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have ...(TAM) from the Chang lab and CRISPR-X from the Bassik lab , in which Cas9 is fused to activation-induced...

...Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...proteins. This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt...Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged Cas9 AND a gRNA...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...