...Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal ...Glucose Green fluorescent glucose indicators for living cells (Glifons) Green Fluorescent Protein-Based ...Lactate Lindoblum green fluorescent biosensor for lactate Green fluorescent protein-based lactate and ...Pyruvate Pegassos green fluorescent biosensor for pyruvate Green fluorescent protein-based lactate and...cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP... AAV Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties are ... from a nested cassette of green- and orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1...

...Michael Davidson Fluorescent Protein Collection Michael Davidson Fluorescent Protein Collection More Fluorescent...lab fluorescent protein fusion constructs and backbones. Plasmid... Plasmid Collections Fluorescent Proteins Michael...collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs are of mammalian...empty backbone with different fluorescent tags for you to create fusion proteins with your gene of interest...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The lab of Michael...Empty Backbones mEmeralds Reds Blues Cyans Oranges Greens Yellows Photoactivatables Empty Backbones Plasmid...

...Special Collections Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones...extensively by life scientists. The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et ...Expression mAzamiGreen 492 505 41 5.8 Monomer mAzami Green-N1 (mAG) - Mammalian Expression mAzami Green-C1 (... backbones with different fluorescent tags for you to create fusion proteins with your gene of interest...Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson Collection... Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a Monomer...Monomer not a Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively...

...Photoconvertible , and Photoswitchable fluorescent proteins Various PIs Fluorescent protein fusion: contains empty vectors...and C-terminal fluorescent protein tags that can be used to create fluorescent protein fusions with your...mEos2 20341 mEos2 (fluorescent protein) Loren Looger Photoactivable Fluorescent Protein Expression: contains...Empty Backbones with Fluorescent protein tags Various Plasmids Blue/UV , Cyan , Green , Yellow , Orange ...your protein of interest. See an overview of Addgene fluorescent protein resources here . pCyPet-His 14030...cyan fluorescent protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent protein...yellow fluorescent protein variant (YPet) optimized for FRET measurements with a cyan fluorescent protein...

...overexpress various fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast....create fusions of proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are... Lim WA, Thorn KS. Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources FP Tagging FP Overexpression...comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources External Resources Teusink..., Teusink B. In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep. 2019 Feb 19;9...

...expressed in the retina). Fluorescent Cre: The fusion of Cre to a fluorescent reporter enables visualization...pCMV-cre-K Cre-K CMV Mammalian Green 8395 p210 pCMV-CREM CREM CMV Mammalian Green 8401 p224 pCMV-RFP/CREM floxed...within CREM CMV Mammalian Green 8403 p153 pPr-cre Cre probasin Mammalian Green 8404 p236 pPr-CREM CREM ...probasin Mammalian Green 8405 p240 pPr-CREM/Ins CREM with insulators probasin Mammalian Green 8406 p252 pPr-CREM...Mammalian Green 12494 p260 pCMV-CRE-M-AR(wt) CREM fused to AR ligand binding domain CMV Mammalian Green 12496... a stochastic choice of expression among fluorescent proteins, resulting in the indelible marking of mouse...specific system for controlling gene expression. The protein Cre recombinase recognizes 34 bp loxP sites, and...

...Collections Fluorescent Proteins FRET Fluorescent Protein Guide: FRET More Fluorescent Protein Resources...for creating individual fluorescently tagged proteins to study protein-protein interactions and a series...not required to be fluorescent. A variety of organic dyes and fluorescent proteins have been developed...is often used to study (1) protein-protein interactions where each protein is separately fused to a donor...Encoding Fluorescent Proteins for FRET The following plasmids can be used to create a fluorescent fusion...fusion protein with your gene of interest and the listed fluorescent protein. Plasmid Color Expression Description...changes within a protein where the donor and acceptor are both fused to the same protein (also referred ...

...Browse our control AAV collection, including fluorescent proteins, spaghetti monster, and more.... AAV Browse our control AAV that express fluorescent proteins under general promoters. These AAV can be...and variants Synapsin TBG Other Fluorophore/Tag Green Red None Other Cre-dependent color switch Brainbow...pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 CAG GFPsm_myc (does not fluoresce) Cre dependent 1 Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 CAG Ruby2sm-Flag (does not fluoresce) Cre dependent 1 Looger 45185 AAV-EF1a-BbTagBY...dependent 1 Jensen 99133 pAAV-CAG-fDIO-mNeonGreen CAG mNeonGreen Flp dependent 1 Gradinaru 137131 pAAV-...

... Plasmid Collections Fluorescent Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization...marker protein is fused to GFP, you may want to fuse your protein to a red fluorescent protein (see this...structures. You should fuse your protein of interest with a fluorescent protein that has a different emission...your fusion protein and the marker fusion protein using fluorescence microscopy, you can assess whether...Targeting Gene/Signal Fluorescent Protein PI 98875 3xnls-mNeonGreen Nucleus 3xNLS mNeonGreen Dorus Gadella 98817...Plasmids encoding fluorescent proteins tagged with genes or peptides with known subcellular localization...Localization More Fluorescent Protein Resources: Empty Backbones Allen Institute for Cell Science Plasmid Collection...

...co-immunoprecipitation to assess protein-protein interactions. NanoLuc® complementation : Protein-protein assays based on... Plasmid Collection You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies ...-learning approaches. Split-Luciferase and Protein-Protein Interaction Assays Cluc-FKBP , FRB-Nluc : Split...mmunoprecipitation) plasmids : Luminescence-based protein-protein screening method for the systematic and quantitative...NanoLuc® have been used to assess GPCR-G protein interactions , protein dimerization , endosomal escape of ..., Enhanced Nano-lanterns , and Antares : Fluorescent proteins are fused to Renilla luciferase (Nano-lanterns...include Gaussia luciferase (Gluc) and the red and green Click-beetle luciferases (CBRluc and CBGluc, respectively...

... Special Collections Fluorescent Protein Guide Fluorescent Protein Plasmids & Resources You May...Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Fluorescent Protein Kits Blog: When is a... variety of fluorescent proteins, as well as over 100 empty backbones. Fluorescent Protein Kits cpFRET...and/or function of your protein of interest by fusing it to a fluorescent protein. FRET Find FRET pairs ...standards to study protein-protein interactions or conformational changes within a protein. Biosensors Monitor...where your protein of interest resides by using a well-characterized fluorescent fusion protein. In Vivo...guide to learn about the applications of fluorescent proteins and to find the plasmids that are available...

...encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru...AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru...hSyn1-eYFP An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter Viviana Gradinaru...

...Return to top Fluorescent Proteins The table below highlights plasmids that feature fluorescent or luminescent... may also like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR ...Transcription Factors RNAi Empty Backbones Fluorescent Proteins Other Resources Background Addgene’s repository...genome engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids ... such as the promoter or fluorescent protein of choice. ID Plasmid Description Promoter PI Return to top...Marillonnet and Johannes Stuttmann. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly...luminescent proteins for expression in plants. You can search the table for features you are looking for, such...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...; or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can... sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to...

... and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant...Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination...

...Biosensor AAV Browse our AAV that express fluorescent biosensor proteins, including calcium sensors, under the... the control of various promoters. Sensor Green Calcium Sensors GCaMP8 axon-GCaMP8 soma-GCaMP8 ribo-GCaMP8...

...plasmid type, mutations, and/or tags such as fluorescent protein (FP) tags. Information on genes and associated...Omi-mCherry HTRA2 mCherry LTR Parkinson's Douglas Green 50487 pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos...Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139125 hnRNPF_PLD_StoA HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Erik Roberson 179896 pMKRQ_11_DExCon-mNeonGreen-R25 RAB25 mNeonGreen TRE3GS Parkinson's Patrick Caswell ...Parkinson's Randy Schekman 205713 mNeonGreen-alpha-synuclein SNCA mNeonGreen CMV Parkinson's Randy Schekman...