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  1. FlyCRISPR

    Type
    Collection
    .... Contains the visible marker 3xP3-DsRed. (Also known as pHD-DsRed-att). 51026 U6-BbsI-crRNA : Generates...for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter. 51019 pDsRed-attP...for use in combination with tracrRNA. 51434 pHD-DsRed : vector for generating dsDNA donors for homology-directed...homology-directed repair. Contains the visible marker 3xP3-DsRed....

  2. Retrovirus Plasmids

    Type
    Collection
    ...transgene expression; also expresses luciferase Lowe 9044 pMIG MSCV Plasmid for transgene expression; also...Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope...GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for Puro resistance... and gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-... the rapid loss of dsRed and the activation of your gene fused to eGFP expression Clevers 18760 MSCV IRES...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for additional...pLncEXP CMV/MSV lncRNA expression plasmid Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian...

  3. Cre-lox system

    Type
    Collection
    ...diversity Mammalian Megason 13769 pCALNL-DsRed Cre dependent DsRed expression Mammalian Cepko 13770 pCALNL-GFP...present. By expressing Cre at specific times or locations, you can precisely control expression of your gene...developed ways to tightly control Cre expression and to optimize Cre expression once it is induced. Addgene has...defines the areas in which Cre will be expressed. Cre may be expressed globally under a broadly active promoter... or expressed only in a subset of cells under a more specific promoter (e.g. Rho-Cre is expressed in the...visualization of Cre expression. Optimized Cre: Codon-optimized Cre ( iCre ) is expressed at higher levels... EGFP, and sgRNA expression hSyn AAV Zhang 60820 pSECC Cre, Cas9 and sgRNA expression EF-1 alpha Lentiviral...

  4. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...pAdx-CMV-YFP 73348 Express YFP with an NLS from the CMV promoter pAdx-CMV-dsRed 73349 Expresses dsRed from the ...-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector pCDH-EF1...pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 promoter when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express iCre under ...pCDH-CB-FLPe-P2A-tdTomato 72259 Expresses FLPe and tdTomato from the CB promoter pCDH-EF1-Fon-copGFP 72260 Expresses copGFP from...promoter when FLP is expressed by another vector pCDH-EF1-Fon-tdTomato 72261 Expresses tdTomato from the ... promoter when FLP is expressed by another vector pCDH-EF1-FLPe 72262 Expresses FLPe from the EF-1 promoter...

  5. CRISPR References and Information

    Type
    Collection
    ...neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (pSIR-hCD2) PDF 105.8 KB... sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92...design and cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning...donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF 583.3 KB Zhang gRNA cloning CRISPR RNA...

  6. Bacterial Expression Systems

    Type
    Collection
    ... to Top Controlled Expression It isn’t always beneficial to constitutively express your gene of interest... on expression only at a specific time. This collection has a variety of plasmids whose expression levels... bacterial expression systems to find out more! Genome Engineering Although overexpressing or knocking...plasmids used for expression in bacterial systems Plasmid... Plasmid Collections Bacterial Expression Systems Bacterial...Bacterial Expression Systems Genome Engineering Visualization and Tagging Purification Controlled Expression...Tagging and Visualization Purification Controlled Expression Reporter Plasmids You may also like… Plasmids...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...pEiLOV-N1 - Mammalian Expression pGEX iLOV - Bacterial Expression iLOV-N1 - Mammalian Expression iLOV-C1 - Mammalian...Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ... Mammalian Expression mTopaz-C1 - Mammalian Expression mTopaz-pBAD - Bacterial Expression mVenus 515 528... N1 - Mammalian Expression mVenus C1 - Mammalian Expression pKT0090 - Yeast Expression mVenus-pBAD - Bacterial...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity... Huebener , Tobias Rose Calcium Soma-targeted expression of jGCaMP8f, GCaMP7f, or GCaMP6f Precision Calcium...2014 Jonathan Ting Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or...Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of GCaMP6f in PV cortical interneurons under ...-1636. Jordane Dimidschstein Calcium Enriched expression of GCaMP6s or GCaMP6f in axons (Constitutive ...Neurosci. 2018 Sep;21(9):1272-1280. Lin Tian Calcium Expression of GCaMP6s or GCaMP7b sensors fused to synaptophysin... Aug 4;11(1):3881. Adam Cohen Calcium GCaMP6f expression in forebrain GABA-ergic interneurons under the...
Showing: 1 - 8 of 8 results