... Degrading DNA with Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand...recruited to target DNA using a gRNA and recruits Cas3. Cas3 nicks the non-targeting strand and starts degrading... strand is cleaved non-specifically by Cas3 along the way. Cas3 initiates a final DSB, either due to a.... Figure 14: Overview of editing with Cas3 Browse Plasmids: Cas3 Want more information on the wide variety...Purify Genomic Regions Cas9 Alternatives Cas12a Cas13 Cas3 Resources Plan Your Experiment Web References PAM...such as AcrVA3.1, which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a ... enzymes that form a complex called Cascade (see Cas3 for more details), while type V-K systems use a ...

...) Class 2 (Single multi-domain effector) Type I (Cas3) Type II (Cas9) Type III (Cas10) Type V (Cpf1) Type...