...for: a wide range of DNA edits.   Cas3 – The shredder What sets Cas3 apart from Cas9 and almost all other...DNA break, Cas3 locates its target sequence and initiates single stranded DNA degradation. Cas3 DNA digestion...to multiple kilobases, but only in one direction. Cas3 is thus not helpful for designer genome edits, but...

...large genome edits using transposases or the Cascade-Cas3 system. We also describe newly engineered Cas9s ...

... treatment currently in clinical trials includes Cas3 (the shredder member of the Cas family) as part ...cocktail therapy for urinary tract infections. The Cas3 enzymes target several strains of E. coli and lethally...

...from gut. While still far from the clinic, CRISPR-Cas3 systems have been used successfully in mice for ...Clostridioides difficile Using Phage-Delivered CRISPR-Cas3 Antimicrobials. mBio 11: . https://doi.org/10.1128...

...interact with CRISPR-Cas systems such as Cascade-Cas3, Cas9, Cas12, and Cas13 (Marino et al., 2020). Some...

...VchINT does not recruit other nucleases (such as Cas3) or have its own nuclease activity. Instead, it ...