Skip to main content
Addgene
Showing: 81 - 100 of 135 results

  1. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...homology to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID...

  2. TALEN Guide

    Type
    Collection
    ...nuclease, the components of the array recognize individual nucleotides. For over ten years, zinc finger ...thymine, and NN targets guanine (though NN can also bind adenenine with lower specificity). With the template...also hosts web-based software to help scientists find potential TAL effector targets. The site, which ...

  3. Viral Production

    Type
    Collection
    ...of Pennsylvania Vector Core (Link opens in a new window) . Transfections are performed using the transfer...modified from Lock et al. (2014) (Link opens in a new window) ). Previous experience suggests that titers obtained...modified from Wang et al. (2018) (Link opens in a new window) ). Briefly, 293T cells are transduced with serial...

  4. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...have shown that engineered TALENs can be used to induce targeted mutations with high efficiency in zebrafish...TAAACGCCCTGATCTTCAgcgccgacggcctgcagcAGAGAGACAAGATGCGCA inhibitor of DNA binding 3 TAL3466 & TAL3467 TCAACATTGGATATCTATagagctttacagctagTTTATCCCAGTGTCCAGA...TGCAGCCGTGTTTCTACCcggacgaggatgacttCTACTTCTGCAGACCAGA NADPH oxidase, EF-hand calcium binding domain 5 TAL3496 & TAL3497 TGCCGCCAACTGGCTGAAacctccagatctggagCAGAACAAGCGCAAAA...

  5. The Pleiades Promoter Project

    Type
    Collection
    ... Pleiades Promoter Project (Link opens in a new window) was established to advance research and therapies...construct and can be accessed using the links to view individual plasmid webpages. These plasmids are described....1009158107. PubMed 20807748 (Link opens in a new window)...

  6. Biosensor AAV Preps

    Type
    Collection
    ...Constitutive 1, 5 GENIE 218873 pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE GFAP iGABASnFR2 (control) none Constitutive...Constitutive 1, 5 GENIE 218876 pGP-AAV-syn-iGABASnFR2(no bind)-WPRE Syn iGABASnFR2 (control) none Constitutive...1, 5 GENIE 218879 pGP-AAV-syn-flex-iGABASnFR2(no bind)-WPRE Syn iGABASnFR2 (control) none Cre dependent...

  7. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...of Pennsylvania Vector Core (Link opens in a new window) (Penn Vector Core) and Addgene have partnered ...discontinued. The following viral vectors in the indicated serotypes are no longer available as aliquots.... below. Please let us know (Link opens in a new window) what you would like to see available in viral ...

  8. Tags and Other Markers

    Type
    Collection
    ...Find recombinant antibodies targeting epitope tags and common fusion proteins....Antibody Collection Browse or search the table below to find antibodies currently available from our catalog ...

  9. Neuroscience

    Type
    Collection
    ...Find recombinant antibodies targeting neuroscience-related proteins....Antibody Collection Browse or search the table below to find antibodies currently available from our catalog ...

  10. Genetic Code Expansion

    Type
    Collection
    ...the ribosome and if the anticodon on the tRNA can bind to the mRNA (hence, the anticodon is complementary...the activity of a protein (e.g. converting a DNA binding protein to a DNA cleaving enzyme) or to regulate... orthogonal tRNA. And the orthogonal tRNA has to bind to an unallocated codon. Therefore many controls...

  11. Validated gRNA Sequences

    Type
    Collection
    ...us by users (see below for more details). Keep in mind that additional factors must be considered before...only be compatible with a single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be ... Plasmids and Resources page have been used to indicate the Cas9 application the gRNA was designed to ...

  12. KLF Research Plasmids

    Type
    Collection
    ...factors (KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse roles in...three C-terminal Krüppel-like zinc fingers which bind to CACCC elements or GC-rich regions on DNA to mediate...

  13. TALEN Expression Vectors

    Type
    Collection
    ... can be purchased together with the Joung Lab Individual REAL TALE Repeat Plasmids in the Joung Lab REAL.... 2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70 NI RVD A 32287 JDS71 HD RVD C 32288 ...

  15. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...particular gRNA. In the enChIP (engineered DNA-binding molecule-mediated ChIP) system, catalytically inactive...avoiding known transcription factor and other protein binding sites. Browse, sort, or search the tables below...

  17. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...

  18. All Antibodies

    Type
    Collection
    ... our trusted partner labs. On this page you will find all the antibodies currently available from our ... the table below or visit the following pages to find antibodies in our curated collections: Tags and ...

  19. Optogenetics Plasmid Collection

    Type
    Collection
    ...Find optogenetics plasmids such as microbial opsin and optical switch tools to measure and manipulate...Interested in more news about optogenetics research? Find related posts on Addgene's blog. Microbial Opsin...

  20. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions....single plasmid, though many still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3...
Showing: 81 - 100 of 135 results