...to learn about the applications of fluorescent proteins and to find the plasmids that are available from...Monomer not a Monomer? Addgene's plasmid repository contains a variety of fluorescent protein plasmids. Use...more about the many applications of fluorescent proteins (FPs) and to find the plasmids that are available.... Regulate Biological Activity Use fluorescent proteins to modulate biological activity, like transcription...consisting of ORFs tagged with a variety of fluorescent proteins, as well as over 100 empty backbones. Fluorescent...Jin Zhang Lab at UCSD Properties of Fluorescent Proteins from Kurt Thorn and UCSF Molecular Expressions...

...Neuroscience : Antibodies targeting proteins for neuroscience research. Institute for Protein Innovation Collection...other types of affinity reagents such as scFvs, DARPins, Fabs, and secondary antibodies. To learn more ...Collection : Antibodies to highly conserved proteins and other difficult-to-target antigens that are intractable... represent the official views of the National Institutes of Health. Need Some Help? Browse some of our... Antibody Guide Antibody Protocols Recipient Instructions Antibody Blog Posts Addgene Help Center...

... libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors, and more....Addgene Plasmid Collections Addgene's repository contains 156,588 plasmids . We've gathered our curated ...Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral Plasmids...Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic... Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors...RESOLUTE Plasmid Collection for Solute Carrier Proteins (SLCs) Structural Genomics Consortium Zinc Finger...

...cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains a potential cleavage...credit: Maya Kostman for the Innovative Genomics Institute Addgene COVID-19 Resources The following types...distribute SARS-CoV-2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression Spike...Return to top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified as...SHERLOCK and DETECTR . Feng Zhang's lab at the Broad Institute has utilized the natural RNAse activity of the...UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad Institute has published information and protocols for utilizing...

...its genome and several structural and enzymatic proteins, including reverse transcriptase and integrase...genome, which can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide for...Plasmid Type Description PI 8449 pUMVC Packaging Contains MoMLV gag and pol; use with envelope plasmid Weinberg... Weinberg 12371 pCL-Eco Packaging Contains MoMLV gag, pol, and env Verma 35614 pBS-CMV-gagpol Packaging...database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo...modified version of pRX-tight Puro encoding NAT instead of PAC Iglehart 20672 MSCV-IRES-GFP MSCV For transgene...

... distributed by Addgene were developed by the Institute for Protein Innovation (IPI). For these antibodies...more about these antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage...with the plasmid(s) encoding the heavy and light chains. Antibodies are expressed for 7 days and then purified...opens in a new window) . Western Blotting (WB) Proteins are extracted from cells, separated by SDS-PAGE...thawing on ice, and then testing for loss of potency against a non-treated control in one of its recommended... Antibody Guide Antibody Protocols Recipient Instructions Antibody Blog Posts Addgene Help Center...

...recombinases 10–14 . Figure 2: INTRSECT works by inserting short, intronic sequences into the open reading...recognition sequences (e.g. Lox sites, FRT sites) inside of the introns (A,D). The addition of these recombinase...intersectional experimental design. The Deisseroth Lab maintains a Standard Operating Procedure (Link opens in ...additional fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies... NOT Cre Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations 55645... NOT Cre Dual recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations 137148...Deisseroth K, Zhao F, Luo MH, Gong L, He M, Zhou P, Paninski L, Li B. 2017. The central amygdala controls learning...

...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial fields,...and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression - Pertz...MultiSite Gateway Kit : Creating three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous...Vaccinia virus ORF library : Expression library that contains each of the predicted open reading frames for ... : 285 vectors for expression, markers, fusion proteins, etc - Lindquist Lab Synthetic Biology BIOFAB ...Restoration of prototrophy in common S. cerevisiae lab strains - Ralser Lab Return to top Resources for...

...depending on the insert type and envelope used. Please consult with your institution’s Biosafety Committee...that they no longer encode for more virus, but instead encode for a specific gene or other research interest...with specific genomes (Fig. 1A) . In other words, instead of a virus infecting a host and giving rise to ... same way that naturally occurring viruses do. Instead, the genes that are required for viral replication... be produced. Each of these plasmids, however, retains the properties of any typical plasmid and thus ...

...Investigators (PIs) PI Institution NETRF Project Title Hans Clevers Hubrecht Institute Modeling Neuroendocrine...Organoids Matthew Meyerson Dana-Farber Cancer Institute Finding the Causes of Small Intestinal Neuroendocrine...Neuroendocrine Tumors Ramesh Shivdasani Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine ... and Carcinoid Tumors Bradley Bernstein Broad Institute Identifying Altered Epigenetic States and Drivers...

...therapeutics, and vaccines against COVID-19 all rely on DNA encoding viral proteins as a first step for many...Browse plasmids encoding SARS-CoV-2 viral proteins. Depositor...enable researchers to express SARS-CoV-2 viral proteins in several expression systems. Addgene has partnered...2 and common coronvirus plasmids based on: Gene insert (e.g. spike, envelope, nsp3, OC43, NL63, HKU1, ...

... Plasmid Collections Fluorescent Proteins In Vivo Imaging Guide Fluorescent Proteins: In ...fluorescent proteins. Plasmid...protein interactions in living cells and live mice. Proteins for in vivo imaging have emission near or above... background signals. Near-infrared fluorescent proteins for in vivo imaging are derived from bacterial...

...boundary defined by anti-CRISPR proteins or running into other proteins bound to DNA, and the break can...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication...Publication Plant ID Plasmid Gene/Insert Promoter PI Publication Last reviewed on: January 30, 2025 Do you ...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable...Marker PI Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you...

...They encourage instructors teaching different types of courses, at any type of institution, and at different...facilitates scientific collaborations between institutions. With these plasmids, faculty can begin a sustainable...subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs Community...External Collaboration Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan KP...

...School Ali Cetin Allen Institute for Brain Science Ben Deverman Broad Institute Katherine Matho Cold Spring...Ali Cetin and Shenqin Yao, Allen Institute Ben Deverman, Broad Institute Katherine Matho, Cold Spring Harbor...meeting. The goal of this year’s workshop is to brainstorm practical strategies for using combinations of...

...Additional steps, including request approval, your institution’s approval of the MTA, and shipping, mean that... typically takes 1–4 days, depending on your institution. AAV Production 6–8 weeks Quality is our priority...vector experience, from methods to troubleshooting insights, you make research more reproducible for scientists...information on its performance. ID Plasmid Gene/Insert Promoter PI Additional Resources Viral Vector Production... that complement published data, capturing the insights that often go unshared. Help Center Check out ...

...Backbones Fluorescent Proteins Other Resources Background Addgene’s repository contains many plasmid tools...Fluorescent Proteins The table below highlights plasmids that feature fluorescent or luminescent proteins for ...engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids containing...

...and pICPIS-CB. pICPIS-EF1 contains the EF1 promoter while pICPIS-CB contains the Chicken-beta actin promoter...plates — if your insert is successfully cloned into this vector, transformants will instead form white colonies...promoter pLL3.7-TNFa-shRNA3 72598 Expresses shRNA against mouse TNF-alpha mRNA from the mouse U6 promoter...

...Plasmid Collections Fluorescent Proteins Activity Regulation Fluorescent Proteins: Activity Regulation More ...Biosensors Optogenetics Background Fluorescent proteins such as GFP have long been used for labeling, ... methods are emerging to use these fluorescent proteins to modulate biological activity. Early tools allow...