...generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP...coinjected to generate mutations via imperfectly repaired DSBs. Coinjection of phsp70-Cas9 and plasmids ... of an ssODN donor template can mediate gene replacement by homologous recombination. For the sgRNA, vector...for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed....

...developed three platforms for engineering TAL effector repeat arrays of any desired length: the REAL (Restriction... three of these platforms assemble TAL effector repeat arrays on a single architectural framework and ...methods are identical in sequence. TAL effector repeat arrays produced on this framework has yielded TALENs...Lab REAL Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast...for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with the REAL...

...limited use label licenses (LULLs). Titer For AAV preps produced through our Packaged on Request service... we may expect lower titers for a particular AAV prep, mostly likely due to one of the following reasons...moving forward with a helpful solution. You must reply to that email within 10 business days in order to...been completed at the time of shipment. If any discrepancies are identified during this analysis, you will...moving forward with a helpful solution. You must reply to this email within 10 business days in order to...

...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...starter cell via the EnvA receptor. RABV is able to replicate in the starter cell using the G-protein, which...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...International Resource Center (ZIRC) - A US-based repository for zebrafish strains and materials and information...European Zebrafish Resource Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT...

...leucine-rich repeat-containing G protein-coupled receptor 4 GPR48 LGR5 leucine-rich repeat-containing G... to the formation of a high avidity BCR through repeat cycles of expansion. Chemokines Chemokines, or ... to the formation of a high avidity BCR through repeat cycles of expansion. Symbol Name Synonyms AKT1 ...MGC102867 SEMA5A sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain ... semF SEMA5B sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain ...LGR1, MGC141667, MGC141668, ODG1 GAL galanin prepropeptide GALN, GLNN, GMAP, MGC40167 GALP galanin-like...GHRFR, GHRHRpsv, GRFR GHRL ghrelin/obestatin prepropeptide MTLRP, obestatin GHSR growth hormone secretagogue...

...be used with a repair template to introduce specific edits via homology-directed repair (HDR). Want more...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...

... modification, including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids...regulatory elements, including promoters, terminators, repressors, activators, and more. Also contains pre-assembled...BioBrick Box Mukhopadhyay Lab Efflux Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors...Foundation Registry of Standard Biological Parts - Repository of community-created BioBricks BioBricks Foundation...

...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime editing is a “search and replace” gene...Find prime editor plasmids for (search and replace) genome editing. CRISPR...heteroduplex is resolved by the cell’s mismatch repair (MMR) system. Alternatively, if the edited 3’ flap...

...Melina Fan/Leila Haery 5:00 PM Adjourn Meeting Preparation For Speakers: Each speaker will give a 20 minute... reagents)? For All Attendees: To help us best prepare and compile resources for this meeting's publication...meeting will be an openly shared communication representing a reference for AAV use in neuronal intersectional...commitment to open science! Please see the meeting preparation section for how to best help contribute to this...

...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial...distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause....Salmonella sp. Shigella sp. Staphylococcus sp. Streptococcus sp. Synechococcus sp. Thermus sp. Vibrio sp....plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...

...Biosafety Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler...transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452...

...populations. For more information, see the bioRxiv preprint describing ICE . Deep Sequencing Data Analysis...non-homologous end joining (NHEJ) and homologous directed repair (HDR) frequency. CRISPResso requires two inputs...for genome editing, as well as gene regulation (repression and activation). Genome support for bacteria ... lab: Tips for CRISPR design, homology directed repair and cloning . CRISPR Discussion Group (moderated...

... engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead of ...isotype. Overview of application Fix your samples. Prepare your samples using permeabilization, antigen retrieval...and controls follow that of IF (above). Sample preparation, signaling excitation, and assay readout vary...must also be run. Unique samples (non-biological replicates) often each require their own set of controls...conjugating them in the lab? How will the sample be prepped, and will this or other factors affect epitope ...

...bound to DNA, and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3...

...and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination of metabolomics...deorphanizing engine. Genetic and proteomic interactions represent the framework that provides biological context...the Resolute Partnership using their RESOLUTE repository feedback form . If you have further questions...

...Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of different...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

...Endogenous Zebrafish Gene Targets Recent published reports have shown that engineered zinc finger nucleases...greater in a well-characterized bacterial two-hybrid reporter system but have NOT yet been tested for activity...OZ552 OPEN OPEN gGTCATCTGCcattcGAGGCGGACa REST corepressor 2 OZ553 and OZ554 OPEN OPEN gCTCACCATCtgttggaTGCGATGGAa...

...elegans genome is well studied and hundreds of reporters have been developed and characterized which allow...plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...Contains a set of plasmids for building homologous repair templates that incorporate a self-excising drug...

...164499 EZH2-mEGFP AICSDP-83 mEGFP EZH2 Polycomb repressive complex 2 164500 POLR2A-mEGFP AICSDP-117 mEGFP...II 168798 TERF2-mEGFP AICSDP-77 mEGFP Telomeric repeat-binding factor 2 (TRF2) Telomeres 168799 CTCF-mEGFP...Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the ...