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Addgene
Showing: 61 - 80 of 115 results
  1. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...in order to proceed with any adjustments to the original order. Turnaround Time Production of a Packaged...the approval of the MTA by your institution. Our final step of the QC process, viral genome sequence verification...in order to proceed with any adjustments to the original order. Experimental Disclaimer Due to the novel...
  2. EXtracellular Plasmid RESource (EXPRESs) Consortium

    Type
    Collection
    ...surface receptor and secreted proteins as soluble recombinant molecules....surface receptor and secreted proteins as soluble recombinant molecules. The proteins are typically used in...series of expression vectors which have different C-terminal tags. Most vectors are based on the pTT3 plasmid...
  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...gamma GFP Tom Misteli 55068 mCherry-LaminA-C-18 Nuclear Envelope LaminA1 mCherry* Michael Davidson 98831 ...Davidson 98822 Lck-mTurquoise2 Plasma Membrane Lck N-terminal lipidation signal mTurquoise2 Dorus Gadella 98821...98821 Lck-mScarlet-I Plasma Membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella 50057 pLYS1...
  4. Distribution to Industry

    Type
    Collection
    ...Labeling from New England Biolabs Cancer Research from Sinai Health System Plasmids from the Structural Genomics... Antibodies Addgene distributes ready-to-use recombinant antibodies made from plasmids in our collection...as by our trusted partner labs. Learn More ID Recombinant Antibody Reactivity Recommended Applications ...
  5. Qi Lab CRISPR Page

    Type
    Collection
    ...Description 44246 pdCas9-humanized A catalytically inactive, human codon-optimized Cas9 expression plasmid...plasmid 44247 pdCas9::BFP-humanized A catalytically inactive, human codon-optimized Cas9-BFP fusion expression...constructs 44249 pdCas9-bacteria A catalytically inactive bacterial Cas9 expression plasmid 44250 pwtCas9...
  6. AAV Packaged on Request

    Type
    Collection
    ...as they receive their AAV prep from Addgene — eliminating the steps to produce, purify, and titer viral...research-grade use. Many chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository...vivo testing. We recommend that you consult the original paper of your chosen AAV plasmid for more information...
  7. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...virus, plasmids expressing both the Cre and Flp recombinases to remove genes flanked by either loxP or frt...gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express gene of interest...variety of reporters as well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors...
  8. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...Aqua Cyan Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian...pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene of...pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian Express a gene ...
  9. Viral Vectors

    Type
    Collection
    ...Stable RNA <8 Kb 80-130 Dividing Yes Moderate * Recombinant AAV has a low frequency of target cell genome... Lab Lentivector Toolbox Guide to Generating Recombinant Adenoviruses Eric Campeau's Viral Vector Guide...Toth K. Adenovirus vectors for gene therapy, vaccination and cancer gene therapy. Curr Gene Ther. 2013...
  10. Open Enzyme Collection

    Type
    Collection
    ...9N7polA (CT) 9 degrees N-7 DNA Polymerase chain terminating 165511 pOpen-K12polLF DNA Polymerase I, Large...methyltransferase 165520 pOpen-T4 PNK T4 PNK polynucleotide kinase 165521 pOpen-lambda red operon lambda red operon...pOpen-BovDNTT Bovine DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional Resources...
  11. COVID-19 Resources

    Type
    Collection
    ... found on this page: SARS-CoV-2 Antibodies : Recombinant monoclonal antibodies that target SARS-CoV-2 ... Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse monoclonal antibody targeting the SARS-... Nucleocapsid Protein [mBG86] - Clone mBG86, recombinant mouse monoclonal antibody targeting the SARS-...
  12. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...guanosine. RNA editors : Cas13 fused to the ADAR2 deaminase convert adenosine to inosine on RNA. Inosine is..., Davidson AR. 2013. Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system. Nature...Cell . 154(2):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide...
  13. Antibodies

    Type
    Collection
    ...Overview of Addgene's ready-to-use monoclonal recombinant antibody collection. These affinity reagents ... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate scientific research...
  14. TALEN Expression Vectors

    Type
    Collection
    ...assembly are different among the three methods, the final products are the same). All expression vectors listed...Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70 NI RVD A 32287...
  15. EXPRESs plasmids for human erythrocytes

    Type
    Collection
    ... collection plasmids for expressing secreted recombinant proteins from human erythrocytes that was created...bio-CD4d3+4-APMAP 73092 bio-CD4d3+4-TMEM24 73093 GlycophorinA-CD4d3+4-bio 73094 GlycophorinB-CD4d3+4-bio 73095...
  16. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Catalytically inactive CRISPR plasmids for purification of target loci.... molecule-mediated ChIP) system, catalytically inactive dCas9 is used to purify genomic DNA bound by the...
  17. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Expression vector for secreted proteins with N-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac...Expression vector for secreted proteins with C-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac...
  18. FlyCRISPR

    Type
    Collection
    ... can mediate gene replacement by homologous recombination. For the sgRNA, vector pMB60 allows in vitro...51026 U6-BbsI-crRNA : Generates crRNA for use in combination with tracrRNA. 51434 pHD-DsRed : vector for generating...
  19. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...a ntiviral de fense) complex, comprised of a combination of Cas5, Cas6, Cas7, Cas8, and Cas11. Cascade...non-specifically along the way. Cas3 initiates a final double-strand break, either due to a boundary defined...
  20. Resolute Plasmid Collection

    Type
    Collection
    ...partnership of 13 members from industry and academia financed by the Innovative Medicine Initiative (IMI) of...Figure 1: Representation of RESOLUTE mission. The combination of metabolomics and genome engineering forms ...
Showing: 61 - 80 of 115 results