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Showing: 61 - 80 of 85 results

  1. Rett Syndrome

    Type
    Collection
    ...typically arise spontaneously, although in rare cases Rett syndrome can be inherited. In females, random...R306C) account for almost 46% of all Rett syndrome cases. Research Milestones 1992 - MECP2 is discovered ...Knock-in of NLuc-tdTomato at endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional...hand use in girls: Rett’s syndrome: report of 35 cases. Ann Neurol . 14, 471–479. (Link opens in a new ...

  2. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...markers. The FP together with the protothropic cassette can be amplified with the following primers: FW...Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Plasmids Bas Teusink Lab Materials...in a new window) Sheff MA, Thorn KS. Optimized cassettes for fluorescent protein tagging in Saccharomyces...Bydder SF, Zhou Y, Nielsen LK. Dual gene expression cassette vectors with antibiotic selection markers for ...

  3. Lentivirus Plasmids

    Type
    Collection
    ...shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs 11619...shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler 1730... plasmid with a chimeric 5’LTR, Any expression cassette (promoter and gene of interest) can be cloned ...interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). See...

  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...mE10 CARD11 caspase recruitment domain family, member 11 BIMP3, CARMA1, MGC133069 CBL Cas-Br-M (murine...retroviral transforming sequence C-CBL, CBL2, RNF55 CBLB Cas-Br-M (murine) ecotropic retroviral transforming sequence...DKFZp779F1443, FLJ36865, FLJ41152, Nbla00127, RNF56 CBLC Cas-Br-M (murine) ecotropic retroviral transforming sequence...BRAF1, FLJ95109, MGC126806, MGC138284, RAFB1 CASP3 caspase 3, apoptosis-related cysteine peptidase CPP32...ASBT, ISBT, NTCP2 TAP1 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) ABC17, ABCB2, APT1, D6S114E...TAP1*0102N, TAP1N TAP2 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) ABC18, ABCB3, APT2, D6S217E...IMD1, MGC126261, MGC126262, PSCTK1, XLA CARD11 caspase recruitment domain family, member 11 BIMP3, CARMA1...

  5. Adenovirus Plasmids

    Type
    Collection
    ...deletion in E3 and insertion of GFP expression cassette. Bunz 179202 pAd5-B6/7 ∆E3-GFP Adenoviral A block...deletion in E1 and insertion of GFP expression cassette. Bunz 16402 pShuttle Shuttle For production of...Shuttle Modified pShuttle-CMV plasmid with Gateway cassette; See article for additional plasmids Zhang 50957...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS...Patrick Caswell 179900 pJET-59-DExCon-mTagBFP2-R25 RAB25 mTagBFP2 TRE3GS Parkinson's Patrick Caswell 180016...sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-Cas9 HTT U6, 7sk...MS2 CMV Parkinson's Niels Gehring 66818 pCCL-PGK-SPdCas9-BFP-DNMT1 DNMT1 PGK Hereditary sensory neuropathy...Episodic ataxia Xiaolong Wang 159912 pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3 HTT CMV Huntington's Larry Zweifel 160435...Wernig 176852 UBQLN2_450-624W UBQLN2 T7 ALS Carlos Castañeda 176915 pcDNA3.1(+)_Paragranulin+linker 1 GRN...Parkinson's Frank Soldner 180437 Px330-EGFP-LRRK2-CRISPR/Cas9 LRRK2 U6 Parkinson's Frank Soldner 180439 STR-KDEL_PGRN-SBP-mCherry...

  7. Antibody Guide

    Type
    Collection
    ...expression. Monoclonal antibodies are necessary in cases where extreme specificity is important. Their epitope...when epitope specificity is not a concern or in cases where you may want to identify or collect the protein...azide, as it acts as an inhibitor to HRP. In those cases, thimerosal can be used as an antimicrobial agent... using the above process and try again. In some cases, it may be preferable to buy small aliquots of many...

  8. Plasmids for Stem Cell Research

    Type
    Collection
    ...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes. piggyBac transposition reprograms fibroblasts...CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice...

  9. Brain Initiative Collection

    Type
    Collection
    ...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV8 pAAV-Ef1a-DIO-PPO-Venus...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV9 pAAV-Ef1a-DIO-PPO-Venus...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 140135-AAV1 pAAV-EF1a-iCreV can...

  10. AAV Molecular Tools

    Type
    Collection
    ...cell ablation. 2 Jessell , Azim 45580 pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, ..., bicistronic expression of designer pro-taCasp3 and TEVp for studying cell ablation. 5 Shah , Wells Don...

  11. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...titered by droplet digital PCR (ddPCR) . In some cases, we may expect lower titers for a particular AAV...titer due to toxicity or other reasons For these cases, Addgene will promptly notify you by email within...

  12. Mammalian RNAi Tools

    Type
    Collection
    ...under the mouse U6 promoter; A CMV-EGFP reporter cassette is included in the vector to monitor expression...shRNA under the H1 promoter; shRNA or a H1-shRNA cassette from another vector (e.g. pSUPER) can be cloned...

  13. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...Site (5' to 3'; half-sites in CAPS, spacer in lowercase) aak1 TAL3208 & TAL3209 TCTTTGACTCCCGGAGAGagctggtgggctccgggCCAGGTTCTGGAGGGGGA...TGGACGCGGAGCTGTTCTcggagcaggagctgacCTGCTCCATCTGTCTGGA casp8ap2 TAL3038 & TAL3039 TATAACAAATCCCACCACattgcccgatatcttgAATACTTCCAAAACAAAA...

  14. Depositor Collections

    Type
    Collection
    ...Locus-specific ChIP technologies (iChIP & enChIP) using dCas9 - H. Fujii Disease Research Michael J. Fox Foundation...

  17. TALENs for Endogenous Human Genes

    Type
    Collection
    ...Site (5' to 3'; half-sites in CAPS, spacer in lowercase) ABL1 TAL2380 & TAL2381 TACCTATTATTACTTTATggggcagcagcctggaaAAGTACTTGGGGACCAA...

  18. Distribution to Industry

    Type
    Collection
    ...Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of human TP53...

  19. Arf GTPase Family

    Type
    Collection
    ...GAPs: concepts and challenges Sztul E, Chen PW, Casanova JE, Cherfils J, Dacks JB, Lambright DG, Lee FS...

  20. Viral Production

    Type
    Collection
    ... contamination. To date, Addgene has never had a case of mycoplasma contamination. In the event of contamination...
Showing: 61 - 80 of 85 results