...methylated DNA and the recruitment of the NCoR complex for proper nervous system function. While there...RSRT cell line collection (186.8 KB) . To order, please email [email protected] The following cell lines are...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page...precise editing of mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction...expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources can be found below...cell line repository (Link opens in a new window) Sampled - one of the world's largest biorepositories (Link...your plasmids! To get started with your deposit, please click here ....

...total protein content of the sample is determined. Only antibody samples with >90% purity are distributed... webpage. To learn more about these antibodies, please visit the Institute for Protein Innovation Antibody...using a NanoDrop spectrophotometer. If needed, the sample is adjusted by diluting or concentrating to a final...Coomassie staining. For Coomassie staining, the sample is separated via a denaturing SDS-PAGE gel alongside...specific QC experiments were performed on your lot, please email [email protected] . Immunocytochemistry (ICC...

...imaging techniques, including that it is easy to implement due to the simplicity of gRNA design, programmable...for different genomic loci, capable of detecting multiple genomic loci, and compatible with live cell imaging...copies of labeled proteins to the given region. For example, chromosome-specific repetitive loci can be efficiently...visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity. A non-repetitive...genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome painting...

...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Wickersham ...pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant rabies virus AAV1 Wickersham ...lacks the ability to infect neighboring cells. For example, G-deleted rabies lacks the viral glycoprotein...infect any neighboring cells. Thus, through complementation, G-deleted rabies virus can spread to monosynaptically...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

... seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants...biology - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR resources include: A Multipurpose...CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants - Yao-Guang...

...far more complex than using CRISPR to modify a single genomic locus. Before ordering, please review the...consist of thousands of plasmids, each containing multiple gRNAs for each target gene. In a CRISPR screening...Root 2nd 2 40,710 Inzolia Human CRISPR/Cas12a Multiplex Knockout 209551 209552 Knockout Human Doench 3rd...117725 Knockout Human Vakoc 3rd 6 3,051 Human Lipid Droplet and Metabolism Library 191535 Knockout Human Olzmann...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...

... browse the gene list below. For proteins with multiple subunits or isoforms, individual links to each...available plasmids for the gene. For components with multiple subunits or isoforms, individual links to each...EXOC2 EXOC3 EXOC4 EXOC5 EXOC6 EXOC7 EXOC8 Exocyst complex component FGFR FGFR1 FGFR2 FGFR3 FGFR4 Fibroblast... cis/trans isomerase, NIMA-interacting 1 PLXNB1 Plexin B1 PPP1CA Protein phosphatase 1 catalytic subunit...B1 RPTOR Regulatory associated protein of MTOR, complex 1 SAV1 Salvador family WW domain containing protein...

...movement. More than ten million people worldwide are living with PD, mostly people over the age of 60. There...caused by abnormal expansion of a repeating CAG triplet series within the huntingtin (HTT) gene. An individual...variation and lifestyle or environmental factors. For example, variations of Apolipoprotein E (APOE), such as...collection of neurological mouse models including complex and monogenic diseases. Alzforum (Link opens in...

...PGK Retroviral Lowe 33344 pLEW100cre-EP1-6G Cre T. brucei Cross 33345 pLEW100cre-EP1 Cre Trypanosoma brucei...blog post . Based on these Cre-lox recombination principles, scientists have developed constructs to activate...pSkipFlox split Cre Pfcrt P. falxiparum Spielmann 86641 pLenti-hSynapsin-Cre-WPRE Cre hSyn Lentiviral Wang 86794...donor/transfer vector Cbh AAV Eisenhoffer 131786 pLenti-CreLite CreLite system components, PhyBdeltaCreC...in the indelible marking of mouse neurons with multiple distinct colors Mammalian Sanes See article Multibow... to facilitate Cas9 mediated genome editing in multiple tissues. FLP/FRT: Alternative recombinase system...

...cut S. pyogenes 26028531 Huangfu OCT4 H. sapiens multiple, see article 69537 activate S. pyogenes 26352799... pyogenes 25336740 Christiaen EGFP A. victoria multiple, see article 60071 dCas9-FokI S. pyogenes 24770325...23817069 Calarco Kras, p53, and Lkb1 M. musculus multiple, see article 60224 cut S. pyogenes 25263330 Zhang.... pyogenes 23929340 Kamoun PDS1 N. benthamiana multiple, see article 49772 cut S. pyogenes 24112467 Kamoun...AGCACTGTTGCCGGCCGAGG 79006 cut S. pyogenes 26944679 Shaw Please refer to the primary article to confirm details...add validated gRNA sequences to our datatable, please download the following spreadsheet, fill out as...