Skip to main content
Addgene
Showing: 41 - 60 of 68 results

  1. FlyCRISPR

    Type
    Collection
    ...Browse CRISPR Cas9 gRNA plasmids for drosophila research from the O'Connor-Giles, Wildonger, and Harrison...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Harrison Lab - ...template: example : Resources: Protocol for generating gRNAs 106.5 KB flyCRISPR website contains additional information...information CRISPR Optimal Target Finder Identifies gRNA target sequences from an input sequence and checks...replacement by homologous recombination. For the sgRNA, vector pMB60 allows in vitro production of RNA ...

  2. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi Libraries ...Reduced Double-gRNA Library 137999 Knockout Human Parts 3rd 3 59,576 Human Whole Genome sgRNA iBAR Library...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is a useful...experiments, due to the relative ease of designing gRNAs and the ability to modify virtually any genetic ...thousands of plasmids, each containing multiple gRNAs for each target gene. In a CRISPR screening...libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore, you must be equipped...

  3. Lentiviral Prep Service

    Type
    Collection
    ...RNA guide (gRNA) that harbors targeting sequences. Browse our repository for a list of gRNA plasmids targeting...of Cas9 + ACE2 gRNA Sheltzer 153015 TMPRSS2 g1 + Cas9 Expression of Cas9 + TMPRSS2 gRNA Sheltzer Viral... Doench , Root 73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2...pooled libraries 73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro...Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation...containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 ...pooled libraries 73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro...

  4. Plant Plasmids and Resources

    Type
    Collection
    ...assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice... different functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR...Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots...

  5. Qi Lab CRISPR Page

    Type
    Collection
    ... fusion expression plasmid 44248 pgRNA-humanized A customizable gRNA expression plasmid for use with either...use editing bacterial genomes 44251 pgRNA-bacteria A customizable gRNA expression plasmid for use with the...CRISPR CRISPRi Cas9 gRNA plasmids from the Qi lab CRISPR...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts We have recently ...resistance. The second plasmid ( pgRNA_bacteria ) contains a constitutive sgRNA expression cassette on a ColE1...The second plasmid ( pgRNA_humanized ) contains a murine U6 promoter controlled sgRNA cassette, wherein ...dCas9) protein and a customized s ingle g uide RNA (sgRNA) designed with a 20-basepair complementary region...

  6. Huang Lab CRISPR Plasmids

    Type
    Collection
    ...CRISPR Cas9 gRNA plasmids from Dr. Xingxu Huang's lab....You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts A human codon-optimized...

  7. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...with paired gRNAs to lower off-target cleavage frequency. Truncated gRNAs: Truncated gRNAs display less...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution... 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA complex produces a DSB at the target genomic locus...is not only simple, but also scalable. Multiple gRNAs targeting multiple loci can be easily expressed ...it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are...marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol . . PMID: 27088723...

  8. Calarco Lab CRISPR Plasmids

    Type
    Collection
    ...CRISPR Cas9 gRNA plasmids from Dr. John Calarco's lab....You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts A CRISPR/Cas system...PU6::unc-119_sgRNA : unc-119targeting sgRNA. 46170 PU6::klp-12_sgRNA : klp-12targeting sgRNA. Please note.... elegans . The protocol for customizing the sgRNA expression plasmid can be located in the Materials...

  9. Doudna Lab CRISPR Plasmids

    Type
    Collection
    ...CRISPR Cas9 gRNA plasmids from Dr. Jennifer Doudna's lab....You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Assorted Cas9 nucleases...

  10. Kamoun Lab CRISPR Plasmids

    Type
    Collection
    ...CRISPR Cas9 gRNA plasmids from Dr. Sophien Kamoun's lab....You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts A CRISPR/Cas system... an sgRNA uder the Arabidopsis U6 promoter. 46966 pICH86966::AtU6p::sgRNA_PDS : Expresses an sgRNA targeting...module. 49772 pAGM4723::AtU6p::sgRNA2-2x35S-5'UTR::Cas9::NOST-AtU6p::sgRNA1 : Level 2 construct....manuscript for the protocol on assembling customised sgRNA expressing plasmids. Questions regarding the protocol...

  11. Boxem Lab CRISPR Plasmids

    Type
    Collection
    ...CRISPR Cas9 gRNA plasmids from Dr. Mike Boxem's lab....You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts We adapted the S....vectors for the delivery of the synthetic guide RNA (sgRNA). Each of the Cas9 vectors contains the Cas9 coding...with and without a C-terminal EGFP fusion. For the sgRNA, vector pMB60 allows in vitro production of RNA ...

  12. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...consider that wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to have off-target... is that you can express two different chimeric gRNAs with the Cas9 nickase which will together introduce...with efficiency similar to using a single chimeric gRNA. At the same time, the off-target effects are reduced...sequence, and the concentration of Cas9 and guide RNA (gRNA). These considerations only matter if the PAM sequence...which of your colonies carry the correct insert (gRNA) in pX330 / pX335 with BbsI/AgeI double digestion... plate, co-deliver into the cells with the Cas9/sgRNAs vector (like PX330). For large changes (>100bp ... a typical size (~ 5kb) donor plasmid with Cas9/sgRNAs vector, for one well in 24-well plate. A good example...

  13. Jaenisch Lab CRISPR Plasmids

    Type
    Collection
    ...CRISPR Cas9 gRNA plasmids from Dr. Rudolf Jaenisch's lab....You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-on is an RNA-guided...guide RNA (sgRNA). While the dCas9 protein provides the transactivation activity, the sgRNA component ...activators and sgRNAs can be separately transfected (pmax dCas9 activators [48223~48227] + sgRNA expression...expression vector or the linear DNA containing the sgRNA expressing fragment produced by PCR), or expressed from... via the links below: Table 1. dCas9 Activators sgRNA Dual Expression ID Plasmid 48236 pAC2-dual-dCas9VP48...expression construct expressing both dCas9VP48 and sgRNA from separate promoters. 48238 pAC152-dual-dCas9VP64...

  15. Lentivirus Plasmids

    Type
    Collection
    ...plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search "lenti...

  16. Plasmid Collections

    Type
    Collection
    ...libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids ...

  17. Bacterial Expression Systems

    Type
    Collection
    ...see here . pgRNA-bacteria 44251 CRISPR Stanley Qi Expression of customizable guide RNA (gRNA) for bacterial...can be combined with expression of a gRNA from a plasmid like pgRNA-bacteria to knock down endogenous gene...Marraffini Bacterial expression of Cas9 nuclease gRNA. For a more comprehensive list of our bacterial ...

  18. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...75637 EIF2AK2 gRNA (BRDN0001146342) EIF2AK2 hU6 VWM disease David Root 75638 EIF2AK2 gRNA (BRDN0001148577...75639 EIF2AK2 gRNA (BRDN0001148668) EIF2AK2 hU6 VWM disease David Root 76195 ERBB4 gRNA (BRDN0001162372...David Root 76196 ERBB4 gRNA (BRDN0001149467) ERBB4 hU6 ALS David Root 76197 ERBB4 gRNA (BRDN0001146197) ERBB4...David Root 76198 ERBB4 gRNA (BRDN0001162324) ERBB4 hU6 ALS David Root 76809 PANK2 gRNA (BRDN0001149459) PANK2...76810 PANK2 gRNA (BRDN0001162474) PANK2 hU6 Hallervorden-Spatz disease David Root 76811 PANK2 gRNA (BRDN0001162362... PANK2 gRNA (BRDN0001144887) PANK2 hU6 Hallervorden-Spatz disease David Root 76838 PRKAR1B gRNA (BRDN0001146606...PRKAR1B gRNA (BRDN0001144765) PRKAR1B hU6 Dementia and/or parkinsonism David Root 76840 PRKAR1B gRNA (BRDN0001147089...

  19. Microbiology Resources

    Type
    Collection
    ...CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast CRISPR resources ...

  20. Worm Expression Resources

    Type
    Collection
    ...resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging...
Showing: 41 - 60 of 68 results