...and high-titer viral preparations of plasmids that are currently in our repository. ID Plasmid Description...sites AAV Uchida Cre Reporters and Tools In this subtype of loxP plasmids, reporter genes indicate which...Mammalian Pelczar 62732 Cre Reporter DsRED and EGFP Cre recombinase reporter Lentiviral Geijsen 65726 pLV-CMV-LoxP-DsRed-LoxP-eGFP...Vectors Cre-containing Plasmids loxP Constructs Cre Reporters & Tools Additional Resources References Background...Fluorescent Cre: The fusion of Cre to a fluorescent reporter enables visualization of Cre expression. Optimized... of choice. We also offer ready-to-use viral preparations of select Cre expression plasmids. Addgene's...HRE:GFP-pGK:Cre Cre and hypoxia-responsive GFP reporter HRE Lentiviral Jacks 69570 pAAV-EF1a-N-CretrcintG...

...Plasmid Description PI Viral Preps Addgene distributes ready-to-use viral preps made from plasmids in our... BRAIN Initiative collection of plasmids, viral preps, and antibodies here. Collections...the research community. Browse plasmids , viral preps , and antibodies created with support from the BRAIN...use. Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden 108912...Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123308...Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123309...

...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for... Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...available in standard format and as a Lentiviral prep . ID Plasmid Description Mutations Industry PI Due...pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly luciferase...destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...

.... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...be used with a repair template to introduce specific edits via homology-directed repair (HDR). ID Plasmid...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...

...amino acid repeats. These repeats only differ from each other by two amino acids, their repeat-variable ... of 18 TAL effector repeats. The kit can be used to make arrays from 12-31 repeats. Perhaps one of the... to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (ZF) nuclease. Zinc fingers...if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit... would expect that assembling relatively small, repeat-laden DNA regions into a single construct would...from Addgene , custom arrays consisting of 12-31 repeats can be assembled and inserted into a variety of...

...- and serine-containing peptide linker (GGSGGS) repeat pET28CLY2 Peptide linker standard consisting of...of ECFP and EYFP connected by 2 GGSGGS repeats pET28CLY3 Peptide linker standard consisting of ECFP and...and EYFP connected by 3 GGSGGS repeats pET28CLY4 Peptide linker standard consisting of ECFP and EYFP connected... connected by 4 GGSGGS repeats pET28CLY5 Peptide linker standard consisting of ECFP and EYFP connected...connected by 5 GGSGGS repeats pET28CLY6 Peptide linker standard consisting of ECFP and EYFP connected by 6 GGSGGS...GGSGGS repeats pET28CLY7 Peptide linker standard consisting of ECFP and EYFP connected by 7 GGSGGS repeats...consisting of ECFP and EYFP connected by 8 GGSGGS repeats pET28CLY9 Peptide linker standard consisting of...

...tyrS gene knocked out, tyrTV gene replaced by tolC, and tyrU gene replaced by GentR). Expresses archaeal ...tyrTV gene replaced by tRNA variant that suppress the TAG nonsense codon, and tyrU gene replaced by tRNA ...tyrS gene knocked out, tyrTV gene replaced by tolC, and tyrU gene replaced by GentR) and release factor 1...biopolymers. Tips for Success Before beginning to reprogram the genetic code, there are several things to ... are true. Always express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian...RF1 function removed, with Ubiquitin-UAG-sfGFP reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP all...removed, ClpS inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 No TCG, TCA, or TAG...

...Selectable markers Viral expression and packaging Reporters, shRNA expression, transgenics and genome...host organism. Host Relevant Promoters Representative Empty Backbones Mammalian CMV, SV40, EF1a, ...plasmid series - Expression with Strep, His or optimized YFP tags for purification...Tag or Fusion Protein Common uses Representative Empty Backbones Flag Epitope tag... Marker Typical Host Organism Representative Empty Backbones Neomycin (G418) ...methods: Delivery method Advantages Representative Empty Backbones Lentiviral Can transduce... AdEasy®-1 plasmid Back to Top Reporters, shRNA expression, transgenics and genome ...

...modifications: replacement of the Cbh promoter with EF1alpha addition of an mCherry reporter variant nuclear...utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate gene targets. To accomplish this ...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...nuclear reporters via an H2B fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide ...fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion for gene activation CRISPR/Cas9...175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR...

...2~3-fold repress on targeted fluorescent genes. These plasmids are described in: Repurposing CRISPR as...Design Tools CRISPR Blog Posts We have recently repurposed the bacterial immune system, CRISPR ( C lustered...pathway, as an RNA-guided DNA binding platform, to repress expression of arbitrary genes in bacteria or human...efficiently block transcription (in bacteria, ~300-fold repression) by interfering with transcriptional elongation...plasmids in bacteria could cause up to 300-fold repress on targeted genes. 2. Two-plasmid CRISPRi system...Lentiviral vector that contains sgRNA targeting repetitive sequence of human MUC4 exon 3 46915 pU6-sgGAL4...

.... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with 1. homology to the DNA flanking ...

.... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...

.... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...

...activity reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based activity reporter of SARS-CoV... also lists several luciferase and fluorescent reporter plasmids that have been used for measuring viral...method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad... termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published information...(Link opens in a new window) CRISPR tools and reporters now available from Stanley Qi's lab. COVID-19 ... Coronavirus Resource Hub COVID-19 SARS-CoV-2 preprints from medRxiv and bioRxiv Elsevier's Novel Coronavirus...

.... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...

...to generate high quality preparations. Following production, all antibody preps are titered and undergo...Addgene does not recommend repeated freeze thaw of antibodies and suggests preparing single-use aliquots before...Innovation Antibody Collection webpage. Antibody preparations developed at IPI may differ from Addgene antibodies...Sanger Sequencing Purified recombinant antibody preps contain low levels of co-purified plasmid from transfection...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR...Next-Generation Sequencing (NGS) to confirm library representation. CRISPR screening experiments require large...whether they are used to knockout, activate or repress target genes. Number of gRNAs - A given CRISPR ... taurus ) Library Size Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type...Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi Libraries 177663, 177664, 177670 Inhibition...A - The protospacers contained in the library represent all 4096 (4 6 ) combinations of 6 nucleotides....Editing Human Wei 3rd 3 53,502 sgRNA Pool for Streptococcus pneumoniae 170432 Knockout S. pneumoniae Veening...

...Mammalian expression vectors for TAL effector repeat arrays assembled using the REAL, REAL-Fast or FLASH... into which DNA fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast or .... All three of these methods yield TAL effector repeat arrays with identical DNA and amino acid sequences...together with the Joung Lab Individual REAL TALE Repeat Plasmids in the Joung Lab REAL Assembly TALEN Kit...Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70 NI RVD A 32287 JDS71 HD RVD C...

...has been used for dynamic tracking of repetitive and non-repetitive genomic loci, as well as chromosome ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using...given region. For example, chromosome-specific repetitive loci can be efficiently visualized in living ... targeted sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering...

...plasmids, the cell may instead repair the break using homology-directed repair (HDR). HDR is much less error-prone...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...the DNA, possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding...