...elongation, RNA polymerase binding, or transcription factor binding. The binding specificity is determined...I nterspaced S hort P alindromic R epeats) pathway, as an RNA-guided DNA binding platform, to repress ... CRISPR interfering system (CRISPRi), works independently of host cellular machineries, requiring only...bacteria ) contains an anhydrotetracycline (aTc)-inducible dcas9 gene on a p15A vector with chloramphenicol...10.1016/j.cell.2013.06.044. PubMed PMID 23849981 . Individual plasmids can be ordered via the links below: ...

...117383-AAV1 TRE-DIO-eYFP An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein...to generate AAV virus that will express light-inducible site-specific iCreV recombinase Hongkui Zeng 140135...to generate AAV virus that will express light-inducible site-specific iCreV recombinase Hongkui Zeng 140136... to generate AAV virus that will expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140136... to generate AAV virus that will expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140137...to generate AAV virus that will express light-inducible site-specific iFlpV recombinase Hongkui Zeng 140137...to generate AAV virus that will express light-inducible site-specific iFlpV recombinase Hongkui Zeng 140552...

...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...recruit a cytidine deaminase and induce somatic hypermutation in a 100 bp window. This technique creates diverse...specific base editor that can alter DNA bases without inducing a DNA break. Base editors convert C->T (or G->... on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert...homology to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID...avoiding known transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter Selectable...

...COVID-19 Plasmid Collection (Link opens in a new window) Researchers developing novel diagnostics, therapeutics...viral proteins as a first step for many different kinds of important experiments. In an effort to speed ...SARS-CoV-2, Ginkgo Bioworks (Link opens in a new window) used their DNA design and synthesis capabilities...plasmids available to scientists in academic and industrial organizations. For more information on ordering...entity, please see our Plasmid Distribution to Industry page. These plasmids have not been expressed or.... Plasmids Use the search bar or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based ...(Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Resources Read Addgene's Blog Synthesized...

...spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity is determined by customizable...TAL Effector Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit... is truncated at both the N and C terminus and induces mutation at rates much higher than the parental...expression in yeast. TALORs consist of the DNA-binding domain of a TALE, along with strong nuclear localisation...TALORs built into this vector under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter...architecture has been shown to increase mutation induction when using TALENs. The FokI domains (DD, RR) used... protein (previously shown to preserve the DNA-binding ability of TALEs), 63 wild-type TAL amino acids...

...ORF. MAPK Nuclear Receptors Find a Pathway or ORF Kit Disease Research Find deposited plasmids for specific...collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics...and depositor collections on one page to help you find what you're looking for. If you are looking for ...based on type or application. Browse our guides to find the plasmids and science information you need before...Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits are groups...Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has established a common...

...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...Systems Biology Lab website (Link opens in a new window) References Botman D, de Groot DH, Schmidt P, Goedhart...019-38913-z. PubMed 30783202 (Link opens in a new window) Lee S, Lim WA, Thorn KS. Improved blue, green,...journal.pone.0067902. PubMed 23844123 (Link opens in a new window) Loqué D, Lalonde S, Looger LL, von Wirén N, Frommer...nature05579. PubMed 17293878 (Link opens in a new window) Sheff MA, Thorn KS. Optimized cassettes for fluorescent.../yea.1130. PubMed 15197731 (Link opens in a new window) Vickers CE, Bydder SF, Zhou Y, Nielsen LK. Dual...2859-12-96. PubMed 24161108 (Link opens in a new window)...

...Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools/controls and tetracycline... Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression of EYFP 1 Gradinaru ...transactivators that can be used with tetracycline (tet)-inducible expression systems . ID Name Expression System...transactivator (tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off...Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression...

...separate plasmid that does so; however, these independent gRNA plasmids can be paired with a wide variety...empty vectors have been deposited at Addgene. To find the gRNA vector for your system, you can search ...Marraffini pgRNA-bacteria 44251 Bacteria SpeI + HindIII none S. pyogenes Qi pDD162 (Peft-3::Cas9 + Empty...pyogenes Joung sgRNA with U6 promoter 48962 Worm HindIII none S. pyogenes de Bono sgRNA with rpr-1 promoter...Qi pRPR1(1xTetO)_gRNA_handle_RPR1t 62966 Yeast HindIII none S. pyogenes Puro Lu pML104 67638 Yeast BclI-SwaI...pyogenes Yang pRPR1_gRNA_handle_RPR1t 49014 Yeast HindIII none S. pyogenes LEU2 Lu pDestTol2CG2-U6:gRNA 63156...60901 Mammalian U6 yes, FokI S. pyogenes Conklin pcrRNA.ind 61284 Bacteria araB none S. pyogenes Beisel pcrRNA.con...

...Link opens in a new window) Pubmed (Link opens in a new window) Article Individual plasmids can be ordered...phsp70-Cas9 and plasmids encoding two chiRNAs can induce defined deletions between the two cleavage sites...contains additional information CRISPR Optimal Target Finder Identifies gRNA target sequences from an input ...input sequence and checks for off-target binding. Currently supports over 20 model and non-model invertebrate...

...log in to your account and check the individual plasmid page to find current pricing information Standard...Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ What Quality Control analyses are performed...website. Every order will need to be completed independently including the check out and will require separate...separate shipping charges. Where can I find cloning resources or scientific support? Please see our Addgene...Addgene Protocols for resources you may find helpful. Our Scientific and Customer Support Teams are also...

...plasmids for precisely editing DNA bases without inducing a DNA break....convert cytidine to uridine within a small editing window near the PAM site. Uridine is subsequently converted...Cas variant and deaminase to adjust the editing window, or adding fusions that increase product purity...base editors are designed to work in a very narrow window proximal to the PAM sequence, some base editing...variants (somatic hypermutation) in a wider editing window, and are thus well suited to directed evolution...Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase (AID). Want more information...

...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->... on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert...avoiding known transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter Selectable...PAM = NGG) 49014 pRPR1_gRNA_ handle_RPR1t pRPR1 HindIII S. cerevisiae LEU2 none, need Cas9 plasmid Lu Do...

...Alias: CN4361) Expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...: CN4035) Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...Alias: CN3987) Expression of jRGECO1a in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs Jonathan Ting Viviana Gradinaru...of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs) Jonathan...of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs) Jonathan...

...opens in a new window) The Michael J. Fox Foundation (MJFF) (Link opens in a new window) encourages researchers... table below. ID Plasmid Gene/Insert Mutations Industry Return to top MJFF Shared Research Tools Labs ...number of Shared Research Tools (Link opens in a new window) , such as CRISPR genome editing tools deposited...table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

...because the Cas9 nickase doesn't have the ability to induce double-stranded breaks like the wildtype Cas9 does...additional 'G'. Where are double strand breaks (DSBs) induced, compared to where the target site sequence (protospacer... colonies, then genotype them, it is possible to find both single-allelic and bi-allelic cells. Single-allelic...double nickase system, with PX335 vector, you need to find two guides for each cleavage site, but this system... we usually pick 3-6 guides around the region to find the most efficient guide (most guides would work...single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing, for example...

... each library can be found on the individual library page. Find Pooled Libraries of Interest Click...pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require electroporation...Knockout Human Sabatini 3rd 10 30,290 Human DNA Binding Domain-Focused CRISPR Knockout Library 123334 Knockout... Mouse Teichmann N/A (retroviral) 5 90,230 RNA-Binding Protein Pooled CRISPR Knockout Library 141438 Knockout...Mouse Sabatini and Lander 3rd 10 188,509 Yeast Inducible CRISPRi Library – Ampl133 161829 Inhibition Yeast...

...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->... on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert...homology to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID...

...guide RNA transcript and a repair template for an individual tagging event. The kit contains 26 plasmids; ...SapTrap CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed... homology arms. Notably, they have used Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate..., Flag, YFP, etc) and selection markers. Two independent selection markers are available. An improved ...available collection of fluorescently tagged, human, induced pluripotent stem cell (hiPSC) lines. These cell...

...promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically used for gRNA...decreases as RNA is degraded within the cell Short window of CRISPR component expression may decrease off-target...Transient expression of CRISPR components Short window of CRISPR activity may decrease off-target effects...PAM sequence is absolutely necessary for Cas9 to bind target DNA. As such, one can start by identifying...DNA, subcloning into a plasmid, and screening individual clones. PCR amplification and next-generation...