...need before starting your experiment. Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs...all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology Pooled...Collection Cell Migration Consortium Center for Genome Engineering EXtracellular Plasmid RESource (EXPRESs... for Solute Carrier Proteins (SLCs) Structural Genomics Consortium Zinc Finger Consortium Pathways & ORFs...

...Zebrafish Genome Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish... zebrafish research. This includes plasmids for genome editing, empty backbones, fluorescent proteins,... Zon Lab Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system - Shuo Lin ...lineage tracing by combinatorial and cumulative genome editing - Jay Shendure Lab Simultaneous single-...fluorescent proteins for tagging genes at endogenous genomic loci in zebrafish. NTR 2.0 - Jeff Mumm Lab. NTR...Database (ZFIN) - A curated database of genetic, genomic, and developmental data for zebrafish biology. .... Seurat - R package for analyzing single cell genomics in zebrafish. Return to top Do you have suggestions...

...specific modification of monocot and dicot genomes using genome engineering; validated in tomato, tobacco...variety of molecular biology technologies: from genome engineering and gene regulation to fluorescent ...biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes - Zhenghe...system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants - Yao-Guang... virus (TRV) that simplifies gene silencing and genome editing logistics - Jose-Antonio Daros. Return ... agriculture and conservation. Medicinal Plant Genomics Resource (Link opens in a new window) - Transcriptomic...

... is a system that can regulate a genome instead of modifying a genome. 1. Two-plasmid CRISPRi system for...works on applying the wild-type CRISPR system for genome mutagenesis ( Cong et al. , Mali et al. , Jiang...custom designed to target arbitrary sequences in the genome. Co-expression of both plasmids in bacteria could...can be custom designed to target sequences in the genome. The pgRNA_humanized also contains a CMV-puro-t2A-mCherry...Cas9 expression plasmid for use editing bacterial genomes 44251 pgRNA-bacteria A customizable gRNA expression...EGFP gene that is stably integrated into HEK293 genome 46922 pSNR52-sgTEF1 Yeast CEN/ARS vector (Ura3)...

...Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1 promoter that...Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent expression of the tTA from the...Gradinaru 99130-AAV1 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV2 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV8 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV9 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAVrg pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...

...Collections Structural Genomics Consortium Plasmids Browse All SGC Plasmids Structural Genomics Consortium Plasmids...Browse plasmids from the Structural Genomics Consortium (SGC) for 3D protein structure studies Plasmid...

... Genome Engineering CRISPR Joung Lab CRISPR Plasmids Joung...endonucleases that can be used to perform targeted genome editing. RGNs consist of two components: a short...variable nts at its 5’ end to base pair with a target genomic DNA sequence and a nuclease (e.g.—the Cas9 endonuclease...www.crispr-cas.org . Users are also encouraged to join the Genome Engineering newsgroup here . Links to additional...

...bacterial genome editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome Engineering...using CRISPR in your lab, specific CRISPR tools for genome editing in bacteria, and more. Synthetic Biology...range of organisms. Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid...opens in a new window) JGI Integrated Microbial Genomes and Microbiomes (IMG/M) (Link opens in a new window...Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window) Return...

...Plasmid Collections Structural Genomics Consortium Plasmids Structural Genomics Consortium Plasmids You may...Plasmids from the Structural Genomics Consortium (SCG), which are primarily used for determining protein...like... David Waugh Lab plasmids The Structural Genomics Consortium is a not-for-profit organization that...

...sequencing of the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final...confirm the identity and integrity of the viral genome and the absence of unexpected DNA contaminants....pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] ...Electron Microscopy The ratio of empty to full (i.e., genome containing) AAV particles within representative...of a lentiviral vector, incubated for 72 h, and genomic DNA is extracted. Primers and probes targeting ...harvesting cells several days later, and isolating genomic DNA. This DNA is then used as a template to amplify...

...exactly match your genomic target? Variation between a given gRNA sequence and your genomic target may reduce...Plasmids: gRNAs Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...gRNA plasmids designed to target various genes or genomic regions. It is highly recommended that you determine...

...gmail.com We have found that a variety of Cas9-mediated genome modifications can be efficiently generated in Drosophila... injection approach, stable lines with targeted genome alterations can be generated within a month. phsp70...invertebrate species. These plasmids are described in: Genome engineering of Drosophila with the CRISPR RNA-guided...homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the...

...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ... Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and...Cas9 (dCas9) can be used to purify a region of genomic DNA and its associated proteins, RNA, and DNA. ...fluorescent protein (FP) can help visualize specific genomic loci using fluorescent microscopy in living cells...sequence to direct the dCas9-FP fusion to a specific genomic sequence - potential target locations can include...

...These viruses also integrate stably into the host genome, enabling long term transgene expression. There...because this virus integrates randomly into the host genome, it has the potential for insertional mutagensis...conjunction with the plasmid that encodes the viral genome (typically called the transfer plasmid ). This ...Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material enclosed in the viral...lentivirus is used for research, it is the lentiviral genome that encodes genetic material that the researcher... wants delivered to specific target cells. This genome is encoded by plasmids called "transfer plasmids...

...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ... Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and...Cas9 (dCas9) can be used to purify a region of genomic DNA and its associated proteins, RNA, and DNA. ...pLtetO-1 Fujii Efficient isolation of specific genomic regions and identification of associated proteins...

...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...Knockout Pooled Library - Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with...

...Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...multiple components) to target locations in the genome defined by a guide RNA. The transposase can then...insertions. CASTs are most often used in bacteria genome editing, though some mammalian systems have been...

... plasmids for CRISPR-Cas and Cascade for large genomic deletions....Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...into a DNA “shredder” system that can cause large genomic deletions. Compared to deletions generated by Cas9...

... and mutations to the bacterial genome itself. Manipulating the genome directly can give you more subtle...Expression Systems Bacterial Expression Systems Genome Engineering Visualization and Tagging Purification... bacterial expression systems to find out more! Genome Engineering Although overexpressing or knocking...there are many different ways to alter bacterial genomes. From recombineering to CRISPR , this collection...variety of tools to help you harness bacterial genomes for your research. Plasmid ID Technique PI Purpose... attachment site found in many enterobacterial genomes. The bacteria of interest are transformed with ...vectors for protein purification from the Structural Genomics Consortium and Nicola Burgess Brown containing...

...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene...