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  1. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...or search the tables below for CRISPR RNA targeting plasmids. Plasmids are available for expression in...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that should ... Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In ...

  2. CRISPR Plasmids for Genomic Visualization

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    Collection
    ... CRISPR Visualize CRISPR Plasmids:...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR...Plasmids: Visualize Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...fluorescent proteins to visualize targeted genomic loci. CRISPR imaging has numerous advantages over other imaging...like fluorescence in situ hybridization (FISH), CRISPR imaging offers a unique method for detecting the...

  3. CRISPR Plasmids - Zebrafish

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    Collection
    ... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...following CRISPR plasmids have been designed for use in zebrafish. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...Zebrafish Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance...

  4. CRISPR Plasmids - Repress Gene Expression

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    Collection
    ... CRISPR Interfere CRISPR Plasmids: Interfere...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...search the tables below for CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression...CRISPR plasmids for transcriptional repression of target genes. CRISPR...Interfere Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...

  5. CRISPR Plasmids - Double-Strand Break (Cut)

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    Collection
    ... CRISPR Cut CRISPR Plasmids: Cut Browse CRISPR...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully...search the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for expression...CRISPR plasmids that create double strand breaks in DNA. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert... elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...

  6. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Activate CRISPR Plasmids: Activate Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...search the tables below for CRISPR plasmids for transcriptional activation. Plasmids are available for expression...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter...Publication C. elegans ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter...

  7. Plant Plasmids and Resources

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    ... Plasmid Collections Plant Plasmids Plant Plasmids and Resources...biology - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general...introduction to CRISPR technology or the Plant CRISPR Resources for a full selection of plasmids expressing...like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription...addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use ...Rice - Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors,...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  8. Microbiology Resources

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    Collection
    ... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene...Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR technology. Check...software. Addgene yeast CRISPR resources Bacterial CRISPR Resources The CRISPR technology was developed...Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid : CRISPR expression...Addgene Microbiology Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence, cloning ...synthetic biology. CRISPR Resources : Find essential background information on using CRISPR in your lab, specific...guide on assembly standards. Browse Plasmids by Microbe System Plasmids for Archaea Species Haloferax sp....

  9. CRISPR Plasmids - Xenopus

    Type
    Collection
    ... CRISPR Xenopus CRISPR Plasmids: Xenopus Browse...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation... The following CRISPR plasmids have been designed for use in Xenopus. Cut Fully functional Cas9 enzymes...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance...

  10. CRISPR Plasmids - C. elegans

    Type
    Collection
    ... CRISPR C. elegans CRISPR Plasmids: C. ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...following CRISPR plasmids have been designed for use in C. elegans. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in C. elegans. CRISPR.... elegans Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will...

  11. CRISPR Plasmids - Base Edit

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    ...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two... or search the tables below for CRISPR base editing plasmids. Plasmids are available for expression in...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  12. CRISPR Plasmids - Drosophila

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    Collection
    ...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR... The following CRISPR plasmids have been designed for use in Drosophila and other insects. Cut Fully functional...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will...

  13. Qi Lab CRISPR Page

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    ... CRISPR Qi Lab CRISPR Plasmids Qi Labs CRISPR Plasmids...Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts We have recently...CRISPR CRISPRi Cas9 gRNA plasmids from the Qi lab CRISPR... both plasmids in bacteria could cause up to 300-fold repress on targeted genes. 2. Two-plasmid CRISPRi...targeted fluorescent genes. These plasmids are described in: Repurposing CRISPR as an RNA-Guided Platform for... 23849981 . Individual plasmids can be ordered via the links below: ID Plasmid Description 44246 pdCas9...fusion expression plasmid 44248 pgRNA-humanized A customizable gRNA expression plasmid for use with either...

  14. CRISPR Plasmids - Mammalian Expression

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    Collection
    ...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR...evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter...

  15. Zebrafish Plasmid Collection

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    Collection
    ... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...highlights plasmids that contain tools for use in zebrafish research. This includes plasmids for genome...and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description Gene...

  16. CRISPR Plasmids - Bacteria

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    Collection
    ... CRISPR Bacteria CRISPR Plasmids: Bacteria...following CRISPR plasmids have been designed for use in bacteria. Cut Fully functional CRISPR/Cas enzymes...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...CRISPR plasmids that have been designed for use in bacteria. CRISPR...opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...

  17. CRISPR Plasmids - Yeast

    Type
    Collection
    ... CRISPR Yeast CRISPR Plasmids: Yeast Browse ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... following CRISPR plasmids have been designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in yeast. CRISPR...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...

  18. Genomic Deletions in Mammalian Cell Lines

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    Collection
    ...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud...usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous... CRISPR References & Information Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol...regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system may be re-purposed...utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP .... Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting CRISPRs into Cells of Interest NOTE: ...: This protocol involves the delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is ...

  19. Jaenisch Lab CRISPR Plasmids

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    ...Jaenisch Lab CRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA... CRISPR Jaenisch Lab CRISPR Plasmids Jaenisch...CRISPR Cas9 gRNA plasmids from Dr. Rudolf Jaenisch's lab. CRISPR...gRNA Design Tools CRISPR Blog Posts CRISPR-on is an RNA-guided transcriptional activator system with two...reaction. These plasmids are described in: Multiplexed activation of endogenous genes by CRISPR-on, an RNA-...Table 2. pmax dCas9 Activator Expression Plasmids ID Plasmid 48223 pAC91-pmax-dCas9VP64 : dCas9VP64 on...Link opens in a new window) Article . Individual plasmids can be ordered via the links below: Table 1. dCas9...

  20. FlyCRISPR

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    Collection
    ... CRISPR flyCRISPR Lab CRISPR Plasmids flyCRISPR Plasmids Available from Addgene You may also...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Harrison Lab - Wildonger...Browse CRISPR Cas9 gRNA plasmids for drosophila research from the O'Connor-Giles, Wildonger, and Harrison...species. These plasmids are described in: Genome engineering of Drosophila with the CRISPR RNA-guided Cas9...window) Article Individual plasmids can be ordered via the links below: ID Plasmid 46294 pBS-Hsp70-Cas9 : ...Gratz, et al. (2013). Plasmid is low copy number. 45946 pU6-BbsI-chiRNA : Plasmid for expression of chiRNA...generating gRNAs 106.5 KB flyCRISPR website contains additional information CRISPR Optimal Target Finder ...
Showing: 21 - 40 of 74 results