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We narrowed to 62 results for: cloning

Showing: 21 - 40 of 62 results

  2. CRISPR Plasmids - Xenopus

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

  3. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

  4. Plan Your Experiment

    Type
    Collection
    ...using standard restriction-ligation cloning. However, the exact cloning strategy will depend on the gRNA ...targeted region from DNA, subcloning into a plasmid, and screening individual clones. PCR amplification and...your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once you have selected your target...sequences it is time to design your gRNA oligos and clone these oligos into your desired vector. In many cases...whether the edit was successful. PCR amplification, subcloning and Sanger sequencing (for HDR or NHEJ): Provides...

  5. Resolute Plasmid Collection

    Type
    Collection
    ...Plasmid Collection You may also like... Gateway Cloning Brain Initiative Collection NeuroMab Collection.... These vectors allow a fast and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors...

  6. CRISPR Plasmids - C. elegans

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

  7. Mammalian RNAi Tools

    Type
    Collection
    ...Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral backbone... Additional Resources Addgene Resources pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral...

  8. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...should be avoided whenever possible. Other popular cloning strategies for the assembly of synthetic parts ...systems such as MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common...

  9. Ras Pathway

    Type
    Collection
    ...Pathway Clone Collection 2.0: Set of vectors from the Esposito Lab for use with the Gateway® Cloning Platform...Pathway Ras Pathway You May Also Like: Ras Pathway Clone Collection 2.0 mTOR Pathway p53 Pathway Background...

  10. CRISPR Plasmids - Drosophila

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

  11. Distribution to Industry

    Type
    Collection
    ... Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining...Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic...

  12. CRISPR Plasmids - Yeast

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed ...

  13. Worm Expression Resources

    Type
    Collection
    ...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified...

  14. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid...Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6...

  16. Synthetic Biology - Strains

    Type
    Collection
    ...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

  17. Tetracycline Inducible Expression

    Type
    Collection
    ...Backbones Find backbones or constructs to use for cloning to control expression of your gene of interest....EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible shRNA expression, with ...Sleeping Beauty transposon system with luciferase in cloning site. See article ( Kowarz et al., 2015 ) for additional... for inducible expression; insert with Gateway cloning and puromycin selection rtTA-Advanced Tight TRE...

  18. Synthetic Biology - Metabolism

    Type
    Collection
    ...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

  19. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed ...
Showing: 21 - 40 of 62 results