...Specific gRNA expression plasmids can be built by cloning a pair of annealed oligonucleotides (each 26 nts...BsmBI-digested MLM3636 vector. The oligonucleotides to be cloned can be designed using the ZiFiT Targeter software...

...Specific gRNA expression plasmids can be built by cloning a pair of annealed oligonucleotides (each 26 nts...BsaI-digested pDR274 vector. The oligonucleotides to be cloned can be designed using the ZiFiT Targeter software...

... for Cloning 67720 Worm NotI/BamHI none S. pyogenes Colaiacovo pRB17 52527 Fly blunt end cloning none ...or nick. Selection , such as Puromycin or EGFP Cloning enzyme used for insertion of your gRNA sequence... by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ...BbsI yes, cut S. pyogenes Puro Zhang sgRNA(MS2) cloning backbone 61424 Mammalian BbsI none S. pyogenes ... U6 yes, cut (enhanced) S. pyogenes Zhang gRNA Cloning Vector Bbs I ver. 2 85586 Mammalian BbsI None S...nickase expression cassette using the Golden Gate cloning method. A separate accessory pack is available ...expressed from independent promoters. sgRNAs are cloned into separate vectors and then assembled into one...

...collection include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription...the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice ...crowd-sourced expression guides . Researchers can clone these enzymes into an expression vector of their...Plasmids Jennifer Molloy Lab Plasmids Golden Gate cloning...

...Pathway Clone Collection 2.0 : Set of vectors from the Esposito Lab for use with the Gateway® Cloning Platform...

...The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et al., Gene, 1992 ), and since...506 517 93 5.7 10 min Monomer pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression Jump to Top Yellow...529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression pCS2+8CmCitrine.../Xenopus/C.elegans/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-N1... 548 562 49 6.5 2.5 hr Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression pCS2+...pmScarlet_C1 - Mammalian Expression pCS2+mScarlet-C Cloning Vector - Mammalian Expression mScarlet-I 569 593...587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry ...

... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

...BsaI digested vector. Cloning targeting chiRNAs Targeting chiRNAs are easily cloned by annealed oligos into...

...Plasmid Collection You may also like... Gateway Cloning Brain Initiative Collection NeuroMab Collection.... These vectors allow a fast and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors...

...Lentiviral vector for inducible expression for Gateway cloning; selection cassette in format: PGK-rtTA-2A-puro...shRNA; see article for detailed information about cloning and additional plasmids tetR-KRAB On Aebischer ...Elledge 35625 pAAV-Ptet-RFP-shR-rtTA AAV; shRNA cloning vector; for evaluation of shRNA efficacy using ...Sleeping Beauty transposon system; has luciferase in cloning site; see article for additional selection and ...

... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

...should be avoided whenever possible. Other popular cloning strategies for the assembly of synthetic parts ...systems such as MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common...

... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

...Pathway Clone Collection 2.0: Set of vectors from the Esposito Lab for use with the Gateway® Cloning Platform...Pathway Ras Pathway You May Also Like: Ras Pathway Clone Collection 2.0 mTOR Pathway p53 Pathway Background...

... gRNA Cloning Vector Bbs I ver. 2 An empty sgRNA expression vector. sgRNA sequence can be cloned into ...Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites. 92221 pLenti_dCas9-2xAM_hIRF-1 ...

... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed ...

...of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid...Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI pU6-pegRNA-GG-acceptor...

...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified...

...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...