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Showing: 21 - 26 of 26 results

  1. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...production, including codon optimization, deletion of the C-terminal 18-21 amino acids and a D614G change which...reporter vector expressing firefly luciferase with neomycin resistance. Other Resources Protocols Bloom lab...

  2. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...expression vector. All His-tags are cloned at the C-terminus of MDH and, if indicated on the plasmid map...Organism or species (e.g., human, watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic...

  3. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources ...and Port pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, cut Ji-Long Liu U6-BbsI-crRNA dU6 BbsI Transfection...

  4. Mammalian RNAi Tools

    Type
    Collection
    .... Rubinson DA, Dillon CP, Kwiatkowski AV, Sievers C, Yang L, Kopinja J, Rooney DL, Zhang M, Ihrig MM, ... 11586 pSico PGK puro Cre addition causes the puromycin gene to be recombined out of the construct, activating...12084 pSicoR PGK Puro Cre addition causes both puromycin and shRNA to be recombined out of the construct...Derivative of pMKO.1 puro with GFP instead of puromycin resistance gene William Hahn Additional Resources...

  5. Church Lab CRISPR Plasmids

    Type
    Collection
    ...involves co- expression of a Cas9 protein bearing a C terminus SV40 nuclear localization signal with one...plasmids is described in: Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Dicarlo...meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin 48668 M-SPcas Mammalian S. pyogenes Cas9 expression...pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin 48647 DS-ST1cas Bacterial S. thermophilus #1...#1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin 48648 DS-TDcas Bacterial T. denticola Cas9 (... (TD) + tracrRNA expression, cloDF13/spectinomycin 48649 PM-SP!TA Bacterial SP crRNA expression: targets...

  6. Control AAV Preps

    Type
    Collection
    ...Constitutive 2 Patrick 105922 pAAV-CBh-mKate2-IRES-MCS (C-gap-WT) CBh mKate2 Constitutive 2 Patrick 114469 pAAV-CaMKIIa-mCherry...dependent 8 Deisseroth 98927 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 CAG GFPsm_myc (does not fluoresce) Cre ...
Showing: 21 - 26 of 26 results