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Showing: 21 - 40 of 93 results

  1. Brain Initiative Collection

    Type
    Collection
    ...Addgene distributes ready-to-use viral preps made from plasmids in our collection! AAV are distributed as ...NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection . The NeuroMab Facility and James Trimmer have produced...Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven KOR DREADD...recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use recombinant antibodies made from...that are currently in our repository. AAV are distributed as 100 µL of purified viral particles in solution...

  2. Viral Production

    Type
    Collection
    ...subjected to quality control by Addgene before being distributed to customers. Details about our production protocols...control are described below. AAV Production AAV distributed by Addgene has been produced either in-house ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...containing lentivirus is first cleared by low-speed centrifugation and then by filtration through a 0.45 µm polyethersulfone...

  3. Luciferase Plasmid Collection

    Type
    Collection
    ...secreted version of the protein is available – these attributes have made it popular with scientists since its...enchancer regions in Drosopholia Herman Wijnen 126033 pTETRIS-cargo Firefly For use as a reporter of the ability... in plants, co-expressed with mScarlet-I for ratiometric imaging David Nelson 141301 pRATIO1267 RedLuc...plants, co-expressed with Gaussia luciferase for ratiometric imaging David Nelson 212935 pGL4.84(hRlucCP/Puro...Lentiviral expression of firefly luciferase Brent Stringer 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral...

  4. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...101781 CETN2-mTagRFP-T AICSDP-22 mTagRFP-T Centrin-2 Centrioles 101782 LAMP1-mEGFP AICSDP-19 mEGFP LAMP-...lines have been deposited here with Addgene for distribution to the research community. With these plasmids...Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions 87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin...

  5. AAV Packaged on Request

    Type
    Collection
    ...amplification Viral vector production Density centrifugation purification Addgene’s comprehensive quality... and results through Addgene’s Data Hub . By contributing your AAV vector experience, from methods to ...titration, and quality control measures. We have distributed preps around the globe and shared our well-developed...lab, and even managing legal permissions and distribution. Troubleshooting If your answer isn’t in the...

  6. p53 Pathway

    Type
    Collection
    ...candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2 SESN3 Sestrins 1, 2, or 3 Siah Siah E3 ubiquitin...stresses like DNA damage, oxidative stress, and nutrient deprivation. p53 is activated through both phosphorylation...ribonucleotide reductase M2 B (RRM2B) PAG608 Zinc finger, matrin-type 3 PAI Serpin peptidase inhibitor, clade E ...

  7. Fluorescent Protein Guide: FRET

    Type
    Collection
    ... super-REACh via a 10 amino acid linker ACA Heterotrimeric construct consisting of Amber-5aa linker-Cerulean...linker-Cerulean-6aa linker-Amber ACV Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa linker-Venus...linker-Venus VCA Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa linker-Amber VCV ...VCV Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa linker-Venus ACAV Heterotetrameric...

  8. Open Enzyme Collection

    Type
    Collection
    ...Enzyme Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...polymerases, DNA ligases, reverse transcriptases, and restriction enzymes. These enzymes are useful in both basic...the links below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases...-phi29pol phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert ...

  9. Cre-lox system

    Type
    Collection
    ...a promoterless vector with several available restriction sites none Mammalian Sauer 11957 pBS595 tet-hCMV-EGFPcre...Muscle-specific expression of Cre MCK Mammalian Kahn 13763 pTriEx-HTNC Cre fused to HIV-TAT - promotes cellular uptake...puro Luciferase - EGFP- Cre fusion CAG Mammalian Stringer 68448 TOPO rtTA3-2A-Cre iRFP670, Cre, and GFP ...Luo 119063 pBac(3xP3-gTc’v; Tc-hsp68:Cre) Cre Tribolium casteneum hsp68 T. castaneum (red flour beetle...Ohlmann 120279 014LP-pAAV-ANF-657- Cre-pA Cre; for atrial-specific expression ANF AAV Wehrens 121675 pAAV-EF1a-fDIO-Cre... partnership with genOway, Addgene is able to distribute materials containing FLEx technology. Read the...

  10. Plan Your Experiment

    Type
    Collection
    ... containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact cloning ...agarose gel to identify DNA fragments. PCR and restriction digest (for HDR repaired DSBs): For small nucleotide...nucleotide edits that introduce a novel restriction site. The region of interest is PCR amplified, digested...digested with the appropriate restriction enzyme and run on an agarose gel to identify DNA fragments. PCR...

  11. Bacterial Expression Systems

    Type
    Collection
    ...protein resources here . pCyPet-His 14030 FRET Patrick Daugherty FRET: Vector for arabinose inducible ...YPet) found in pYPet-His . pYPet-His 14031 FRET Patrick Daugherty FRET: Vector for arabinose inducible ... and P. pastoris . All plasmids use the same restriction enzymes for cloning so multiple constructs can...compatible with standard cloning using many different restriction enzymes. pPro24-gfp contains GFP but all others...

  12. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...Plasmids for REAL (Restriction Enzyme And Ligation) TALEN assembly containing single TALE repeat domains...any desired sequence, referred to as the REAL (Restriction Enzyme And Ligation) assembly method ( Sander...encoding a single TALE repeat domain and flanked by restriction sites used for ligation into longer arrays. These...

  13. Plasmids for Stem Cell Research

    Type
    Collection
    ...reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming...conversion of mouse fibroblasts to self-renewing, tripotent neural precursor cells. Proc Natl Acad Sci U S...Spiny Neurons Lentiviral Human Generation of Human Striatal Neurons by MicroRNA-Dependent Direct Conversion.... 2017 Aug;9(8):1117-1131. Parmar Fibroblasts Dentritic Cells Lentiviral Mouse Direct reprogramming of...

  14. AAV Viral Preps

    Type
    Collection
    ... viral vector preps are purified by density centrifugation. Addgene’s comprehensive high-quality quality...genome sequencing (VGS). In-stock AAV preps are distributed as 100 µL solutions of purified viral particles...Samples Small samples of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 8, 9 and retrograde...

  15. CRISPR Guide

    Type
    Collection
    ...activity with endogenous cell-type specific miRNAs to restrict CRISPR editing only to the target tissue. Browse... Acidaminococcus sp. ). The popularity can be attributed to Cas12a’s many benefits. Cas12a exhibits increased... quantification and can be used on lateral flow strips. Other Cas enzymes can be added to SHERLOCKv2 to...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...924–927. PMID: 30377362 Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin... (12), 770–788. PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S....152 (5), 1173–1183. PMID: 23452860 Savic, D., Partridge, E. C., Newberry, K. M., Smith, S. B., Meadows...

  16. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...as in-stock preps. These high-titer preps are distributed as 100 µL solutions of purified viral particles...Vector Core have been transferred to Addgene for distribution. Look up the IDs of particular Penn Vector Core... have the right to use the original plasmid. Distribution of the original plasmid is important because...

  17. Microbiology Resources

    Type
    Collection
    ...microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute self-replicating...Addgene continues to monitor its library and distributions in accordance with export laws and biosafety...Bordetella pertussis Campylobacter sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. ...

  18. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... Other/Tribolium castaneum BsaI none S. pyogenes Averof p(U6b-BsaI-gRNA) 65956 Other/Tribolium castaneum...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations... pKS diaCas9_sgRNA 74923 Other/Phaeodactylum tricornutum U6 yes, cut S. pyogenes Winge pJJR50 75026 Worm...

  19. COVID-19 Resources

    Type
    Collection
    ...Addgene has partnered with Ginkgo Bioworks to distribute SARS-CoV-2 plasmids. The collection contains:... a for-profit entity, please see our Plasmid Distribution to Industry page. The list of plasmids available...Cathepsin L - a cysteine endosomal protease that triggers proteolysis of SARS-CoV Spike protein, activating...

  20. Deisseroth INTRSECT Collection

    Type
    Collection
    ...TVA-mCherry Cre AND Flp pAAV-nEF-Con/Fon OG Cre AND Flp Triple recombinase-dependent Addgene ID Plasmid Logic ... evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons. Elife. 7. pii: e39786... Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal Circuits through the Parafascicular Nucleus. Neuron...
Showing: 21 - 40 of 93 results