Skip to main content
Addgene
Showing: 21 - 40 of 110 results

  1. Qi Lab CRISPR Page

    Type
    Collection
    ...CRISPR interfering system (CRISPRi), works independently of host cellular machineries, requiring only a...transcription (in bacteria, ~300-fold repression) by interfering with transcriptional elongation, RNA polymerase... the gene expression level without genetically altering the target loci. Thus, CRISPRi is a system that...

  2. Plant Plasmids and Resources

    Type
    Collection
    ... molecular biology technologies: from genome engineering and gene regulation to fluorescent proteins. ...modification of monocot and dicot genomes using genome engineering; validated in tomato, tobacco, M. truncatula ...for chloroplast transformation and metabolic engineering in plants (including tobacco and potato) - Scott...microRNA and siRNA vectors in Arabidopsis - James Carrington. JoinTRV , an improved vector system based on...

  3. TALEN Plasmids and Kits

    Type
    Collection
    ...Genome Engineering TALENs TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering Guide...Kits TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL...Voytas Adam Bogdanove Joung Lab TAL Effector Engineering Reagents Assembly via serial ligation. Validated...

  4. COVID-19 Resources

    Type
    Collection
    ...fluorescent reporter plasmids that have been used for measuring viral entry and activity in neutralization assays...Industry column below. For more information on ordering from a for-profit entity, please see our Plasmid...access to articles related to SARS-CoV-2 research during the current outbreak: Cell Press Coronavirus Resource...Collection Science Journals Coronavirus Collection Springer Nature SARS-CoV-2 and COVID-19 Articles Wiley ...

  5. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR...wildtype or double nickase for my CRISPR genome engineering experiments? When assessing which nickase type...type to use for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized ...instructions on designing and using Cas9-based genome engineering tools to perform different types of genome targeting...

  6. Zhang Lab CRISPR Page

    Type
    Collection
    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR... the CRISPR-Cas9 system can be implemented by delivering Cre and sgRNA to a Cre-dependent mouse or sgRNA... References SpCas9 plasmids Multiplex Genome Engineering using CRISPR/Cas Systems. Cong L, Ran FA, Cox.... 2013 Oct 10;155(2):479-80. PubMed . Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD,...

  7. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...be found on the TALengineering website. Users may also wish to join the TALengineering newsgroup . ID ... TALengineering Reagents TALE Repeat Plasmids Individual REAL...

  8. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ... Genome Engineering CRISPR Joung Lab CRISPR Plasmids Joung Lab CRISPR-Cas...RNA-Guided Nucleases ( RGNs ), based on naturally occurring Type II CRISPR-Cas systems, are programmable endonucleases...Users are also encouraged to join the Genome Engineering newsgroup here . Links to additional pages describing...

  9. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... (PBS) and the desired edits on an RT template. During prime editing, the pegRNA directs the Cas9 nickase...leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components have ... improve the efficiency of editing, including exploring new sources for the reverse transcriptase. Many...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Kalle Gehring 45970 pGEX-parkin 77-465 PRKN GST tac Parkinson's Kalle Gehring 45971 pGEX-parkin...Parkinson's Kalle Gehring 45972 pGEX-parkin 219-465 PRKN GST tac Parkinson's Kalle Gehring 45973 pGEX-parkin...Parkinson's Kalle Gehring 45974 pGEX-parkin W403A PRKN GST tac Parkinson's Kalle Gehring 45975 pGEX-parkin...Parkinson's Kalle Gehring 45976 pGEX-parkin C431S PRKN GST tac Parkinson's Kalle Gehring 45977 pGEX-parkin...pGEX-parkin W403A-C431S PRKN GST tac Parkinson's Kalle Gehring 46904 pRK5-EGFP-Tau MAPT GFP CMV Parkinson's, FTD...eIF4G delN83 EIF4G1 V5, MS2 CMV Parkinson's Niels Gehring 66818 pCCL-PGK-SPdCas9-BFP-DNMT1 DNMT1 PGK Hereditary... 86-130 Parkin) PRKN GST tac Parkinson's Kalle Gehring 102361 pcDNA3.1-hSNCA-NE SNCA NE CMV Parkinson'...

  11. Viral Vectors

    Type
    Collection
    ...viruses with modified viral genomes. Naturally occurring viruses harbor genetic material (termed the viral...alternate (non-virus-producing) genomes, naturally occurring viral genomes have been adapted into a plasmid-based...ability to propagate in the same way that naturally occurring viruses do. Instead, the genes that are required...

  12. Zebrafish Plasmid Collection

    Type
    Collection
    ...Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration - Periklis Pantazis...backbone-based Gateway destination vectors (pGCS), bearing either amino- or carboxyl-terminal tags, including...zfRegeneration - A database for gene expression profiling during regeneration. Seurat - R package for analyzing ...

  13. Rett Syndrome

    Type
    Collection
    ...regardless of age or symptom severity, suggesting that restoring MECP2 expression in humans could be curative. ...and repository, and CRO for centralized behavior scoring (Link opens in a new window) MMRRC Repository -...opens in a new window) Protocols.io - protocol sharing site Funding Opportunities (Link opens in a new...

  14. Plan Your Experiment

    Type
    Collection
    ...sites are called off-targets and should be examined during gRNA design. In general, off-target sites are not...gRNAs that have been used successfully in genome engineering experiments. Using validated gRNAs can save your...Publications Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9 system. Methods in Molecular...

  15. Bacterial Expression Systems

    Type
    Collection
    ... Systems Bacterial Expression Systems Genome Engineering Visualization and Tagging Purification Controlled... expression systems to find out more! Genome Engineering Although overexpressing or knocking down a gene...different ways to alter bacterial genomes. From recombineering to CRISPR , this collection contains a variety...

  16. Luciferase Plasmid Collection

    Type
    Collection
    ...gene.. TREAT (3( T hree)′- R NA E nd A ccumulation during T urnover) : Single-molecule mRNA turnover biosensor...Lentiviral expression of firefly luciferase Brent Stringer 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral... Insect expression of firefly luciferase Kevin Maringer 131860 pZmUBQ:LUC Firefly CaMV Zea mays expression...

  17. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks &...Regulation. The collection includes both naturally ocurring and synthetic regulatory elements, pre-assembled...

  18. TALEN Expression Vectors

    Type
    Collection
    ... TALengineering Reagents TALEN Expression Vectors TALEN Expression... FLASH assembly methods can be found on the TALengineering website . All three of these methods yield ...

  19. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...researchers at Stanford University’s Department of Bioengineering, to build DNA that will enable researchers ...industrial organizations. For more information on ordering from a for-profit entity, please see our Plasmid...

  20. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...targeted to a site with a spacer of 7 bps and harboring a “+” heterodimeric FokI domain ( Miller et al...targeted to a site with a spacer of 7 bps and harboring a “-” heterodimeric FokI domain ( Miller et al...
Showing: 21 - 40 of 110 results