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Showing: 21 - 40 of 40 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ...inducible expression of shRNA; neomycin selection; plasmid 21915 has puromycin selection TetR On Wiederschain...from pCW57.1. rtTA was replaced with tTA, and Puromycin was replaced with Blasticidin selection. Please...

  2. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459 ). After you...

  3. Retrovirus Plasmids

    Type
    Collection
    ...pBABE-puro MoMLV For transgene expression, with Puromycin selection (for additional pBabe plasmids, browse... For cloning and gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...

  4. Control AAV Preps

    Type
    Collection
    ...Spaghetti Monster tags 98927 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 CAG GFPsm_myc (does not fluoresce) Cre ...

  5. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...repeat. A retroviral expression vector with the puromycin resistance gene 63591 3xFN-Tel-TAL/pMXs-neo Expresses...repeat. A retroviral expression vector with the neomycin resistance gene 64325 3xFLAG-dCas9/p-bacteria ...

  6. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Addgene plasmid ID 48139), which include GFP and puromycin as selectable markers, respectively, or constructs...consists of DMEM supplemented with 2% penicillin/ streptomycin and 1% L-glutamine, which is used for MEL cells...

  7. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Loren Looger AV-1-PV3661 98927-AAV1 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 Loren Looger AV-10-PV0101 105530...Boyden AV-1-PV3511 98926-AAV1 pAAV.CAG.GFPsm-myc.WPRE.SV40 Loren Looger AV-1-ALL856 100048-AAV1 pAAV.CAG.LSL.tdTomato...

  9. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...reporter vector expressing firefly luciferase with neomycin resistance. Other Resources Protocols Bloom lab...

  11. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...and Port pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, cut Ji-Long Liu U6-BbsI-crRNA dU6 BbsI Transfection...

  12. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen pPBT-PE2-PuroTK-pegRNA_GG...

  13. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...PAM = NGG) pCRISPR BsaI E. coli, S. pneumoniae Kanamycin none, need Cas9 plasmid Marraffini pCas9 BsaI ...

  14. CRISPR Plasmids - Plants

    Type
    Collection
    ...1 none As Cpf1 Qi pKEE401 yes, cut S. pyogenes Neomycin Chen Do you have suggestions for other plasmids...

  15. Ras Pathway

    Type
    Collection
    ...protein, LST8 homolog MTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 NFE2L2 Nuclear factor, erythroid...

  16. CRISPR References and Information

    Type
    Collection
    ...cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2...

  17. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGGAAGCGGACGCGAGTCaagtcacctctggaagCCTGAACGATTCGCAACA mycn TAL3124 & TAL3125 TGCAGCCGTGTTTCTACCcggacgaggatgacttCTACTTCTGCAGACCAGA...

  20. Validated gRNA Sequences

    Type
    Collection
    ...GTTCCGCGTTACATAACTTA 50927 S. pyogenes 24346702 Wolfe Neomycin TCATGGCTGATGCAATGCGG 67594 cut S. pyogenes 26178787...
Showing: 21 - 40 of 40 results