Skip to main content
Addgene
Showing: 21 - 40 of 56 results

  1. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ... or 20 matching nucleotides, the second binds to mcherry with either 0, 11, 18, or 20 matching nucleotides...possible to study the effect of the amount and stoichiometry of enzymes. The pLC97 plasmid allows for quick...

  2. Antibody Plasmid Collection

    Type
    Collection
    ...Fab fragment libraries from chickens Methods for the generation of chicken monoclonal antibody fragments...antibodies as Fab' fragments, isotype-switched chimeric antibodies, and Fc-silent mutants. Functional ...

  3. AAVED

    Type
    Collection
    ...using combinations of AAV-based molecular tools to achieve targeted neuronal manipulation (aka intersectional...Potential combination with other technologies to achieve different experimental goals. How does this method... (that your group is presenting on) is used to achieve targeted neuronal manipulation. Figures, tables...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Lynda Chin 21691 pLenti6/V5/DEST-VPS35 VPS35 V5 CMV Parkinson's Lynda Chin 21827 TLS 1: hTLS.pBSKS...Gabriele Kaminski Schierle 108866 pT7-7 aSyn C141 SNCA T7 Parkinson's Gabriele Kaminski Schierle 108867 pET29b...Kaminski Schierle 108868 pLJM1 EGFP-Tau MAPT GFP CMV Parkinson's, FTD Gabriele Kaminski Schierle 109321 ... mScarlet CMV ALS Kyoko Chiba 184826 pSN838 KIF5A mScarlet CMV ALS Kyoko Chiba 184827 pSN526 KIF5A mScarlet...Strep polH ALS Kyoko Chiba 184832 pSN840 KIF5A mScarlet Strep polH ALS Kyoko Chiba 185714 pAAV-hSyn-GFP-IRES-mSNCA...pMXs-mPKR-FLAG-IG EIF2AK2 Flag LTR VWM disease Seiichi Oyadomari 101848 pTO_CHMP2B_LAP CHMP2B LAP CMV ...p426-Cup1p-FUS-FusionRed FUS FusionRed CUP1 ALS Michihiko Kataoka 188460 pKDR-Lenti-CBh.6-SOD1 SOD1 CBh ...

  5. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...-Puro 73039 Expresses Nluc and copGFP from the chicken beta-actin CMV enhancer pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro... the EF1 promoter while pICPIS-CB contains the Chicken-beta actin promoter with CMV enhancer. pAdx-CMV-LacZ...polyA pICPIS-CB 73356 AdenoX shuttle vector with chicken-beta actin CMV enhancer promoter SV40 polyA...

  6. Viral Production

    Type
    Collection
    ...ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR method. Titering by the...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...Transfer Plasmid Addgene uses a rigorous barcode matching system that follows the sample from the DNA tube...

  7. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... in: A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation... in: Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon ... described in: Expanding the genetic code of Escherichia coli with phosphoserine. Park HS, Hohn MJ, Umehara...

  8. TALEN Plasmids and Kits

    Type
    Collection
    ...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction...is driven by the strong CAG promoter or can be achieved by in vitro mRNA synthesis from the T7 promoter...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

  9. Luciferase Plasmids

    Type
    Collection
    ...sensitivity, wide dynamic range, and resistance to photobleaching has made luciferase a common choice in assays... of renilla luciferase Reuben Shaw 48688 pUltra-Chili-Luc Firefly Ubc Lentiviral expression of firefly... Mammalian expression of firefly luciferase Ming-Chih Lai 115366 p CIneo-RL Renilla CMV Mammalian expression...

  10. Synthetic Biology - Overview

    Type
    Collection
    ...Resources iGEM - International Genetically Engineered Machine Foundation Registry of Standard Biological Parts...synthetic biologists SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource EraSynBio...

  11. Chemogenetics AAV Preps

    Type
    Collection
    ...none 2, 5 Richie 121539 pAAV SYN1 HA-hM3D(Gq) hM3D(Gq) - Activation HA fusion none 2, 5 Richie 50467 pAAV-CaMKIIa-HA-hM4D...

  12. Qi Lab CRISPR Page

    Type
    Collection
    ...CRISPRi), works independently of host cellular machineries, requiring only a nuclease-deficient Cas9 (dCas9...binding specificity is determined jointly by 20-bp matching region on the sgRNA and a short DNA motif (protospacer...

  13. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate...molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun. 2013...

  14. Viral Vectors

    Type
    Collection
    ...scientific review of our viral pages, we thank Boston Children's Hospital Viral Core . Viral Plasmid Biosafety...Au S, Panté N. How viruses access the nucleus. Biochim Biophys Acta. 2011 Sep;1813(9):1634-45. doi: 10.1016...

  15. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate...using streptavidin affinity purification. For both enCHIP and CAPTURE, design your gRNA sequence to direct...

  16. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...strategy relies on cellular delivery of a pair of chimeric single guide RNAs (sgRNAs) to create two double...of PCR products. Finney M, Nisson PE, Rashtchian A. Current Protocols in Molecular Biology. 2001...

  17. Bacterial Expression Systems

    Type
    Collection
    ...protein reporter genes in Gram-negative bacteria. Hochschild Bacterial Two-Hybrid System Various Plasmids ...with lambda-C1 operator Beta-galactosidase Ann Hochschild Bacterial two-hybrid assay using pBRα-β flap ...

  18. Plasmids for Stem Cell Research

    Type
    Collection
    ... Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged...reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming...

  19. Biosensor AAV Preps

    Type
    Collection
    ...Constitutive 9 Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Patriarchi Oxytocin (OT...

  20. TALEN Engineering

    Type
    Collection
    ...assemble TAL effector repeat arrays on a single architectural framework and the DNAs produced by all three...
Showing: 21 - 40 of 56 results