...gently touching the screen. Prepare the Serial Dilution Place a 48-well dilution plate in a chilled 96-well...nothing falls into the plate. Leave dilutions on the chilled 96-well freezer block in the BSC until ready to...Prepare the Master Mix Place a ddPCR plate onto a chilled 96-well freezer block and set aside in the droplet...before use. Place an 8-well PCR tube strip into a chilled 96-well freezer block. Add 20 µL of the master ...aspirating the droplets. Transfer the droplets to a prechilled PCR plate. Pro-Tip To ensure that the droplets...keeping all reagents and samples on ice or pre-chilled 96-well freezer blocks during use. In this protocol...

...amplification of CRISPR pooled plasmid libraries in Escherichia coli cells....amplification of a pooled-plasmid library in Escherichia coli cells. Pooled libraries contain tens to ...Prewarm 8X LB Agar + Antibiotic Bioassay plates. Prechill Micropulser cuvettes on ice. Thaw 4 tubes of electrocompetent...ice for 15-20 minutes or until completely thawed. Chill a box of 200 µL micropipette tips in a -20 °C freezer...kV, 1 pulse. Aliquot 25 µL DNA-Endura into pre-chilled cuvette. Pulse. Caution Electroporation involves...sterile LB at 4 ℃. Day 2 (morning) Before beginning, prechill at least four conical tubes on ice and ensure ...

... order primers that are 60 bp long, with 30 bp matching the end of the adjacent fragment and 30 bp annealing...the assembled parts. Tips and Troubleshooting “Stitching” fragments together using oligos When you need...Pro-Tip Please note that the way to design the “stitching” primers and the amounts of primers to include...TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. (2009). Enzymatic assembly of DNA...Link opens in a new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). Chemical synthesis...

... gently touching the screen. Preparation of the Master Mix Place a ddPCR plate onto a chilled 96-well ...before use. Place an 8-well PCR tube strip into a chilled 96-well freezer block. Add 16 µL of the master ...pipetting the droplets. Transfer the droplets to a prechilled PCR plate. Pro-Tip To ensure that the droplets...keeping all reagents and samples on ice or pre-chilled 96-well freezer blocks during use. To reduce the...

...2 O 0.2 μL Taq DNA Polymerase (5 units/μL) PCR Machine Agarose Gel Procedure Primer Design and PCR Design...added to a master mix. Place reaction tubes in PCR machine. Set annealing temperature 5°C below the primer...liquid to the bottom before placing it in the PCR machine. Diluting Primers Most people order primers from...

...minutes at 95°C in a PCR machine or in a beaker of boiling water. If using a PCR machine, incubate the sample... that does not contain antibiotics. h. Without touching the sides of the dish, gently add DNA:FuGENE® ...PubMed . Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Zufferey R et...

...image and the resolution your microscope can achieve. You achieve the highest resolution when your image is...

...surface and should be handled carefully. Avoid touching the cells when replacing media. T-175 flask, Corning...plasmids, we can determine the total μg/bp we need to achieve a 1:1:1 molar ratio of each plasmid: 2000 μg / ...

...Blanking' measures the background inherent to the machine and your solvent. If using a NanoDrop to measure...

... allow enough time for the loop to cool before touching it to the bacteria. Gently spread the bacteria...

...possible. You will want nice crisp bands. This can be achieved by using a wider gel comb and running the gel ...

...necessary to get to 50μL. Efficient annealing can be achieved by one of two methods: Method #1 Place the mixed...

...can also be placed in walk-in refrigerators to achieve a temperature between 4°C and room temperature....

...separate different components in a liquid sample. It achieves this by using centrifugal force, which is generated...

...submerge the pipette itself into the liquid and avoid touching the pipette to the inside of the tube. Only the...

...antibiotic, which may not be stable at 37 °C. To achieve a stable cell pool, the antibiotic selection should...

... (Link opens in a new window) Chomczynski P and Sacchi N, 2006 and (Link opens in a new window) TRIzol...