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Showing: 21 - 40 of 83 results

  1. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...via use of CRISPR-Cas9. Wu Y, Liang D, Wang Y, Bai M, Tang W, Bao S, Yan Z, Li D, Li J. Cell Stem...cells are used for DNA extraction, although a wide range of cell numbers is acceptable. The bulk sorted cells..., mini-prep each one, and subject each clone to Sanger sequencing to characterize each deletion allele...Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nature Protocols . 2013. PubMed . Characterization...colonies using the Qiagen Miniprep Kit. Zhang S, Cahalan MD. J. Vis. Exp . 2007. PubMed . Transformation...

  2. Bacterial Expression Systems

    Type
    Collection
    ...that selection is not required. pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning and expression of ...optimized FokI endonuclease domain. pET-Sangamo-His 40787 TALEN Gang Bao Gateway cloning and expression of...Various Plasmids Blue/UV , Cyan , Green , Yellow , Orange , Red , Far-Red , Near-Infrared , Large Stokes ...dissect an individual protein’s function under a wide range of highly controlled conditions in vitro , or, if..., Luciferase (LuxCDABE) Attila Karsi Broad host range vectors for expressing luciferase and fluorescent...

  3. Mammalian RNAi Tools

    Type
    Collection
    ...Dillon CP, Kwiatkowski AV, Sievers C, Yang L, Kopinja J, Rooney DL, Zhang M, Ihrig MM, McManus MT, Gertler ...expression; Designed for inducing RNAi in a wide range of cell types, tissues and organisms Luk Parijs ...assessment of gene knockdown. Moore CB, Guthrie EH, Huang MT, Taxman DJ. Methods Mol Biol. 2010. 629:141-58...viral high-content screen. Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, Piqani B, Eisenhaure...

  4. CRISPR Plasmids - Plants

    Type
    Collection
    ...function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates...51295 pRGEB31 rice snoRNA U3 BsaI none S. pyogenes Yang 50579 pBUN6A11 OsU3 BsaI yes, activate S. pyogenes...pRGEB32 rice snoRNA U3 BsaI yes, cut S. pyogenes Hygro Yang 62201 pHSE401 AtU6-26 yes, cut S. pyogenes Hyg Chen...pRGE32 rice snoRNA U3 BsaI yes, cut S. pyogenes Hygro Yang 62202 pKSE401 AtU6-26 yes, cut S. pyogenes Kan Chen...50929 pRGE31 rice snoRNA U3 BsaI none S. pyogenes Yang 47024 pUC gRNA Shuttle Mt U6.6 inFusion none S. ...

  5. Ras Pathway

    Type
    Collection
    ... protein 35 ARHGEF2 Rho/Rac guanine nucleotide exchange factor 2 CCND CCND1 CCND2 CCND3 Cyclin D CDK CDK4...Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 PRKA PRKAA1 PRKAA2 PRKAB1 PRKAB2 PRKAG1... RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange factor RAS KRAS MRAS NRAS Kirsten rat sarcoma ...protein SOS SOS1 SOS2 SOS Ras/Rho guanine nucleotide exchange factor SPRED SPRED1 SPRED2 SPRED3 Sprouty related...Tuberous sclerosis VAV1 Vav guanine nucleotide exchange factor 1 YAP1 Yes associated protein 1 Return ...

  6. COVID-19 Resources

    Type
    Collection
    ...receptor for SARS-CoV-2 and SARS-CoV is hACE2, an angiotensin receptor. Another key step is the priming of ...the links and references below: ACE2 - (Human Angiotensin Converting Enzyme 2) is the host cell receptor...nucleic acids with SHERLOCK and DETECTR . Feng Zhang's lab at the Broad Institute has utilized the natural...design resources (Link opens in a new window) Find Zhang Lab Cas13a plasmids Additional protocol for using...Medicine The proximal origin of SARS-CoV-2 . BSG - Wang, K et al, 2020, BioRxiv SARS-CoV-2 invades host ...

  7. Plasmids for Stem Cell Research

    Type
    Collection
    ...into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fetal Lung Fibroblasts Cholinergic Neurons Lentiviral...into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fibroblasts Oligodendrocyte Precursor Cells Lentiviral...ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult Dermal Fibroblasts Neurons Lentiviral Human... Cell. 2019 Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion...Disease in Mice. Cell. 2020 Apr 30;181(3):590-603.e16. Yang iPSCs Bipolar Neurons Lentiviral Human Rapid neurogenesis...

  8. TALEN Guide

    Type
    Collection
    ...targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove.... A third kit, a "TALe Toolbox", from Dr. Feng Zhang’s group at the Broad Institute, will be available...available from Addgene before the end of the year. Dr. Zhang ‘s lab published a paper on TAL effectors in Nature...

  9. Lentiviral Prep Service

    Type
    Collection
    ...resistance from EFS promoter. Lentiviral backbone. Zhang Cas9 and Accessories for Activating Gene Expression...none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) ...marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang 61426 lenti MS2-P65-HSF1_Hygro MS2-P65-HSF1 Hygromycin...resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI...

  10. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...EGFP Carol Otey 31923 pPaxillin-PSmOrange Focal Adhesions paxillin PSmOrange Vladislav Verkhusha 79999 pZyxin-miRFP703...pEGFP-C1-Fibrillarin Nucleolus Fibrillarin EGFP Sui Huang 36207 pmTurquoise2-H2A Nucleus H2A mTurquoise2 Dorus...Clathrin-coated vesicles Clathrin light chain GFP Bo Huang 54040 mEmerald-Clathrin-15 Clathrin-coated vesicles...vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437 mRFP-Rab5 Early endosomes RAB5A mRFP Ari ...

  11. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...accomplish this they have taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid...desalted oligonucleotides with the appropriate overhangs: The plasmids listed here are provided as empty...Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual ...

  12. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...through base excision repair, creating a C to T change (or a G to A on the opposite strand). Likewise,...guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature,...targeted AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab , in which ...

  13. Jaenisch Lab CRISPR Plasmids

    Type
    Collection
    ...activator system. Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon...

  14. Adenovirus Plasmids

    Type
    Collection
    ...Additionally, these viruses are able to target a broad range of dividing and non-dividing cell types with almost...Gateway cassette; See article for additional plasmids Zhang 50957 RedTrackCMV Shuttle For production of mRFP-trackable...tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular inserts...

  15. Antibody Production

    Type
    Collection
    ...affinity chromatography, antibodies undergo a buffer exchange using centrifugal columns. The final formulation...the protein size is confirmed. Residual Plasmid Sanger Sequencing Purified recombinant antibody preps ... The PCR product is purified, sequenced via the Sanger method, and aligned with the parental plasmid sequence...

  16. Microbiology Resources

    Type
    Collection
    ...Addgene’s repository contains plasmid tools for a range of microbial fields, including bacteria, viruses...Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression...Addgene depositors are making this possible in a range of organisms. Find CRISPR tools for use in genome...

  17. Plant Plasmids and Resources

    Type
    Collection
    ...window) for Targeted Gene Mutation in Rice - Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes...genome editing in monocot and dicot plants - Yao-Guang Liu. Plasmids for prime editing in plants (including...window) - The initiative promotes interdisciplinary exchange, open technologies, and responsible innovation...

  18. Neurodegeneration Research Collection

    Type
    Collection
    ...associated with the disease and implicate a wide range of cellular processes. The vast majority of cases... of nerve cells in the brain. Symptoms include changes in behavior and emotion, uncontrolled movements...include memory loss, deficits in decision-making and language abilities. The disease can be classified as early-onset...

  19. Plan Your Experiment

    Type
    Collection
    ... Edit Generate a specific user-defined sequence change in a particular gene, such as generating a point...was successful. PCR amplification, subcloning and Sanger sequencing (for HDR or NHEJ): Provides semi-quantitative...Validating your Genome Edit . Publications Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9...

  20. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Haeseleer F, Halterman C, Helback O, Hockemeyer D, Huang C, Huff S, Hunker A, Johansen N, Juneau Z, Kalmbach... R, Larsen R, Lee C, Lee AY, Leibly M, Lenz GH, Liang E, Lusk N, Malone J, Mollenkopf T, Morin E, Newman...Szelenyi ER, Trader C, Balaram P, van Velthoven CTJ, Chiang M, Mich JK, Dee N, Goldy J, Cetin AH, Smith K, ...
Showing: 21 - 40 of 83 results