...systems such as Arabidopsis, wheat, maize, rice, tomato, and a variety of other species. In addition to... genomes using genome engineering; validated in tomato, tobacco, M. truncatula , wheat, and barley - Daniel...metabolic engineering in plants (including tobacco and potato) - Scott Lenaghan. Joint Modular Cloning (JMC) ...

... serotype displays enhanced transduction of hematopoietic stem cells and microglia. The AAV6(dbY-F+T-V...High-Efficiency Transduction of Primary Human Hematopoietic Stem/Progenitor Cells by AAV6 Vectors: Strategies...

...enChIP-MS - A putative role of HDX as a negative regulator. Hamidian A, Vaapil M, von Stedingk K, Fujita ...ChIP combined with NGS analysis reveals genomic regulatory regions that physically interact with the Pax5.../srep03171. PubMed . Direct identification of insulator components by insertional chromatin immunoprecipitation...

... 58112 tdTomato-MAPTau-C-10 MAPT tdTomato CMV Parkinson's, FTD Michael Davidson 58113 tdTomato-MAPTau-...TARDBP GFP CMV ALS Zuoshang Xu 28205 wtTDP43tdTOMATOHA TARDBP HA, tdTomato CAG ALS Zuoshang Xu 28206 TDP43 ...ataxia Richard Possemato 102971 pLKO.1P shFXN_2 FXN Friedreich ataxia Richard Possemato 102975 pLKO.1P...pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 DCTN1 CMV ALS Trina ...tdTomato-MAPTau-N-10 MAPT tdTomato CMV Parkinson's, FTD Michael Davidson 58259 pBabe-Neuroserpin SERPINI1 Dementia Joan....1P shSOD1 SOD1 ALS Richard Possemato 103913 HTRA2_PDZ_1 HTRA2 GST tac Parkinson's Sachdev Sidhu 104448...James Trimmer 182922 hSOD1-FLAG SOD1 CMV ALS Philip Eaton 183984 huntingtin:TOC031-A07:C246751 HTT Flag CMV...

...without permanently modifying the genome dCas9-activator (such as dCas9-VP64) gRNA(s) targeting promoter...promoter elements of target gene Many different activators exist, including the multi-plasmid SAM system. Select...activate or repress a target gene using dCas9-activators or dCas9-repressors, gRNAs should be targeted...

...Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium have deposited...for cell migration research at Addgene. CMC investigators who have deposited plasmids include: Joan Brugge...

... plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects genome...collaborative program that brings together teams of investigators who will use state-of-the-art experimental and...

...and Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm...mutant alleles described in the initial Target Accelerator publications, focused on somatic variants found...

...Drugs (DREADDs) and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs are the most widely used...optogenetic element, making a bimodal opto-chemogenetic actuator. When the luciferase enzyme oxidizes its substrate...

...Find CRISPR plasmids to study the regulatory networks involved in retinal development....mouse retina. To aide in their studies of the regulatory networks involved in retinal development the ...

... mCherry 1 Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1, 2, 5, 8, 9, rg*, PHPeB Larsen 107787 AAV.TBG.PI.Cre.rBGe...

...important in cellular recognition processes. Laboratories interested in these approaches have created ... and secreted proteins Mouse sperm Mouse egg Laboratories using AVEXIS: Christian Sollner, Max Planck ...

...signed by an authorized signatory at the recipient institution. An authorized signatory is someone who can ...

...zebrafish Whole organism lineage tracing by combinatorial and cumulative genome editing - Jay Shendure...America. The Zebrafish Book - A guide for the laboratory use of zebrafish. CRISPRz - A database of CRISPR...

...elegans Dupuy Lab and Lehner Lab vectors - Tools for nematode transgenesis Insect / Baculovirus Polyhedrin pFastBac...vivo Dupuy Lab and Lehner Lab vectors - Tools for nematode transgenesis Rosenberg Lab Plasmids...

...Sun et al. Nat Methods. 2020 Oct 21. Glutamate indicators with improved activation kinetics and localization...with support from the BRAIN Initiative. Jackson Laboratory (Link opens in a new window) A collection of ...

...TCTTTTTGTGCTGCGCCAcccagcccataatcgaGTCTTCTACAGGCATGAA retinitis pigmentosa GTPase regulator b TAL3528 & TAL3529 TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA...TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA retinitis pigmentosa GTPase regulator b ORF15 TAL3530 & TAL3531 TAAGAGGGCGGAGCTTTTatctcgcaaagctctgCCCACTGAGTTACTTAGA...

...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective expression... RA, Goldowitz D, Wasserman WW, Simpson EM. A regulatory toolbox of MiniPromoters to drive selective expression...

...Plasmids Fluorescent Protein Purpose Principal Investigator Plasmids GFP GFP-dependent transcription factors...

...proteins for distribution to academic and nonprofit laboratories for research and educational use. Links to additional...

...family of at least 17 DNA binding transcriptional regulators with diverse roles in processes such as cell ...

...signals, cells, or groups of cells with either actuators or sensors. Chemogenetics Plasmids | Guide Use...

...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic...

...Transcriptional Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9...

...designed for in vivo transcrition controlled by the regulatory sequences of an RNA polymerase III transcribed...

...colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created...

...double-stranded DNA viruses known to cause mild to severe respiratory disease in humans. Advantages of adenoviruses...

... variant that has WRE, a post-transcriptional regulatory element that increases export to the cytoplasm...

... columns. Formulation Buffer After affinity chromatography, antibodies undergo a buffer exchange using...

...individual plasmids is a bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor...

...probably never happen. Yet with transcription activator-like (TAL) effectors, scientists in several biological...

...constructed using the cpFRET kit from the Pertz laboratory. Empty Vectors Encoding Fluorescent Proteins ...

...This library is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...

... genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas...

... and EGFP co-expression. See plasmid 21374 for dtomato version. Welm 21916 Tet-pLKO-neo 3rd inducible ...

...Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed a simple and scalable...

...Activate or Repress : dCas9 fused to transcriptional activators or repressors can modulate transcription in a...

...Two-Hybrid System Various Plasmids placO with lambda-C1 operator Beta-galactosidase Ann Hochschild Bacterial two-hybrid...

...Trypanosoma cruzi yes, cut S. pyogenes Neo Docampo tdTomato/pTREX-b 68709 Other/Trypanosoma cruzi none S. ...

...Link opens in a new window) together with the laboratory of NeuroMab Director, Dr. James Trimmer, have...